In Southeastern Brazil, there is good cultivation potential for the pearl oyster Pinctada imbricata, which is often found on mussel ropes or seed collectors across the north coast of Sao Paulo State. Despite this, very few studies have focused on the biology of this species in Brazil. This research aimed to partially address this lack of information by evaluating the optimal season and preferred depth range (surface, first and second metres of depth) for the settlement of juvenile P. imbricata on artificial collectors. Two replicate artificial collectors made from braided fishing nets and comprising of horizontally and vertically suspended lines were deployed at Cocanha Beach, Brazil, and left in situ for a period of 5 months. At the end of the deployment period, the density of juveniles was significantly greater on the surface than the first and second metres of depth. It was also found that the period from November to March was more favourable for the placement of collectors and settlement of juveniles. Furthermore the results of the study confirm that is feasible to capture juvenile P. imbricata using artificial collectors to provide a continuous supply of commercial cultures.