Several distinct properties of the IgE repertoire determine effector cell degranulation in response to allergen challenge

Isoallergen Variations Contribute to the Overall Complexity of Effector Cell Degranulation: Effect Mediated through Differentiated IgE Affinity

“…We have previously reported how individual factors, such as concentration, affinity, and clonality of the IgE repertoire, are crucial determinants of the outcome of IgE-allergen complexmediated effector cell degranulation (15). In this study, we demonstrate that individual IgE Abs derived from an HDM-allergic patient's IgE repertoire are able to bind recombinant constructs of naturally occurring Der p 2 isoforms with a broad range of affinities.…”

“…Basophil degranulation experiments were essentially carried out as previously described (15) and illustrated in Supplemental Fig. 6.…”

“…3B, marked by green and red circles) bound by two chimeric rIgE Abs, named H1 and H12:H7, having their Der p 2 binding domains cloned from mice (15). Two surface-exposed variable amino acids (Fig.…”

“…We have previously used rIgE Abs, including the clones described in this study, to show how individual properties of the IgE repertoire affect effector cell degranulation upon allergen challenge (15). In that publication, we stated that IgE clones C-K were cloned by the phage display method, but without a detailed description (only mentioned "as described in detail elsewhere").…”

Molecular basis of allergen cross-reactivity: Non-specific lipid transfer proteins from wheat flour and peach fruit as models

“…However, the information about conformational epitopes, which are of great importance in highly resistant proteins such as nsLTPs, would be lost due to the own limitation of the technique. The inherent restrictions of this approach suggest that potentially relevant IgE-binding regions of the allergen studied, particularly conformational epitopes formed by sequentially discontinuous amino acid residues, cannot be detected (Aalberse, 2000;Christensen et al, 2008). The production of hypoallergenic forms has been used to analyze structural epitopes.…”