Sesterterpenoids, Terretonins A−D, and an Alkaloid, Asterrelenin, from Aspergillus terreus

Li, Guo‐You; Li, Bogang; Yang, Tao; Yin, Jianhua; Qi, Hua‐Yi; Liu, Guangye; Zhang, Guolin

doi:10.1021/np0501738

Cited by 72 publications

(51 citation statements)

References 8 publications

(13 reference statements)

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“…Antifungal activity of A. terreus st. 1 was first evaluated with disc diffusion assay and further confirmed by broth dilution test. A. terreus st. 1 was identified to have efficient anti-candidal activity probably due to its significant production of unique secondary metabolites which is evident as A. terreus strains are reported to produce unique bioactive compounds (Almassi et al, 1996;Li et al, 2005). The Candida albicans activity was not observed in all other the fungal species screened might be due to lack of anticandidal metabolites.…”

Section: Discussionmentioning

confidence: 99%

“…Fungi offer the advantage that most species produce bioactive compounds during growth and metabolism allowing tracing their mycelia and metabolites in their natural substrates and habitats (Kettering et al, 2004). Isolates of A. terreus obtained from terrestrial habitats have been known to produce variety of secondary metabolites with various biological activity including terretonins (Li et al, 2005), terrecyclic acids (Almassi et al, 1996) asterredione (Wijeratne et al, 2003). Bioassay guided isolation and identification of compounds are helpful to point out the specific bioactive compounds in a mixture of metabolites (Sule et al, 2011;Nalina and Rahim, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Bioautography Guided Identification of Anticandidal Compounds from A. terreus st. 1

Farouk¹

2011

American Journal of Infectious Diseases

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Problem statement: Saprophytic and soil inhabiting microorganisms are prolific producers of secondary metabolites with wide range of structural and functional diversity. The objective of the current study was to screen and evaluate the anti-candidal properties of soil inhabiting fungi. Approach: Preliminary dual culture assay and bioautography was used to screen the fungi with potential anti-fungal activity and direct detection of antifungal compound/fraction in microbial extract on thin layer chromatograms, respectively. Disk diffusion, broth dilution and germ tube inhibitory assay were used to evaluate and confirm the anticandidal activity. Partial characterization of purified extract was carried out using High Performance Liquid Chromatography (HPLC) and Fast Performance Liquid Chromatography (FPLC) techniques. Result and Discussion: A. terreus was found to produce compound with anti-candidal and germ tube inhibitory activity against human pathogen Candida albicans at 200 mg ml -1. Further proteinase inhibitory activity of the extract, tested using plate assay and SDS-PAGE ensures the anti-candidal activity of the extract. Present study identified and confirmed the potential anticandidal activity, interms of germ tube and proteinase inhibititory activity, of metabolites produced by A. terreus st.1. Conclusion: The bio-assay guided fractionation and purification resulted in identification of unique fraction with active anti-candidal activity. Thus this fraction may serve as one of the lead to develop novel anti-candidal therapeutics.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bioautography Guided Identification of Anticandidal Compounds from A. terreus st. 1

Farouk¹

2011

American Journal of Infectious Diseases

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“…As part of our research on marine fungi, we report here the results regarding the secondary metabolite chemistry of 2P-22 strain, Aspergillus sp., isolated from the marine sponge Cliona chilensis collected in Los Molles, IV Region, Chile. This study led to the isolation of a new compound 1, together with three known compounds previously isolated from other fungal sources [8][9][10][11] ( Figure 1). The new compound is structurally related to the known butyrolactone I isolated from Aspergillus terreus.…”

Section: Introductionmentioning

confidence: 92%

New Butyrolactone From a Marine-Derived Fungus Aspergillus Sp

San-Martı́n

Rovirosa

Vaca

et al. 2011

J. Chil. Chem. Soc.

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Four compounds that belong to two structure types, namely dibenzylbutyrolactone and sesterterpenoids, were obtained from the extract of the strain Aspergillus sp. (2P-22), isolated from a marine sponge, Cliona chilensis. Among them, compound 1 was identified as new, namely butylrolactone-VI. The structures of these compounds were characterized on the basis of spectroscopic data. Biological activities of these fungal metabolites, are described.

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“…4,5 The chromogenic substrate is hydrolyzed enzymatically to produce Ph(NO2), which renders the reaction mixture yellow. In this study, α-glucosidase was examined in 5 mM Ph(NO2)-Glc and 0.1 M NaH2PO4-Na2HPO4 (pH 7.0) with the different concentrations of the enzyme, cEnz.…”

Section: Enzyme Assaymentioning

confidence: 99%

“…4,5 If glucose can be determined in the reaction mixture, the enzyme reaction and the inhibition can be examined using a natural substrate, such as maltose or sucrose. In a previous paper, 6 we presented a colorimetric method for the determination of reducing monosaccharide.…”

Section: Introductionmentioning

confidence: 99%

Microplate Assay of α-Glucosidase and Its Inhibitors Based on the Direct Reduction of Molybdosilicate by Glucose

et al. 2015

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A colorimetric method for monosaccharide determination (Anal. Sci., 2013, 29, 1021 was optimized for the high-throughput screening of α-glucosidase, which hydrolyzes an α-1,4-glycosidic bond of starch and related oligo-and polysaccharides, followed by the release of D-glucose from the non-reducing ends. In a microplate, 40 μL of a sample solution was mixed with 160 μL of a 50 mM Na2SiO3, 600 mM Na2MoO4, 1.5 M CH3COOH, and 20% (v/v) dimethyl sulfoxide solution, which was yellowish due to the formation of a yellow molybdosilicate. The mixture was kept at 80 C for 60 min. In the mixture, glucose reduced the Mo(VI) species directly to form a blue heteropolymolybdate(V/VI). Thus, 0.1 mM level glucose can be determined by the color change from yellow to blue. Since maltose cannot render the mixture blue as strongly as glucose, the present method has been successfully applied to a microtiter plate assay of α-glucosidase with the disaccharide. Also, the method has been applied to an assay of α-glucosidase inhibitors, acarbose and quercetin.

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Sesterterpenoids, Terretonins A−D, and an Alkaloid, Asterrelenin, from Aspergillus terreus

Cited by 72 publications

References 8 publications

Bioautography Guided Identification of Anticandidal Compounds from <I>A. terreus</I> st. 1

Bioautography Guided Identification of Anticandidal Compounds from <I>A. terreus</I> st. 1

New Butyrolactone From a Marine-Derived Fungus Aspergillus Sp

Microplate Assay of α-Glucosidase and Its Inhibitors Based on the Direct Reduction of Molybdosilicate by Glucose

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