Seven strains of mice as potential models of bovine pasteurellosis following intranasal challenge with a bovine pneumonic strain of Pasteurella multocida A:3; comparisons of disease and pathological outcomes

Hodgson, John; Dagleish, Mark P.; Gibbard, Louise; Bayne, C.W.; Finlayson, J.; Moon, G.M.; Nath, Mintu

doi:10.1016/j.rvsc.2013.01.015

Cited by 11 publications

(8 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…P. multocida is able to gain access to the lower respiratory tract and cause respiratory disease and systemic infection. In swine, P. multocida can cause a chronic polymicrobial infection (usually with Bordetella bronchiseptica ) called atrophic rhinitis ( 4 – 6 ). However, P. multocida is not considered part of the normal flora of birds, in which it can be a highly invasive primary pathogen ( 7 ).…”

Section: Introductionmentioning

confidence: 99%

Capsular Polysaccharide Interferes with Biofilm Formation byPasteurella multocidaSerogroup A

Petruzzi

Briggs

Tatum

et al. 2017

mBio

View full text Add to dashboard Cite

Pasteurella multocida is an important multihost animal and zoonotic pathogen that is capable of causing respiratory and multisystemic diseases, bacteremia, and bite wound infections. The glycosaminoglycan capsule of P. multocida is an essential virulence factor that protects the bacterium from host defenses. However, chronic infections (such as swine atrophic rhinitis and the carrier state in birds and other animals) may be associated with biofilm formation, which has not been characterized in P. multocida. Biofilm formation by clinical isolates was inversely related to capsule production and was confirmed with capsule-deficient mutants of highly encapsulated strains. Capsule-deficient mutants formed biofilms with a larger biomass that was thicker and smoother than the biofilm of encapsulated strains. Passage of a highly encapsulated, poor-biofilm-forming strain under conditions that favored biofilm formation resulted in the production of less capsular polysaccharide and a more robust biofilm, as did addition of hyaluronidase to the growth medium of all of the strains tested. The matrix material of the biofilm was composed predominately of a glycogen exopolysaccharide (EPS), as determined by gas chromatography-mass spectrometry, nuclear magnetic resonance, and enzymatic digestion. However, a putative glycogen synthesis locus was not differentially regulated when the bacteria were grown as a biofilm or planktonically, as determined by quantitative reverse transcriptase PCR. Therefore, the negatively charged capsule may interfere with biofilm formation by blocking adherence to a surface or by preventing the EPS matrix from encasing large numbers of bacterial cells. This is the first detailed description of biofilm formation and a glycogen EPS by P. multocida.

show abstract

Section: Introductionmentioning

confidence: 99%

Capsular Polysaccharide Interferes with Biofilm Formation byPasteurella multocidaSerogroup A

Petruzzi

Briggs

Tatum

et al. 2017

mBio

View full text Add to dashboard Cite

show abstract

“…It is well known that the natural infectious route for P. multocida serotype A strain is through intranasal infection, but we found that intranasal infection results in a poor reproducibility among mice. Moreover, there are some similarities between intranasal infection and intraperitoneal infection, including the pathological alterations and expressions of inflammatory cytokines (Praveena et al, 2010; Hodgson et al, 2013). Thus, intraperitoneal infection was used in this study.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis on Responses of Murine Lungs to Pasteurella multocida Infection

Qin

et al. 2017

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Pasteurella multocida infection in cattle causes serious epidemic diseases and leads to great economic losses in livestock industry; however, little is known about the interaction between host and P. multocida in the lungs. To explore a fully insight into the host responses in the lungs during P. multocida infection, a mouse model of Pasteurella pneumonia was established by intraperitoneal infection, and then transcriptomic analysis of infected lungs was performed. P. multocida localized and grew in murine lungs, and induced inflammation in the lungs, as well as mice death. With transcriptomic analysis, approximately 107 clean reads were acquired. 4236 differently expressed genes (DEGs) were detected during P. multocida infection, of which 1924 DEGs were up-regulated. By gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichments, 5,303 GO enrichments and 116 KEGG pathways were significantly enriched in the context of P. multocida infection. Interestingly, genes related to immune responses, such as pattern recognition receptors (PRRs), chemokines and inflammatory cytokines, were significantly up-regulated, suggesting the key roles of these genes in P. multocida infection. Transcriptomic data showed that IFN-γ/IL-17-related genes were increased, which were validated by qRT-PCR, ELISA, and immunoblotting. Our study characterized the transcriptomic profile of the lungs in mice upon Pasteurella infection, and our findings could provide valuable information with respect to better understanding the responses in mice during P. multocida infection.

