Severe disruption of the cytoskeleton and immunologically relevant surface molecules in a human macrophageal cell line in microgravity—Results of an in vitro experiment on board of the Shenzhou-8 space mission

Paulsen, Katrin; Tauber, Svantje; Goelz, Nadine; Simmet, Dana M.; Engeli, Stephanie; Birlem, Maria; Dumrese, Claudia; Karer, Anissja; Hunziker, Sandra; Biskup, Josefine; Konopasek, Shalimar; Suh, Durie; Hürlimann, Eva; Signer, C; Wang, Anna; Sang, Chen; Grote, Karl‐Heinrich; Zhuang, Fengyuan; Ullrich, Oliver

doi:10.1016/j.actaastro.2013.06.007

Cited by 30 publications

(32 citation statements)

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“…To corroborate the relevance of the results obtained with murine BV-2 microglial cells, we investigated a human macrophage-like cell system. Therefore, human monocytic U937 cells were differentiated into macrophage-like cells [ 23 ] and human M2 macrophages were differentiated from blood mononuclear cells. Before the experiment, differentiated macrophage-like cells were detached, resuspended in fresh medium, and filled into 1 mL standardized serological pipettes for the clinostat.…”

Section: Resultsmentioning

confidence: 99%

“…Inside the EUEs, the slides were bedded in 0.5 mL fully CO 2 saturated RPMI 1640 medium with 10% HS, 2 mM glutamine, 100 U/mL penicillin, 100 μ g/mL streptomycin, and 250 ng/mL amphotericin B (PromoCell). Bellow 1, 3, and 5 of the EUEs [ 23 ] were filled with RPMI 1640 medium, 10% HS, 100 U/mL penicillin, 100 μ g/mL streptomycin, 250 ng/mL amphotericin B, 2 mM glutamine, 1% PFA, and 0.6% sucrose. Bellow 2, 4, and 6 of the EUEs [ 23 ] were filled with PBS, 100 U/mL penicillin, 100 μ g/mL streptomycin, and 250 ng/mL amphotericin B.…”

Section: Methodsmentioning

confidence: 99%

“…Bellow 1, 3, and 5 of the EUEs [ 23 ] were filled with RPMI 1640 medium, 10% HS, 100 U/mL penicillin, 100 μ g/mL streptomycin, 250 ng/mL amphotericin B, 2 mM glutamine, 1% PFA, and 0.6% sucrose. Bellow 2, 4, and 6 of the EUEs [ 23 ] were filled with PBS, 100 U/mL penicillin, 100 μ g/mL streptomycin, and 250 ng/mL amphotericin B. Two EUEs (6 chambers) were prepared for the μ g-position, and one EUE (3 chambers) was prepared for the 1 g position.…”

Section: Methodsmentioning

confidence: 99%

“…The ground control experiment was executed analogously to the flight scenario. Details about the experiment were published previously [ 23 ].…”

Section: Methodsmentioning

confidence: 99%

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Regulation of ICAM-1 in Cells of the Monocyte/Macrophage System in Microgravity

Paulsen

Tauber

Dumrese

et al. 2015

BioMed Research International

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Cells of the immune system are highly sensitive to altered gravity, and the monocyte as well as the macrophage function is proven to be impaired under microgravity conditions. In our study, we investigated the surface expression of ICAM-1 protein and expression of ICAM-1 mRNA in cells of the monocyte/macrophage system in microgravity during clinostat, parabolic flight, sounding rocket, and orbital experiments. In murine BV-2 microglial cells, we detected a downregulation of ICAM-1 expression in clinorotation experiments and a rapid and reversible downregulation in the microgravity phase of parabolic flight experiments. In contrast, ICAM-1 expression increased in macrophage-like differentiated human U937 cells during the microgravity phase of parabolic flights and in long-term microgravity provided by a 2D clinostat or during the orbital SIMBOX/Shenzhou-8 mission. In nondifferentiated U937 cells, no effect of microgravity on ICAM-1 expression could be observed during parabolic flight experiments. We conclude that disturbed immune function in microgravity could be a consequence of ICAM-1 modulation in the monocyte/macrophage system, which in turn could have a strong impact on the interaction with T lymphocytes and cell migration. Thus, ICAM-1 can be considered as a rapid-reacting and sustained gravity-regulated molecule in mammalian cells.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The ground control experiment was executed analogously to the flight scenario. Details about the experiment were published previously [ 23 ].…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Regulation of ICAM-1 in Cells of the Monocyte/Macrophage System in Microgravity

Paulsen

Tauber

Dumrese

et al. 2015

BioMed Research International

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“…In unseren Studien untersuchten wir Signaltransduktionskaskaden [3,[6][7][8], Adhäsionsmoleküle [9][10][11], Stoffwechsel [11], funktionelle Parameter [12,13], Genexpressionsreaktionen [6,[14][15][16][17][18][19]…”

Section: Erkenntnisse Aus Unseren Studienunclassified

Schnelle zelluläre Reaktion und Anpassung an Schwerelosigkeit

Ullrich

Thiel

2019

Flug u Reisemed

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ZUSAMMENFASSUNGZelluläre Prozesse werden durch Änderungen der Gravitationskraft in vielfältiger Weise beeinflusst. In unseren Studien konnten wir in verschiedenen zellulären Systemen und in verschiedenen Forschungsplattformen (2D Klinostat, Parabelflüge, suborbitale Forschungsraketen, Internationale Raumstation) nachweisen, dass Reaktionen und Anpassungsprozesse bereits innerhalb von Sekunden bis Minuten nach Beginn der veränderten Schwerkraft auftreten. Diese schnellen Prozesse fanden sich in der Genexpression, der Zellzykluskontrolle, der Signaltransduktion, der Zytoskelettorganisation und des oxidativen Burst. Es stellt sich die Frage nach der Ursache der Transduktion einer unspezifischen Kraft in eine hochspezifische zelluläre Antwort, die in der Spezifität der zellulären Eigenschaften begründet liegen kann. Es ist denkbar, dass die Gravitationskraft der Erde die Chromatinarchitektur und deren Zugänglichkeit stabilisiert und eine homöostatische Bedingung für die Genexpression darstellt. Die Untersuchung mechanobiologischer Mechanismen der Genexpression in Schwerelosigkeit kann die Aufdeckung fundamentaler Prinzipien ermöglichen, wie mechanische Kräfte die Zellfunktion regulieren.

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Use of In Vitro Cell Culture Models to Understand the Cellular and Molecular Basis of Immune Dysfunction During Spaceflight

Tauber

Choukèr

et al. 2016

Effect of Spaceflight and Spaceflight Analogue Culture on Human and Microbial Cells

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Severe disruption of the cytoskeleton and immunologically relevant surface molecules in a human macrophageal cell line in microgravity—Results of an in vitro experiment on board of the Shenzhou-8 space mission

Cited by 30 publications

References 50 publications

Regulation of ICAM-1 in Cells of the Monocyte/Macrophage System in Microgravity

Regulation of ICAM-1 in Cells of the Monocyte/Macrophage System in Microgravity

Schnelle zelluläre Reaktion und Anpassung an Schwerelosigkeit

Use of In Vitro Cell Culture Models to Understand the Cellular and Molecular Basis of Immune Dysfunction During Spaceflight

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