Settlement and metamorphosis of Rapana venosa (Gastropoda: Muricidae) with implications for artificial culture

Yu, Zhenglin; Yang, Mei‐Jie; Song, Hao; Hu, Zhi; Zhou, Cong; Wang, Xiaolong; Li, Haizhou; Zhang, Tao

doi:10.1007/s00343-019-9107-8

Cited by 10 publications

(9 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our study, at the highest stocking density of 40 ind/ml, the hatching success rates of larvae under RAS and TA were 72.3% and 61% respectively. Similar results were reported for the whelk Rapana venosa (Yu et al, 2020) and the geoduck clam Panopea generosa (Marshall et al, 2014): when the stocking density of larvae exceeded a certain limit, the survival rate of larvae declined. When the stocking density of larvae of the nudibranch Hermissenda crassicornis was maintained at 0.5–4 ind/ml, survival rate did not differ significantly among the groups; at 15 ind/ml; however, the survival rate was significantly lower (Avila et al, 1997).…”

Section: Discussionsupporting

confidence: 81%

Effect of stocking density on the growth and settlement of Haliotis discus hannai larvae in a recirculating aquaculture system

Zhang

Gao

Lyu

et al. 2021

Aquaculture Research

View full text Add to dashboard Cite

Settlement and metamorphosis is the key stage during which Haliotis discus hannai larvae change from planktonic to benthic life. Larvae tend to be highly sensitive to changes in the external environment, which can result in increased mortality. In the aquaculture industry, increasing the metamorphosis rate of larvae is considered to be a critical factor for increasing yield. In this study, we modified a recirculating aquaculture system (RAS) for dense rearing of abalone larvae. We set up five density groups (1, 5, 10, 20 and 40 ind/ml) and compared the effects of traditional aquaculture (TA) and the RAS on the hatching success rate, metamorphosis rate and the expression levels of metamorphosis‐related genes. At stocking densities of 1, 5 and 10 ind/ml, the survival rate, metamorphosis rate and expression levels of MIRP, α2ADR and DopR2 in both culture modes were significantly higher than those in the 20 and 40 ind/ml densities (p < 0.05). The metamorphosis rate and survival rate of larvae in the RAS groups were significantly higher than those in the TA groups (p < 0.05). Under TA at the 40 ind/ml stocking density, the dissolved oxygen concentration in the water declined and the TAN concentration significantly increased (p < 0.05). As the stocking density increased, the size of metamorphosed larvae under each mode tended to increase in both culture modes. In particular, when the density was highest, the time required for metamorphosis was significantly extended (p < 0.05). Based these results, use of the RAS with stocking density of 10 ind/ml would maintain a stable aquaculture water environment, prevent fertilized eggs from hatching or becoming densely aggregated before larval metamorphosis, and significantly increase the stocking density per unit water. These results provide an important reference for scaling up reproduction of larval abalone and further developing the culture mode for this economically important shellfish.

show abstract

Section: Discussionsupporting

confidence: 81%

Effect of stocking density on the growth and settlement of Haliotis discus hannai larvae in a recirculating aquaculture system

Zhang

Gao

Lyu

et al. 2021

Aquaculture Research

View full text Add to dashboard Cite

show abstract

“…Our previous study found that oysters can significantly improve the metamorphosis rate of larvae, and juvenile oysters less than 3 cm in size had a better induction effect, especially the oysters which were just metamorphosis [15] ( Fig. S1 ).…”

Section: Introductionmentioning

confidence: 90%

Symbiotic microbiome and metabolism profiles reveal the effects of induction by oysters on the metamorphosis of the carnivorous gastropod Rapana venosa

Yang

Song

Feng

et al. 2022

Computational and Structural Biotechnology Journal

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, there is no further study on the regulation of the neuroendocrine system on the metamorphosis of R. venosa. Bather (1921) showed that the presence of conspecific adults or useful food sources could trigger metamorphosis in marine invertebrates, and our previous study suggested that oysters can significantly improve the metamorphosis rate of larvae (Yu et al, 2020). In this process, oysters were inducers, as well as food sources; these results may suggest a link between the digestive system and metamorphosis and that the neuroendocrine system regulates metamorphosis by regulating the digestive system, which is very likely to occur in carnivorous gastropods.…”

Section: Introductionmentioning

confidence: 75%

“…As a carnivorous gastropod, R. venosa needs to undergo a food habit transition from herbivorous to carnivorous during metamorphosis, which is different from the metamorphosis of lifelong herbivory gastropods and makes the mechanism of metamorphosis more complex. Our group has performed some studies on the metamorphosis of R. venosa, including studies of the differences in transcriptome, proteome, metabolome, digestive enzyme and microorganisms before and after metamorphosis (Song et al, 2016a,b,c;Yang et al, 2020a,b), as well as the effect of bait induction on the rate of metamorphosis (Yu et al, 2020). Song et al (2016a) mentioned that the expression of the NOS and 5-HT receptors decreased after metamorphosis (Song et al, 2016a).…”

Section: Introductionmentioning

confidence: 99%

The Characteristic of Critical Genes in Neuroendocrine System and Their Regulation on Food Habit Transition and Metamorphosis of Veined Rapa Whelk Rapana venosa (Valenciennes, 1846)

Yang

Feng²,

Song³

et al. 2021

Front. Mar. Sci.

Self Cite

View full text Add to dashboard Cite

Metamorphosis is a critical developmental event in mollusks, and neuroendocrine system plays an essential role in this process. Rapana venosa is an economically important shellfish in China, but the artificial technology of R. venosa aquaculture is limited by metamorphosis. As a carnivorous gastropod, food habit transition makes the mechanism of R. venosa metamorphosis more complex. To investigate the changes in the neuroendocrine system and to reveal its role in regulating the food habit transition and metamorphosis of R. venosa, we cloned the cDNA sequences encoding 5-hydroxytryptamine receptor (Rv-5HTR), nitric oxide synthetase (Rv-NOS) and cholecystokinin receptor (Rv-CCKR), and investigated their expression by quantitative real-time PCR analysis, and explore the spatio-temporal changes of 5-HT protein expression using Immunohistochemical (IHC) analysis. The expression of the three geens was significantly increased in the early intramembrane veliger stage, which indicates that the three genes are related to the development of digestive system. Additionally, expression of the three genes was decreased after metamorphosis, while Rv-NOS and Rv-CCKR were increasingly expressed in competent larvae, which may help the larvae find suitable environments and promote digestive system development for metamorphosis, and the result of 5-HT IHC analysis also reflects the development of neuroendocrine system. Furthermore, results show that CCK can effect the expression of digestive enzyme, NOS and 5-HT receptor. Finally, based on the present results, we hypothesized that CCK and CCK receptor may be critical regulatory factors of food habit transition and metamorphosis. These results might provide information on the development of neuroendocrine system of R. venosa, and new insight into the regulation of the food habit transition and metamorphosis of gastropods.

show abstract

Settlement and metamorphosis of Rapana venosa (Gastropoda: Muricidae) with implications for artificial culture

Cited by 10 publications

References 28 publications

Effect of stocking density on the growth and settlement of Haliotis discus hannai larvae in a recirculating aquaculture system

Effect of stocking density on the growth and settlement of Haliotis discus hannai larvae in a recirculating aquaculture system

Symbiotic microbiome and metabolism profiles reveal the effects of induction by oysters on the metamorphosis of the carnivorous gastropod Rapana venosa

The Characteristic of Critical Genes in Neuroendocrine System and Their Regulation on Food Habit Transition and Metamorphosis of Veined Rapa Whelk Rapana venosa (Valenciennes, 1846)

Contact Info

Product

Resources

About