Severity of acute gastroenteritis in infants infected by G1 or G9 rotaviruses

Aupiais, Camille; Rougemont, Alexis de; Ménager, C.; Vallet, C.; Brasme, Jean-François; Kaplon, Jérôme; Pothier, Pierre; Gendrel, D.

doi:10.1016/j.jcv.2009.07.021

Cited by 17 publications

(8 citation statements)

References 29 publications

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“…These different detection rates may be explained by different conditions of the studies, such as the season of sampling and the sampling methods. For example, in other studies [24], samples were collected only from hospitalized children, whereas in our study, they were collected from inpatients and outpatients, which may have affected the prevalence rates.…”

Section: Discussionmentioning

confidence: 97%

The molecular epidemiology of circulating rotaviruses: three-year surveillance in the region of Monastir, Tunisia

et al. 2011

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BackgroundRotavirus infection is the most common cause of severe, dehydrating, gastroenteritis among children worldwide. In developing countries, approximately 1440 children die from rotavirus infections each day, with an estimated 527,000 annually. In infants, rotavirus is estimated to cause more than 2 million hospitalizations every year depending on the income level of the country.The purpose of this study was to estimate the proportion of rotavirus gastroenteritis and identify the distribution of circulating G and P genotype rotavirus strains among children consulting several dispensaries in the region of Monastir (outpatients departments) or admitted to Monastir University Hospital (inpatients department) with acute gastroenteritis.MethodsThis study was undertaken during a 3-year period from April 2007 to April 2010 in Tunisian children under 13 suffering from acute gastroenteritis. Group A rotaviruses were detected in stools by ELISA and genotyped using multiplex reverse transcription PCRs with type-specific primers on the basis of their outer capsid proteins. Statistical analyses were performed with SPSS software, version 19.ResultsOf the 435 stool samples from children with acute gastroenteritis, 27.6% were positive for rotavirus A. The predominant G type was G1 (37.5%), followed by G3 (25%), G2 (17.5%), G4 (12.5%), G9 (2.5%) and three mixed-G infections G3G4 (2.5%) were identified.Only P[8] (80.8%), P[4] (16.7%) and P[9] (0.8%) genotypes were found. The predominant single G/P combination was G1P[8] (37.5%), followed by G3P[8] (25%), G2P[4] (16.7%), G4P[8] (12.5%), G9P[8] (1.7%) and one case of the unusual combination G9P[9] (0.8%). The G-mixed types G3G4 combined with P[8] (2.5%). Infants less than 3 months of age were most frequently affected. The prevalence of rotavirus infection peaked in the winter season, when temperatures were low, and decreased in summer.ConclusionsRotavirus gastroenteritis is a common disease associated with significant morbidity, mortality, and economic burden. Epidemiological knowledge of rotavirus is critical for the development of effective preventive measures, including vaccines.These data will help to make informed decisions as to whether rotavirus vaccine should be considered for inclusion in Tunisia's National Immunisation Programme.

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Section: Discussionmentioning

confidence: 97%

The molecular epidemiology of circulating rotaviruses: three-year surveillance in the region of Monastir, Tunisia

et al. 2011

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“…7 In contrast, a study among French children hospitalized for rotavirus found no difference in severity, length of hospitalization, or the necessity for intravenous rehydration between G1 and G9 strains. 9 A third study of hospitalized rotavirus patients in India found that G1 caused more severe disease and more severe dehydration than G9 strains. 8 Several theories have been put forward to explain these inconsistencies.…”

Section: Discussionmentioning

confidence: 99%

Rotavirus genotype distribution during the pre-vaccine period in Bolivia: 2007–2008

Rivera

Forney

Castro

et al. 2013

International Journal of Infectious Diseases

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Summary Objectives Rotavirus is the most important etiology of severe diarrhea in Bolivia. The monovalent attenuated human oral rotavirus vaccine Rotarix® was introduced in Bolivia in 2008. We describe the molecular epidemiology of circulating rotavirus strains before vaccine introduction. Methods Two thousand one hundred thirty-five diarrheal samples were collected from hospitals in four Bolivian cities during 2007–2008. Forty-three percent (445 of 1030 rotavirus-positive samples) were analyzed for G and P genotypes. Among those, 331 were electropherotyped by polyacrylamide gel electrophoresis. Disease severity was quantified using a modified Vesikari scale. Results Among the 445 samples, five genotypes were found to be prevalent: G9P[8] (33%), G1P[6] (17%), G2P[4] (13%), G9P[6] (12%), and G1P[8] (4%). Co-infections with two or more strains accounted for 14% of samples. The most prevalent strain, G9, showed greater electropherotype diversity compared to other serogroups. Strain G1P[6] generally infected younger children and peaked later in the year than other strains. No particular genotype was associated with a higher severity score, though there was a significant difference in the duration of diarrhea between genotypes. Conclusions During the 2-year pre-vaccine period, substantial diversity of rotavirus co-circulating strains was observed. These data constitute a baseline against which changes in circulating strains post-vaccine introduction can be monitored.

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“…23 However, G9 association with increased severity is debatable as others reported that G1 had greater association with severe dehydration when compared with G9, 24,25 whereas other studies showed no difference of severity with any specific G-genotypes. [26][27][28] Differences in clinical severity associated G-genotypes may rely on the geographical origin of the strain or associated P-genotypes. 23 In addition, a significant difference of genotype distribution of rotavirus between outpatient and hospitalized cases may suggest that surveillance of rotavirus in only hospitalized severe diarrhea cases may not accurately represent distribution of rotavirus strains circulating in the area.…”

Section: Discussionmentioning

confidence: 99%

Genotypic Distribution of Rotavirus in Phnom Penh, Cambodia: An Association of G9 with More Severe Diseases

et al. 2017

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Abstract. Rotavirus causes significant morbidity and mortality among children worldwide. Stool samples from a previous hospital-based surveillance study to detect diarrhea etiology at the National Pediatric Hospital in Phnom Penh, Cambodia, by Meng and others in 2011 were tested for rotavirus by real-time reverse transcription polymerase chain reaction (PCR) targeting vp6 gene and characterized for G-and P-genotypes of positive samples based on vp7 and vp4 genes, respectively. Rotavirus was detected in 159/531 (30%) of children with diarrhea and none was detected in 287 nondiarrhea controls. All but three of the rotavirus-positive cases were children under the age of 2. The most common genotypes characterized by PCR and sequencing were G1P[8] (69%), G9P[8] (11%), and G2P[4] (11%). Genotype G9 was detected at a relatively high percentage that is consistent with the global trend and found to be associated with hospitalization. Data on disease burden and genotypic distribution are required information for the planning of rotavirus vaccine implementation in Cambodia.

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Severity of acute gastroenteritis in infants infected by G1 or G9 rotaviruses

Cited by 17 publications

References 29 publications

The molecular epidemiology of circulating rotaviruses: three-year surveillance in the region of Monastir, Tunisia

The molecular epidemiology of circulating rotaviruses: three-year surveillance in the region of Monastir, Tunisia

Rotavirus genotype distribution during the pre-vaccine period in Bolivia: 2007–2008

Genotypic Distribution of Rotavirus in Phnom Penh, Cambodia: An Association of G9 with More Severe Diseases

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