Severe molluscum contagiosum infection in a patient with human T cell lymphotrophic (HTLV-III) disease

Redfield, Robert; James, William D.; Wright, David C.; Brown, Charles D.; Salahuddin, S. Z.; Markham, Phillip D.; Sarngadharan, M. G.; Folks, T; Gallo, Robert C.

doi:10.1016/s0190-9622(85)80397-2

Cited by 60 publications

(11 citation statements)

References 19 publications

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“…Severe and persistent MCV infections are reported to be common in patients who are immunosuppressed [Solomon and Telner, 1966;Ganpule and Garretts, 1971;Pauly et al, 19781. Since 1985 there have been numerous reports concerning the severity of molluscum contagiosum infection in AIDS patients [Lombardo, 1985;Sarma and Weilbaecher, 1985;Redfield et al, 1985;Katzman et al, 19871. The incidence and the reason for the severity of the infection in these patients is not known, but it has been suggested that it may be due to the tendency of MCV to persist in a latent phase or to aspects of cell-mediated immunity affected by HIV (i.e., depletion of the Thelper cell subpopulation).…”

Section: Discussionmentioning

confidence: 99%

Molecular epidemiology of Australian isolates of molluscum contagiosum

Thompson

Zwart-Steffe

Biggs

1990

Journal of Medical Virology

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Molluscum contagiosum lesions obtained from 75 Australian patients, 22 (29%) of whom were HIV positive, were examined by restriction endonuclease analysis and Southern blot hybridisation using radiolabelled and digoxigenin-labelled MCV DNA probes. The isolates were classified (MCV 1, 1v, 2, and 2v) on the basis of these results, which were in turn correlated with the clinical features of each lesion. A total of 44 (59%) of the lesions contained MCV 1 or 1v, 24 (32%) contained MCV 2 or 2v, three (4%) contained multiple MCV types, whereas four (5%) of lesions submitted contained no detectable MCV DNA. The ratio of MCV 1/1v to MCV 2/2v was determined to be approximately 1.75:1. There were substantial differences between the distribution of MCV types 1 and 2 among patients of different age groups, but no significant relationship between MCV type and the sex of the patient was found. MCV 2 was more frequently detected in lesions from anogenital areas and in immunosuppressed (HIV-positive) patients, and MCV 1 was more commonly isolated from skin rather than genital lesions, but neither association was statistically significant. Fragment F (10 kbp) obtained from the genome of MCV 1 was capable of differentiating MCV 1 from MCV 2 when used as a probe in hybridisation experiments with BamHI cut samples and may be useful when small amounts of lesion prevents differentiation by direct visualisation of restriction endonuclease fragments.

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Section: Discussionmentioning

confidence: 99%

Molecular epidemiology of Australian isolates of molluscum contagiosum

Thompson

Zwart-Steffe

Biggs

1990

Journal of Medical Virology

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“…In individuals with normal immune function disease is usually self-limiting and is generally regarded as trivial. However, immunocompromised persons may be afflicted by severe MCV infections which are often refractory to the usual forms of therapy [Lombardo, 1985;Redfield et al, 1985;Katzman et al, 1987]. Since the infection cannot be transmitted to laboratory animals and a reliable in vitro system for MCV replication has yet to be established [Postlethwaite, 1970;McFadden et al, 1979], the epidemiology and pathogenesis of this disease remain largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Identification and typing of molluscum contagiosum virus in clinical specimens by polymerase chain reaction

Thompson

1997

J. Med. Virol.

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A polymerase chain reaction (PCR) which enables the detection of molluscum contagiosum virus (MCV) genomes in either fresh or formalin-fixed clinical specimens is described. The primers used were designed to amplify a 167 bp region of the 3.8 kbp HindIII fragment K of the MCV 1 genome. The ability of this PCR to detect three common MCV types (1, 1v and 2) in clinical specimens was confirmed using frozen extracts from 75 molluscum lesions, and digests of single sections of 11 formalin-fixed, paraffin-embedded lesions; all of which had been previously typed by Southern hybridisation. In addition, 2 specimens previously negative by hybridisation were shown to be positive for MCV DNA by PCR. Confirmation of the identity of the PCR products and distinction between the two major MCV types (MCV 1/1v versus MCV 2) was achieved by comparison of the results of cleavage with the restriction endonucleases Hhal and Sacl. Sequencing of the PCR products revealed complete homology between MCV 1 and 1v, but minor nucleotide variations between MCV 1/1v and MCV 2 were identified. As well as providing a highly sensitive means of diagnosis, the technique may also prove useful for investigations into the pathogenesis, epidemiology and natural history of molluscum contagiosum infection.

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“…A poxvirus causes molluscum contagiosum, a sexually transmitted papular skin lesion, which in immunocompetent individuals is usually self-limiting and resolves spontaneously in 3-12 months. In AIDS patients, the disease is more extensive and often occurs on the face (Redfeld et al, 1985). In the absence of exogenous immunosuppression, extensive molluscum lesions in unusual locations with unusually rapid recurrence despite diligent therapy suggest AIDS (Katzman et al, 1985;Lombardo, 1985;Sarma and Weilbaecher, 1985).…”

Section: Poxuirusmentioning

confidence: 99%

Viral infections in the acquired immunodeficiency syndrome

Miller

Howell

1988

J. Elec. Microsc. Tech.

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The following communication is a tripartite synopsis of the role of viral infection in the acquired immunodeficiency syndrome (AIDS). The first section describes the impact of viral opportunistic infection in AIDS; for each virus, clinical presentation and diagnosis, laboratory diagnostic approaches (with emphasis on electron microscopy), and therapeutic interventions attempted to date are discussed. The second segment explores current theories on the pathogenesis of AIDS, and describes diagnostic and therapeutic approaches to the syndrome itself. The final section catalogues ultrastructural anomalies in the cells of AIDS patients, many of which have been mistakenly identified as etiologic agents.

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Severe molluscum contagiosum infection in a patient with human T cell lymphotrophic (HTLV-III) disease

Cited by 60 publications

References 19 publications

Molecular epidemiology of Australian isolates of molluscum contagiosum

Molecular epidemiology of Australian isolates of molluscum contagiosum

Identification and typing of molluscum contagiosum virus in clinical specimens by polymerase chain reaction

Viral infections in the acquired immunodeficiency syndrome

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