Seventeen novel microsatellite markers from an enriched library of the pest species Bactrocera dorsalis sensu stricto

Abstract

Seventeen novel microsatellite markers were isolated and characterized from an enriched DNA library of the pest species Bactrocera dorsalis sensu stricto . The polymorphism of these loci was tested in individual fruit flies from a Thai mass-rearing strain and three wild Asian populations: two from Thailand and one from Myanmar. Allele numbers per locus ranged from two to 15. In the natural populations, a high level of polymorphism was detected in all loci suggesting the usefulness of these markers to quantify the genetic variation in this highly invasive insect.

Characterization and evaluation of microsatellite markers in a strain of the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae), with a genetic sexing character used in sterile insect population control

“…Amplifications of six B. dorsalis microsatellite loci; Bd1, Bd9, Bd15, Bd19, Bd42, and Bd85B (Aketarawong et al, 2006); and five B. papayae loci; Bp58, Bp73, Bp125, Bp173, and Bp181 (Shearman et al, 2006) were performed with each DNA sample by polymerase chain reaction (PCR) according to the PCR conditions as described in Aketarawong et al (2006). PCR products were resolved on 6% or 12% polyacrylamide electrophoresis gels, depending on microsatellite locus size.…”

“…Monitoring in ecological pest management, such as identification of individual flies, is often performed using such markers (Waits et al, 2001). In recent years, high resolution microsatellite markers for B. dorsalis were designed (Aketarawong et al, 2006). Many of these markers revealed high levels of polymorphism in different geographical populations, either at micro-or macro-geographical levels (Aketarawong et al, 2007).…”

GENETIC DIVERSITY OF NATURAL POPULATIONS OF Bactrocera occipitalis (Bezzi) AND B. philippinensis Drew and Hancock (Diptera: Tephritidae) IN SELECTED MANGO PRODUCING AREAS IN THE PHILIPPINES USING MICROSATELLITES

“…This means that on the average, the populations were 49 % deficient of heterozygotes. HWE deviations as a result of heterozygote deficiency may be attributed to null alleles, inbreeding, finite sample size, a chance of sampling effect or the combination of subpopulations (Aketarawong et al, 2006;Wang et al, 2003;Dai et al, 2004;Augustinos et al, 2005). Some samples failed to yield PCR products at loci Bd39, Bd43B, and Bd76 but they were successfully amplified at other loci, suggesting the existence of null alleles at these loci.…”

“…Nine (Bd1, Bd6, Bd19,Bd37, Bd39, Bd43A, Bd43B, Bd76 and Bd98) out of 17 novel microsatellite markers isolated from from B. dorsalis sensu stricto (Aketarawong et al, 2006) were used. PCR primers were synthesized by Invitrogen.…”

“…Microsatellite markers isolated from B. dorsalis sensu stricto (Aketarawong et al, 2006) have shown potential utility as population and species markers for the B. dorsalis complex.…”