Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) immunoglobulins using chemiluminescence immunoassay and its correlation with neutralizing antibodies

Gaber, Yasmine; Alem, Shereen Abdel; Musa, Sherief; Amer, Khaled; Elnagdy, Tarek; Hassan, Waël; Abdelrahman, Raafat Zaher; Gad, Ahmed F.; Ali, Mohamed A.; Badary, Hedy A; Shawky, Shereen; Talaat, Hala; Kassem, Abdel Meguid; Fouad, Rabab

doi:10.1016/j.virusres.2022.198852

Cited by 3 publications

(2 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We detected IgG antibodies specific to the receptor-binding domain (RBD) of the spike protein on SARS-CoV-2 to evaluate immune response post vaccination. AntiS1-SARS-CoV-2 IgG antibodies showed a correlation with neutralizing antibodies and can be used to estimate the presence of protective immunity in asymptomatic and previously symptomatic SARS-CoV-2 infection ( 24 , 25 ).…”

Section: Resultsmentioning

confidence: 99%

Post-vaccination SARS-CoV-2 seroprevalence in children aged 3-11 years and the positivity in unvaccinated children: A retrospective, single-center study

Dai

et al. 2022

Front. Immunol.

View full text Add to dashboard Cite

ObjectiveTo analyze the positivity and levels of SARS-CoV-2 antibodies in vaccinated children to evaluate the humoral immune response of vaccination on pediatric population. Analysis on the causes of antibody positivity in unvaccinated children.MethodsA retrospective study was conducted on children who were admitted to the Children’s Hospital Affiliated to Capital Institute of Pediatrics. The clinical data of serological testing of SARS-CoV-2 immunoglobulin M (IgM) and IgG antibodies were collected from SARS-CoV-2 vaccinated or unvaccinated children with no evidence of prior novel coronavirus infection. Chemiluminescence immunoassay was utilized for the in vitro determination of SARS-CoV-2 antibodies.ResultsA total of 3,321 healthy children aged 6-11 years received two doses of inactivated SARS-CoV-2 vaccine. At 1 month after the second dose, the positive rate (96.5%) and levels [8.039 (interquartile range (IQR), 6.067-9.098)] of SARS-CoV-2 IgG antibodies reached the peak and remained at a high level for 2-3 months, after which the positive rate and level of vaccine-induced IgG antibody gradually decreased. Compared with 1 month after the second dose of vaccine, the positive rate of IgG antibody decreased to 70.4% at 7 months, and the antibody level decreased by 69.0%. A total of 945 children aged 3-5 years received one or two doses of inactivated vaccine. The positive rate and levels of SARS-CoV-2 IgG antibody in participants remained high for 3 months after vaccination. There was no gender-based difference in positive rate of IgG antibody in children aged 3-11 years old (P>0.05). Among the 5,309 unvaccinated children aged 0 day to 11 years, 105 (2.0%) were positive for SARS-CoV-2 IgG antibody, which was associated with passive infusion. The maternal humoral response to COVID-19 vaccination in noninfected pregnant women was transferred through the placenta to the fetus, and some children obtained SARS-CoV-2-positive antibodies through blood transfusion.ConclusionsInactivated SARS-CoV-2 vaccines could induce robust humoral immune response that gradually declined within several months after the second dose. Therefore, it helps to determine whether children receive a booster dose and elicit a long-term memory immune response. Positive SARS-CoV-2 antibodies in unvaccinated children were associated with passive IgG antibody infusion.

show abstract

Section: Resultsmentioning

confidence: 99%

Post-vaccination SARS-CoV-2 seroprevalence in children aged 3-11 years and the positivity in unvaccinated children: A retrospective, single-center study

Dai

et al. 2022

Front. Immunol.

View full text Add to dashboard Cite

show abstract

“…Medical interventions did not end the SARS pandemic, but infection control measures did (2). Recently, certain SARS-CoV-like viruses can infect human cells in bats without further adaptation, which suggests that SARS could re-emerge (5,26).…”

Section: Important Characteristics Of Coronavirusmentioning

confidence: 99%

The Essential Points Regarding Laboratory Biosafety to Prevent the Spread of SARS-CoV-2

Mafi

Ghareshiran

Hamidi

et al. 2023

J Kermanshah Univ Med Sci

View full text Add to dashboard Cite

: A current biosafety and biosecurity crisis, SARS-CoV-2/COVID-19, threatens public health, making it crucial to assess and follow optimal laboratory conditions to prevent any contamination and spread of the lethal virus. Much medical staff have been lost to the COVID-19 pandemic. The dimensions of laboratory biosafety were examined in this study to reduce risks associated with COVID-19 treatment. In addition, this research primarily focuses on assessing laboratory biosafety regarding emerging coronaviruses. This research will determine which aspects need to be addressed to address the risks and implement them in the correct order and at the right time to be as educational as possible.

show abstract

Determining the SARS-CoV-2 Anti-Spike Cutoff Level Denoting Neutralizing Activity Using Two Commercial Kits

et al. 2022

View full text Add to dashboard Cite

Background: The viral neutralization assay is the gold standard to estimate the level of immunity against SARS-CoV-2. This study analyzes the correlation between the quantitative Anti-SARS-CoV-2 QuantiVac ELISA (IgG) and the NeutraLISA neutralization assay. Methods: 650 serum samples were tested for both SARS-CoV-2 anti-spike (anti-S) immunoglobulin G (IgG) and neutralizing antibodies (nAbs) using kits by EUROIMMUN, Germany. Results: There was a significant correlation between levels of anti-S and nAbs (Spearman’s rho = 0.913). Among the positive samples for anti-S, 77.0% (n = 345) were positive for nAbs. There was a substantial agreement between anti-S and nAbs (Cohen’s kappa coefficient = 0.658; agreement of 83.38%). Considering NeutraLISA as a gold standard, anti-S had a sensitivity of 98.57%, specificity of 65.66%, NPV of 97.5%, and PPV of 77.0%. When the anti-S titer was greater than 18.1 RU/mL (57.9 BAU/mL), nAbs were positive, with a sensitivity of 90.0% and specificity of 91%. Conclusions: A titer of SARS-CoV-2 anti-S IgG can be correlated with levels of nAbs.

show abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) immunoglobulins using chemiluminescence immunoassay and its correlation with neutralizing antibodies

Cited by 3 publications

References 38 publications

Post-vaccination SARS-CoV-2 seroprevalence in children aged 3-11 years and the positivity in unvaccinated children: A retrospective, single-center study

Post-vaccination SARS-CoV-2 seroprevalence in children aged 3-11 years and the positivity in unvaccinated children: A retrospective, single-center study

The Essential Points Regarding Laboratory Biosafety to Prevent the Spread of SARS-CoV-2

Determining the SARS-CoV-2 Anti-Spike Cutoff Level Denoting Neutralizing Activity Using Two Commercial Kits

Contact Info

Product

Resources

About