Setting up for gastrulation in zebrafish

Marlow, Florence

doi:10.1016/bs.ctdb.2019.08.002

Cited by 18 publications

(13 citation statements)

References 255 publications

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“…Because zygotic Wnt/ß-catenin signaling has an opposite effect with respect to maternal Wnt/ß-catenin signaling and functions to specify the posterior region (Carron and Shi, 2016), these findings highlight the importance of maternal Dvl in setting up zygotic morphogenetic and patterning processes. They support the view that maternally expressed gene products perform essential functions after zygotic genome activation (Marlow, 2020;Solnica-Krezel, 2020). Thus, both maternal and zygotic Dvl dosages are important for proper cell movements and embryonic axis patterning that occur during gastrulation.…”

Section: Maternal Contributions Of Dvl Proteins To Axis Patterning Ansupporting

confidence: 70%

Decoding Dishevelled-Mediated Wnt Signaling in Vertebrate Early Development

Shi

2020

Front. Cell Dev. Biol.

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Dishevelled proteins are key players of Wnt signaling pathways. They transduce Wnt signals and perform cellular functions through distinct conserved domains. Due to the presence of multiple paralogs, the abundant accumulation of maternal transcripts, and the activation of distinct Wnt pathways, their regulatory roles during vertebrate early development and the mechanism by which they dictate the pathway specificity have been enigmatic and attracted much attention in the past decades. Extensive studies in different animal models have provided significant insights into the structure-function relationship of conserved Dishevelled domains in Wnt signaling and the implications of Dishevelled isoforms in early developmental processes. Notably, intra-and intermolecular interactions and Dishevelled dosage may be important in modulating the specificity of Wnt signaling. There are also distinct and redundant functions among Dishevelled isoforms in development and disease, which may result from differential spatiotemporal expression patterns and biochemical properties and post-translational modifications. This review presents the advances and perspectives in understanding Dishevelled-mediated Wnt signaling during gastrulation and neurulation in vertebrate early embryos.

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Section: Maternal Contributions Of Dvl Proteins To Axis Patterning Ansupporting

confidence: 70%

Decoding Dishevelled-Mediated Wnt Signaling in Vertebrate Early Development

Shi

2020

Front. Cell Dev. Biol.

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“…Gastrulation in particular, requires precise coordination of cell fate specification, wherein the cells of the epiblast simultaneously segregate and differentiate into the three primary germ layers: ectoderm, mesoderm, and endoderm. Gastrulation is stimulated by morphogens and growth factors, modulated by their respective inhibitors, and directed by transcriptional programs that are influenced by epigenetics (Marlow, 2019;Molè et al, 2020;Raffaelli and Stern, 2020;Stathopoulos and Newcomb, 2020). The morphogenesis that occurs during gastrulation relies on cell tension and tissue-level force to facilitate the physical segregation of epiblast cells into the primary germ layers (Hamada, 2015;Ko and Martin, 2020;Paré and Zallen, 2020;Voiculescu et al, 2014;Williams and Solnica-Krezel, 2017).…”

Section: Introductionmentioning

confidence: 99%

Mechanics regulate human embryonic stem cell self-organization to specify mesoderm

Muncie

Ayad

Lakins

et al. 2020

Preprint

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Embryogenesis is directed by morphogens that induce differentiation within a defined tissue geometry. Tissue organization is mediated by cell-cell and cell-extracellular matrix (ECM) adhesions and is modulated by cell tension and tissue-level force. Whether cell tension regulates development by directly influencing morphogen signaling remains unclear. Human embryonic stem cells (hESCs) exhibit an intrinsic capacity for self-organization that motivates their use as a tractable model of early human embryogenesis. We engineered patterned substrates that enhance cell-cell interactions to direct the self-organization of cultured hESCs into "gastrulation-like" nodes. Tissue geometries that generate local nodes of high cell-cell tension and induce these selforganized tissue nodes drive BMP4-dependent gastrulation by enhancing phosphorylation and nuclear translocation of β-catenin to promote Wnt signaling and mesoderm specification. The findings underscore the interplay between tissue organization, cell tension, and morphogendependent differentiation, and demonstrate that cell-and tissue-level forces directly regulate cell fate specification in early human development.

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“…Symmetry breaking, axis elongation and neural specification occur despite the absence of extraembryonic signaling (Fulton et al, 2020;Schauer et al, 2020). In fish, these events are controlled maternally (Marlow, 2020;Solnica-Krezel, 2020) and A. mexicanus with its two morphotypes has become an excellent model to challenge the role of maternal determinants in embryogenesis (Ma et al, 2018(Ma et al, , 2020Torres-Paz et al, 2019).…”

Section: Generation Of Astyanax Pescoidsmentioning

confidence: 99%

Pescoids and Chimeras to Probe Early Evo-Devo in the Fish Astyanax mexicanus

Torres-Paz

Rétaux

2021

Front. Cell Dev. Biol.

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The fish species Astyanax mexicanus with its sighted and blind eco-morphotypes has become an original model to challenge vertebrate developmental evolution. Recently, we demonstrated that phenotypic evolution can be impacted by early developmental events starting from the production of oocytes in the fish ovaries. A. mexicanus offers an amenable model to test the influence of maternal determinants on cell fate decisions during early development, yet the mechanisms by which the information contained in the eggs is translated into specific developmental programs remain obscure due to the lack of specific tools in this emergent model. Here we describe methods for the generation of pescoids from yolkless-blastoderm explants to test the influence of embryonic and extraembryonic tissues on cell fate decisions, as well as the production of chimeric embryos obtained by intermorph cell transplantations to probe cell autonomous or non-autonomous processes. We show that Astyanax pescoids have the potential to recapitulate the main ontogenetic events observed in intact embryos, including the internalization of mesodermal progenitors and eye development, as followed with zic:GFP reporter lines. In addition, intermorph cell grafts resulted in proper integration of exogenous cells into the embryonic tissues, with lineages becoming more restricted from mid-blastula to gastrula. The implementation of these approaches in A. mexicanus will bring new light on the cascades of events, from the maternal pre-patterning of the early embryo to the evolution of brain regionalization.

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Setting up for gastrulation in zebrafish

Cited by 18 publications

References 255 publications

Decoding Dishevelled-Mediated Wnt Signaling in Vertebrate Early Development

Decoding Dishevelled-Mediated Wnt Signaling in Vertebrate Early Development

Mechanics regulate human embryonic stem cell self-organization to specify mesoderm

Pescoids and Chimeras to Probe Early Evo-Devo in the Fish Astyanax mexicanus

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