Severe teratozoospermia and male age increase levels of sperm apoptosis in infertile patients

Uriondo, H.; Sedó, C. Alvarez; Gil, M.V.; Frazer, P.; Serna, J.; Nodar, F.

doi:10.1016/j.fertnstert.2011.07.275

Cited by 3 publications

(4 citation statements)

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“…This observation is consistent with previous reports elsewhere (14,15) even though different markers of apoptosis were evaluated by these authors. Whereas Colin and coworkers (15) measured plasma membrane translocation of phosphatidyserine (marker of early apoptosis), while Uriondo and colleagues (16) evaluated caspase 3, DNA fragmentation and phosphatidyserine in seminal plasma of men of different age groups.…”

Section: Discussionsupporting

confidence: 93%

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Effect of senescence on some apoptosis and oxidative stress markers in infertile normozospermic and oligospermic men: A cross-sectional study

Emokpae

Chima

2018

IJRM

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Background: Male senescence may affect testicular function, sperm indices and generation of high levels of oxidants and apoptosis. Objective: This study evaluates the effect of male age on the expression of some apoptosis and oxidative stress markers in seminal fluid of males investigated for infertility in a tertiary health institution in Nigeria. Materials and Methods:In this cross-sectional study, 122 men aged 20-60 yr who were investigated for infertility and were stratified according to age into four groups. Seminal plasma caspase 3, cytochrome C, and total antioxidant capacity (TAC) were assayed by ELISA technique, while manual semen analysis was performed according to WHO standard. Results: Seminal caspase 3, and cytochrome C activity increased while TAC and sperm indices decreased with increasing age. Cytochrome C (r=0.288; p=0.002) and caspase 3 (r=0.250; p=0.05) correlated significantly with age in normospermia while cytochrome C (r=0.314; p=0.02), caspase 3 (r=0.268; p=0.05), TAC (r=-0.342; p=0.01) and morphology percentage (r=-0.414; p=0.002) correlated with age in oligospermic infertile males. Conclusion: The measured apoptotic markers increased with increasing age while TAC and sperm indices decreased with increasing age of subjects evaluated. Although the levels of measured apoptosis and oxidative stress markers correlated with age in normozospermia, the effect on sperm indices was severe among oligospermia compare to normozospermia. Therefore, these markers may be assayed in aged men attending fertility clinics.

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Section: Discussionsupporting

confidence: 93%

“…They observed that advancing age was significantly and positively correlated with phosphatidyserine and Annexin-v binding. Although correlation for Annexin-V binding was not statistically significant, a clear trend of increased DNA fragmentation was observed in the older study participants with 40 yr as the age threshold (15).…”

Section: Discussionmentioning

confidence: 60%

Effect of senescence on some apoptosis and oxidative stress markers in infertile normozospermic and oligospermic men: A cross-sectional study

Emokpae

Chima

2018

IJRM

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“…Semen quality is usually expressed in terms of sperm concentration, motility, and morphology ( WHO, 2010 ). Our group previously reported that these parameters, specifically morphology and motility, were closely related to DNA alterations ( Uriondo et al. , 2011 , Alvarez Sedó et al , 2012 ).…”

Section: Discussionmentioning

confidence: 90%

Effect of sperm DNA fragmentation on embryo development: clinical and biological aspects

Sedó¹,

Bilinski²,

Lorenzi³

et al. 2017

JBRA Assisted Reproduction

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ObjectiveThe aim of this study was to investigate the effect of sperm DNA fragmentation on fertilization rate, embryo development (blastulation rate), and pregnancy outcomes for ICSI cycles performed in a cohort of couples using donor eggs and to assess the remaining embryos that were not transferred or frozen for apoptotic markers.MethodsEighty-two women (egg recipients) were included in the study (2016) were included in the study. The recipients' mean age was 41.8±5.1 y/o (36-49), while the egg donors' mean age was 30.8±2.1 y/o (27-33). Even though donor egg cycles with frozen sperm samples are performed regularly in our center, 35 cycles were done using fresh sperm samples. The mean age of the males involved in the procedure was 40.1±5.2 y/o. Fertilization, blastulation, and pregnancy rates were assessed. The patients were divided into two groups, TUNEL <15% and ≥15%. In arrested embryos, ICC was performed to detect cleaved caspase-3, survivin, TUNEL, and DNA. The Student's t-test was used in between-group comparisons. The Mann-Whitney U-test was used to assess homogeneity. Pearson's correlation coefficient was also calculated. p<0.05 was considered statistically significant.Results This study showed that there is a negative correlation (R=-0.5) between DNA fragmentation and blastulation rate. High levels of DNA fragmentation were associated with low blastulation and pregnancy rates (per transfer); however, fertilization rate was not affected. Samples with higher levels of DNA fragmentation were associated with higher levels of DNA fragmentation in blastomeres without activating the apoptotic pathway (9.1% vs. 15.9%) (p<0.05). Blastomeres from samples with high DNA fragmentation activated the apoptotic pathway in higher levels than samples with TUNEL <15% (16.4% vs. 21.9%) (p<0.05).ConclusionSperm DNA fragmentation was negatively correlated with blastulation and pregnancy rates even in good quality oocytes. High levels of DNA damage promote embryo arrest and induce the activation of the apoptotic pathway.

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“…These data concur with the report earlier [53]; this study found an increase in testicular apoptosis between 12 and 24 months old in rats. The correlation of the expression of annexin V with apoptotic markers in the sperm of men aged over 40 years old has been previously reported [54]. Furthermore, the increase in those markers was found to be directly proportional to the age of the patients [55][56][57].…”

Section: Discussionmentioning

confidence: 58%

Efecto de la restricción calórica sobre la conducta sexual masculina, la calidad espermática y las hormonas metabólicas en la rata Wistar longeva

López de Jesús

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Arenas Ríos, cede los derechos del trabajo de tesis a la Universidad Autónoma Metropolitana para su difusión, con fines académicos y de investigación.Los usuarios de la información no deben reproducir el contenido textual, gráficas o datos del trabajo sin permiso expreso del autor o del Comité Tutoral del trabajo. Con el fin de solicitar autorización, los usuarios podrán escribir al correo electrónico

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Severe teratozoospermia and male age increase levels of sperm apoptosis in infertile patients

Cited by 3 publications

References 0 publications

Effect of senescence on some apoptosis and oxidative stress markers in infertile normozospermic and oligospermic men: A cross-sectional study

Effect of senescence on some apoptosis and oxidative stress markers in infertile normozospermic and oligospermic men: A cross-sectional study

Effect of sperm DNA fragmentation on embryo development: clinical and biological aspects

Efecto de la restricción calórica sobre la conducta sexual masculina, la calidad espermática y las hormonas metabólicas en la rata Wistar longeva

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