Sesquiterpene Lactones from Inula britannica and Their Cytotoxic and Apoptotic Effects on Human Cancer Cell Lines

Bai, Naisheng; Lai, Ching-Shu; He, Kan; Zhou, Zhu; Zhang, Li; Zheng, Qingdong; Zhu, Nanqun; Zheng, Qun Yi; Pan, Min‐Hsiung; Ho, Chi‐Tang

doi:10.1021/np050437q

Cited by 66 publications

(42 citation statements)

References 16 publications

(23 reference statements)

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“…Britannilactone (BL), 1-O-acetylbritannilactone (OABL), and 1,6-O,O-diacetylbritannilactone (OODBL) (Fig. 1), isolated from I. britannica, exhibit potent antitumor activities against several human cancer cell lines [4,5]. The antitumor properties of OODBL has been studied with respect to apoptosis and cell cycle arrest [4,6].…”

Section: Introductionmentioning

confidence: 99%

Involvement of MAPK, Bcl‐2 family, cytochrome c, and caspases in induction of apoptosis by 1,6‐O,O‐diacetylbritannilactone in human leukemia cells

Pan

Chiou

Cheng

et al. 2007

Molecular Nutrition Food Res

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1,6-O,O-diacetylbritannilactone (OODBL) isolated from Inula britannica, exhibits potent antitumor activity against several human cancer cell lines. However, the molecular mechanism of OODBL in the induction of anticancer activity is still unclear. In the present study, we demonstrated that OODBL induced the occurrence of apoptosis in human leukemic (HL-60) cells and cell arrest at the S phase. On the other hand, activation of caspase-8, -9, and -3, phosphorylation of Bcl-2 and Bid, and increased release of cytochrome c from mitochondria into cytosolic fraction were detected in OODBL-treated HL-60 cells. We further demonstrated that production of reactive oxygen species (ROS), activation of mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) signaling pathways may play an important role in OODBL-induced apoptosis. The results from the present study highlight the molecular mechanisms underlying OODBL-induced anticancer activity.

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Section: Introductionmentioning

confidence: 99%

Involvement of MAPK, Bcl‐2 family, cytochrome c, and caspases in induction of apoptosis by 1,6‐O,O‐diacetylbritannilactone in human leukemia cells

Pan

Chiou

Cheng

et al. 2007

Molecular Nutrition Food Res

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“…I. viscosa contains a large number of pharmacologically active compounds. Previous studies isolated and identified various chemical compounds in Inula species including flavonoids [29,30], terpenes [31], sesquiterpenoids [32][33] and sesquiterpene Lactones; [34]. We have investigated the traditional Palestinian I. viscosa plant crude extract in the past and found it to exhibit potential anti-malarial activity in vitro [35].…”

Section: Introductionmentioning

confidence: 99%

Pure Isolates and Preparative HPLC Fractions or Crude Extract of Inula viscosa: Effect on β-hematin Inhibition in vitro

Akkawi¹

2018

PPIJ

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The antimalarial activity of wild Inula viscosa (I. viscosa) plant leaves was investigated. The efficacy of the whole crude extract versus preparative HPLC fractions versus pure isolates were compared by measuring their effect on β-hematin inhibition in-vitro. The preparative HPLC experiments consisted of reversed phase preparative column (22.2mm x 250mm, 10μm) and linear gradient of water, acetonitrile as the mobile phase. Injection volume was 1000μl and the wavelengths range were from 200-450 nm using photodiode array detector (PDA). While fractions (IV, V and VI) showed antimalarial potential in comparison to chloroquine positive control, the rest of the fractions did not show any significant inhibition to the β-hematin formation. The antimalarial results showed that whole crude exact of the plant works better than the preparative fractions or the pure isolates presumably due to synergistic effect. The chemical identity of some of the pure isolates was explored using UHPLC-ESi-MS. Moreover, I. viscosa extract powder stayed stable over several years, while many other products such as Artemisia annua extract or Artemisin Combined Therapy (ACT) drugs rapidly lost their efficiency under tropical storage conditions. Keywords:

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“…Inula britannica is a commonly used Chinese traditional herbal medicines and its extracts have been reported to have immunomodulatory (11,12), anti-inflammatory (13) and antitumor activities (14)(15)(16). 1-O-acetylbritannilactone (ABL) is an effective chemical component extracted from inula britannica.…”

Section: Introductionmentioning

confidence: 99%

1-O-acetylbritannilactone combined with gemcitabine elicits growth inhibition and apoptosis in A549 human non-small cell lung cancer cells

Wang

Qiao

2015

Molecular Medicine Reports

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Abstract. Non-small-cell lung cancer (NSCLC) accounts for ~85% of all lung cancer cases, with a 5-year survival rate of <15%. 1-O-acetylbritannilactone (ABL), a natural chemical component obtained from inula britannica, a Chinese traditional medicine, has been demonstrated to have anticancer activity. In the present study, the antiproliferative and proapoptotic abilities of ABL alone or in combination with gemcitabine in a human NSCLC cell line were investigated. A549 cells were treated in vitro with ABL, gemcitabine, and a combination of ABL and gemcitabine for 72 h. The results demonstrated that ABL and gemcitabine inhibited cell growth and induced apoptosis of A549 cells. These effects were more potent following the combination of ABL and gemcitabine treatment than either agent alone. Furthermore, the signal transduction analysis revealed nuclear factor (NF)-κB expression was significantly decreased by ABL and the combination treatment. The inhibitor nuclear factor κBα (IκBα) and Bax levels were upregulated whereas Bcl-2 was substantially downregulated following treatment. The present findings suggest that ABL combined with gemcitabine elicits potent apoptosis of lung cancer cells and therefore, ABL has the potential to be developed as a chemotherapeutic agent.

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Sesquiterpene Lactones from Inula britannica and Their Cytotoxic and Apoptotic Effects on Human Cancer Cell Lines

Cited by 66 publications

References 16 publications

Involvement of MAPK, Bcl‐2 family, cytochrome c, and caspases in induction of apoptosis by 1,6‐O,O‐diacetylbritannilactone in human leukemia cells

Involvement of MAPK, Bcl‐2 family, cytochrome c, and caspases in induction of apoptosis by 1,6‐O,O‐diacetylbritannilactone in human leukemia cells

Pure Isolates and Preparative HPLC Fractions or Crude Extract of Inula viscosa: Effect on β-hematin Inhibition in vitro

1-O-acetylbritannilactone combined with gemcitabine elicits growth inhibition and apoptosis in A549 human non-small cell lung cancer cells

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