Severe weight loss in lambs infected with Giardia duodenalis assemblage B

Aloisio, F.; Filippini, G.; Antenucci, Pietro; Lepri, Elvio; Pezzotti, Giovanni; Cacciò, Simone M.; Pozio, Edoardo

doi:10.1016/j.vetpar.2006.06.023

Cited by 95 publications

(78 citation statements)

References 24 publications

(32 reference statements)

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“…Giardia is widely distributed in sheep with prevalence's of 1.5% to 55.6% reported (Feng and Xiao, 2011). The host specific assemblage E (livestock genotype) and the zoonotic assemblage A have been predominately reported in sheep (Giangaspero et al, 2005;Ryan et al, 2005;Santín et al, 2007;Geurden et al, 2008, Gómez-Muñoz et al, 2009Sprong et al, 2009;Yang et al, 2009;Lebbad et al, 2010;Nolan et al, 2010;Robertson et al, 2010;Sweeny et al, 2011;Feng and Xiao, 2011;Gómez-Muñoz et al, 2012;Zhang et al, 2012), although assemblage B has also been reported (Aloisio et al, 2006;Zhang et al, 2012). Previous studies conducted in Australia have examined lambs in Western Australia (WA) and Victoria (Vic) only (Ryan et al, 2005;Yang et al, 2009;Nolan et al, 2010;Sweeny et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Development of a quantitative PCR (qPCR) for Giardia and analysis of the prevalence, cyst shedding and genotypes of Giardia present in sheep across four states in Australia

Yang¹,

Jacobson²,

Gardner³

et al. 2014

Experimental Parasitology

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Section: Introductionmentioning

confidence: 99%

Development of a quantitative PCR (qPCR) for Giardia and analysis of the prevalence, cyst shedding and genotypes of Giardia present in sheep across four states in Australia

Yang¹,

Jacobson²,

Gardner³

et al. 2014

Experimental Parasitology

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“…Three genotypes of G. duodenalis have been identified in sheep, genotype E, and the two zoonotic genotypes, genotype A and B (Giangaspero et al, 2005, Ryan et al, 2005, Aloisio et al, 2006, van der Giessen et al, 2006Castro-Hermida et al, 2007;Santin et al, 2007;Geurden et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Prevalence and molecular characterisation of Cryptosporidium and Giardia species in pre-weaned sheep in Australia

Yang

Jacobson

Gordon

et al. 2009

Veterinary Parasitology

100

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A c c e p t e d M a n u s c r i p t 2 AbstractA total of 477 fecal samples from pre-weaned sheep from 5 different farms in the south west of Western Australia were screened for the presence of Cryptosporidium and Giardia using PCR. There were substantial differences in prevalence between the farms and overall prevalence was 24.5% and 11.1% respectively for Cryptosporidium and Giardia. At the 18S locus, 66 Cryptosporidium positives were identified, the majority of which were C. bovis (n = 52), followed by the cervid genotype (n= 10) and C. parvum (n = 2). At a second diagnostic locus, using C. parvum and C. hominis specific qPCR primers, 63 C. parvum positives were identified, some of which were co-infections with C. bovis. The C. parvum/C. hominis qPCR was more sensitive than the nested 18S PCR at detecting C. parvum. This may be due to the low numbers of oocysts present, as quantitation data indicated that all the C. parvum detected was present in low numbers (1-10 oocysts). It may also be that using C. parvum-specific primers is necessary to determine the true prevalence of C. parvum. Amongst Giardia positive isolates, G. duodenalis genotype E (livestock) was the most prevalent (36/53), with G.duodenalis genotype A detected in 5 positive isolates. There were also 11 mixed A and E infections detected. The findings of the present study indicate that pre-weaned lambs are not an important source of zoonotic Giardia genotypes in Australia but may be an important source of zoonotic Cryptosporidium.

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“…Giardiosis in cattle is associated with a high morbidity, significant production loss and potential transmission to other hosts [1,4,18]. Prevalence rates may vary according to the age of the animals, design of the study, diagnostic techniques, geographical location, climatological conditions and management practices [4,18].…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Molecular Characterization of Giardia duodenalis in Calves in Turkey

Gültekin

Ural

Aysul

et al. 2017

Acta Scientiae. Vet.

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Background: Giardia duodenalis (G. duodenalis) is an ubiquitous, flagellated intestinal protozoan with major public health significance worldwide. Limited data are available on the epidemiology of G. duodenalis in dairy cattle from Turkey. Determining the zoonotic potential of the Giardia infection requires molecular characterization. The aim of the present study was to investigate the prevalence and to molecularly characterize G. duodenalis in calves less than three months of age in Aydin, Aegean region of Turkey. Materials, Methods & Results: The study was conducted on different dairy farms in the south-western part of the Turkey, Aegean Region, Aydin. A total of 198 Holstein Friesian calves less than three months of age, of both sexes were enrolled into the study. Faecal samples from each calf were collected manually from the rectum using a disposable latex glove. The consistency of collected samples was recorded as diarrhoeic or non-diarrhoeic. Diagnosis of G. duodenalis infection was made microscopically by detection of cysts in the faecal samples. One hundred and sixteen (58.5%) of the 198 faecal samples were diarrheic. Giardia cysts were found in 27 (23.28%) of the diarrheic samples and in 8 (9.76%) of nondiarrheic samples (P < 0.05). The overall prevalence of giardiosis in calves was determined as 17.67%. The prevalence of Giardia genotypes was identified by DNA sequence analysis of the beta-giardin gene for every PCR positive sample. The beta-giardin nested PCR assay was revealed assemblage A and sub-genotype A3 was detected in all of 35 samples (100%). Discussion: The highest prevalence of Giardia infection in calves is reported at the age between 1 and 6 months, and the prevalence shows decreased rate from the age of 6 months. The present study was conducted in Aydin, a province of south-western Turkey in the Aegean Region, and the overall prevalence from a total of 198 dairy calves was 17.67%. The prevalence rate in calves with diarrhoea was higher and reached up to 23.28%, whereas it was 9.76% in non-diarrhoeic calves. A prevalence study with molecular characterization of G. duodenalis isolates in cattle has not yet been reported from Turkey. Molecular studies have shown that mostly assemblage E predominates in cattle, but recent studies denoted that assemblage A is increasingly being detected and might be more widespread than expected before. In the present study, Giardia positive samples identified with a beta-giardin nested PCR assay. The sub-genotype A3 was identified in all samples. The same sub-genotype was identified in human and dog samples from different countries. Furthermore, sub-genotype A3 was found in humans and dogs from Turkey. In this context, results of the present study suggested an important role of calves as potential reservoirs of human infections in Turkey. In conclusion, epidemiological data revealed that G. duodenalis infection is frequent in calves with diarrhoea in Aydin, Turkey. The presence of the potentially zoonotic sub-genotype A3 and high prevalence of Giardi...

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Severe weight loss in lambs infected with Giardia duodenalis assemblage B

Cited by 95 publications

References 24 publications

Development of a quantitative PCR (qPCR) for Giardia and analysis of the prevalence, cyst shedding and genotypes of Giardia present in sheep across four states in Australia

Development of a quantitative PCR (qPCR) for Giardia and analysis of the prevalence, cyst shedding and genotypes of Giardia present in sheep across four states in Australia

Prevalence and molecular characterisation of Cryptosporidium and Giardia species in pre-weaned sheep in Australia

Prevalence and Molecular Characterization of Giardia duodenalis in Calves in Turkey

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