Sesuvium portulacastrum (L.) L. a promising halophyte: cultivation, utilization and distribution in India

Plant Cell Tiss Organ Cult

Nikam

Suprasanna

2010

Self Cite

106

In vitro-grown cells of Sesuvium portulacastrum L., an important 'salt accumulator' mangrove associate, were incubated on a medium containing different levels of salt, including 0, 100, 200, or 400 mM NaCl, in order to evaluate biochemical, physiological, and growth responses. A significant decrease in callus growth, water status, and cell membrane damage was observed under salt stress. Osmotic adjustment was revealed by the accumulation of inorganic ions, such as sodium (Na ? ), and organic osmolytes (proline, glycine betaine, and total soluble sugars) in NaCl-treated calli compared to control. However, accretion of osmolytes and inorganic ions did not support growth of calli under NaCl stress conditions. The observed reduced growth rate in calli subjected to stress, up to 200 mM NaCl, was coupled with lower catalase and ascorbate peroxidase activities and with a significantly higher superoxide dismutase activity. These findings suggested that S. portulacastrum cell cultures exhibited higher osmotic adjustment to salt stress.

Section: Introductionmentioning

confidence: 99%

Biochemical, physiological and growth changes in response to salinity in callus cultures of Sesuvium portulacastrum L.

Plant Cell Tiss Organ Cult

Nikam

Suprasanna

2010

Self Cite

106

“…The Sesuvium clone-MH (Maharashtra) planted and maintained in the Botanic garden at the Department of Botany, University of Pune, India, (Lokhande et al 2009) was used as source plant material. The four nodal sectors (*4.0 cm) containing single pre-existing axillary bud with two opposite leaves were planted in the plastic pots (10 9 20 cm) containing sand: soil (1:1) mixture.…”

Section: Plant Materials and Treatment Conditionsmentioning

confidence: 99%

Regulated alterations in redox and energetic status are the key mediators of salinity tolerance in the halophyte Sesuvium portulacastrum (L.) L

Srivastava

et al. 2011

Plant Growth Regul

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The present work addresses the importance of antioxidant, redox and energetic parameters in regulating salt-tolerance in Sesuvium portulacastrum. Experiments were conducted on 45 days old plants subjected to 250 and 1,000 mM NaCl stress for 2-8 days. Plants showed no significant change in growth parameters (shoot length, dry weight, and water content) at 250 mM NaCl as compared to control. However, growth of plants was significantly affected at 1,000 mM NaCl. The differential growth behaviour could be attributed to a greater decline in the energetic parameters (in terms of ratios of NADP/NADPH and ATP/ADP) at 1,000 mM NaCl than at 250 mM NaCl. The osmotic stress imposed to plants at 250 mM NaCl was presumably balanced by the accumulation of sodium ions (Na ? ), an energetically favorable process, and did not require an increased synthesis of proline. In contrast, to counter osmotic stress at 1,000 mM NaCl, plants accumulated Na ? as well as proline and were, therefore, energetically stressed. Further, the response of enzymatic and molecular antioxidants at 1,000 mM was either close to or even lower than that at 250 mM, which resulted in oxidative damage at 1,000 mM, particularly on longer durations. In conclusion, it is suggested that altered redox and energetic status of the plants could play a key role in mediating the tolerance of Sesuvium under salinity stress.

“…This plant is used as a fodder for animals and has an ornamental value since it blooms throughout the year in the barren areas (Lokhande et al 2009a). Due to its survival in adverse environmental conditions, the plant is recognized as a promising candidate for the environmental protection (Ghnaya et al 2005(Ghnaya et al , 2007Lokhande et al 2009aLokhande et al , 2010aRabhi et al 2009Rabhi et al , 2010Moseki and Buru 2010;Zaier et al 2010a, b). The experiments have shown the optimum growth potential of the plant in the presence of salt concentrations of NaCl (200 mM) (Lokhande et al 2010b;Moseki and Buru 2010).…”

Section: Introductionmentioning

confidence: 99%

Sesuvium portulacastrum (L.) L.: a potential halophyte for the degradation of toxic textile dye, Green HE4B

Patil

Suprasanna

et al. 2011

Planta

Self Cite

Sesuvium portulacastrum is a common halophyte growing well in adverse surroundings and is exploited mainly for the environmental protection including phytoremediation, desalination and stabilization of contaminated soil. In the present investigation, attempts have been made on the decolorization of a toxic textile dye Green HE4B (GHE4B) using in vitro grown Sesuvium plantlets. The plantlets exhibited significant (70%) decolorization of GHE4B (50 mg l(-1)) that sustain 200 mM sodium chloride (NaCl) within 5 days of incubation. The enzymatic analysis performed on the root and shoot tissues of the in vitro plantlets subjected to GHE4B decolorization in the presence of 200 mM NaCl showed a noteworthy induction of tyrosinase, lignin peroxidase and NADH-DCIP reductase activities, indicating the involvement of these enzymes in the metabolism of the dye GHE4B. The UV-visible spectrophotometer, HPLC and Fourier Transform Infrared Spectroscopy (FTIR) analyses of the samples before and after decolorization of the dye confirmed the efficient phytotransformation of GHE4B in the presence of 200 mM NaCl. Gas Chromatography-Mass Spectroscopy (GC-MS) analysis of the products revealed the formation of three metabolites such as p -amino benzene, p -amino toluene and 1, 2, 7-amino naphthalene after phytotransformation of GHE4B. Based on the FTIR and GC-MS results, the possible pathway for the biodegradation of GHE4B in the presence of 200 mM NaCl has been proposed. The phytotoxicity experiments confirmed the non-toxicity of the degraded products. The present study demonstrates for the first time the potential of Sesuvium for the efficient degradation of textile dyes and its efficacy on saline soils contaminated with toxic compounds.