Seven new metabolites of drostanolone heptanoate by using Beauveria bassiana, and Macrophomina phaseolina cell suspension cultures

Abstract

Incubation of drostanolone heptanoate (1) with B. bassiana and M. phaseolina afforded seven new and two known metabolites.The main sites of hydroxylation include C-5, C-7, C-11, C-14, C-15, and C-20, hydrolysis at C-17, and reduction at C-3 of 1.

Structural transformation of methasterone with Cunninghamella blakesleeana and Macrophomina phaseolina

“…19,20 There are several studies on metabolism of methasterone (1) has been reported. 10,18,21,22 In our continuing studies of biotransformation on steroids, [23][24][25][26][27][28][29][30][31] previously biocatalysis of methasterone (1) was performed by our research group, which resulted into several new anti-inammatory compounds. 32 Based on the previous results of compound 1, biotransformation of 1 was performed again by using Macrophomina phaseolina and Cunninghamella blakesleeana, which have been demonstrated to be effective cell cultures for bioconversion of steroids.…”