show abstract

“…During the past years, it has been reported that mice are exquisitely susceptible to P. multocida (Dawkins et al, 1990;Hodgson et al, 2013;Wilson & Ho, 2013). The studies made several observations using P. multocida in different strains of mice and concluded that P. multocida could produce 100% mortality in mice.…”

mentioning

confidence: 99%

Clinical and histopathological study on reproductive lesions caused by Pasteurella multocida type B2 immunogens in buffalo heifers

Ibrahim¹,

Jesse²,

Abba³

et al. 2018

BJVM

View full text Add to dashboard Cite

Haemorrhagic septicaemia (HS) is a globally renowned disease that affects cattle and buffaloes. Its effects on the reproductive system have not been previously studied. The present study evaluated the pathological responses in pre-pubertal female buffaloes infected with immunogens; lipopolysaccharide (LPS) and outer membrane protein (OMP) derived from P. multocida type B:2. Fifteen healthy pre-pubertal female buffaloes were divided into 5 equal groups. Buffaloes in groups 1 and 2 were intravenously and orally inoculated with P. multocida LPS, while those in groups 3 and 4 were subcutaneously and orally inoculated with P. multocida OMP. Buffaloes in group 5 were inoculated with sterile phosphate buffered saline (negative control). During the post infection period, all buffaloes were examined for clinical signs throughout 21 days and surviving animals were euthanised for postmortem evaluation. Histopathological evaluation of buffaloes inoculated intravenously with LPS showed a significantly higher occurrence (P<0.05) of necrosis and degeneration in the uterine horn and supramammary glands . Following inoculation with OMP, haemorrhage and congestion was significantly higher (P<0.05) in the ovaries, cervix, vagina, mammary and supra-mammary glands of buffaloes inoculated subcutaneously, while necrosis and degeneration and inflammatory cell infiltration in different organs was also significantly increased in the same group. The findings showed that both P. multocida bacterial LPS and OMP affected the uterine horn, ovaries, cervix, mammary gland and supramammary lymph nodes of buffalo calves inoculated either intravenously or subcutaneously, as compared to those inoculated orally.

show abstract

Seven strains of mice as potential models of bovine pasteurellosis following intranasal challenge with a bovine pneumonic strain of Pasteurella multocida A:3; comparisons of disease and pathological outcomes

Cited by 11 publications

References 42 publications

Capsular Polysaccharide Interferes with Biofilm Formation byPasteurella multocidaSerogroup A

Capsular Polysaccharide Interferes with Biofilm Formation byPasteurella multocidaSerogroup A

Transcriptomic Analysis on Responses of Murine Lungs to Pasteurella multocida Infection

Clinical and histopathological study on reproductive lesions caused by Pasteurella multocida type B2 immunogens in buffalo heifers

Contact Info

Product

Resources

About

Seven strains of mice as potential models of bovine pasteurellosis following intranasal challenge with a bovine pneumonic strain of Pasteurella multocida A:3; comparisons of disease and pathological outcomes

Cited by 11 publications

References 42 publications

Capsular Polysaccharide Interferes with Biofilm Formation byPasteurella multocidaSerogroup A

Capsular Polysaccharide Interferes with Biofilm Formation byPasteurella multocidaSerogroup A

Transcriptomic Analysis on Responses of Murine Lungs to Pasteurella multocida Infection

Clinical and histopathological study on reproductive lesions caused by Pasteurella multocida type B2 immunogens in buffalo hei­fers

Contact Info

Product

Resources

About

Clinical and histopathological study on reproductive lesions caused by Pasteurella multocida type B2 immunogens in buffalo heifers