Sesquiterpene Lactone Content in Leaves ofin vitroand Field CultivatedArnica montana*

Abstract

On the basis of GC and GC/MS analyses we report on the full qualitative and quantitative sesquiterpene lactone (STL) content of in vitro cultivated A. montana plantlets consisting of helenalin and 11alpha,13-dihydrohelenalin esters in approximately equal amounts. The accumulation of STL was shown to be correlated with tissue differentiation in the above-ground parts. The seasonal variation of STL content in leaves of A. montana cultivated in the proving field was investigated. Changes in the composition of the STL fraction were detected. While young plants accumulate mainly helenalin derivatives, the content of such compounds decreases to almost zero within about 6 weeks from the beginning of leaf formation while that of dihydrohelenalin type compounds increases at the same rate and remains constant for a longer period.

Terpenoids from a multiple shoot culture of Telekia speciosa

“…2). Pseudoguaianolides were also produced by multiple shoots [23] and in vitro grown plantlets of Arnica montana [23,24]. Multiple shoots cultivated on a solidified medium and plantlets grown in a liquid medium as well as in vitro cultivated rooted plantlets on solidified media, accumulated the same set of sesquiterpene lactones: helenalin, 11α,13-dihydrohelenalin and their esters.…”

Plantas medicinais: fatores de influência no conteúdo de metabólitos secundários

“…São relatadas, por ex., variações sazonais no conteúdo de praticamente todas as classes de metabólitos secundários, como óleos essenciais [11][12][13][14][15] , lactonas sesquiterpênicas 16,17 , ácidos fenólicos 16,18 , flavonóides [19][20][21][22][23][24][25] , cumarinas 21 , saponinas [26][27] , alcalóides 1,[28][29][30][31] , taninos [32][33][34][35][36] , graxas epicuticulares 37 , iridóides 22,38,39 , glucosinolatos 40,41 e glicosídeos cianogênicos 42,43 .…”

“…É o caso, por ex., das lactonas sesquiterpênicas produzidas em Arnica montana, consideradas os principais princípios ativos desta planta utilizada como antiinflamatório; enquanto plantas jovens acumulam majoritariamente derivados da helenalina (Figura 3d), a concentração destes compostos é reduzida para praticamente zero após aproximadamente seis semanas contadas a partir da formação das folhas; por outro lado, os níveis de compostos do tipo diidrohelenalina aumentam muito e então se mantêm constantes por um longo período 17 . Situação semelhante ocorre com os metabólitos de Gentiana lutea, cujas folhas são ricas em Cglicosídeos, como a mangiferina (Figura 3b), na fase de floração, enquanto O-glicosídeos, como a isoorientina (Figura 3a), são acumulados principalmente antes do desenvolvimento das flores 22 .…”

Quantitative HPLC analysis of sesquiterpene lactones and determination of chemotypes in Eremanthus seidelii MacLeish & Schumacher (Asteraceae)

“…[3][4][5][6][7][8][9] Some of the SL isolated from the Eremanthus genus posses important biological activities, 3,[6][7][8]10 like the antiproliferative effects on some cancer cell lines 11 and the modulation of the inflammatory process in vitro, by inhibiting the NF-κβ transcriptor factor. 12 Seasonal, [13][14][15][16] circadian [17][18][19] and populational [20][21][22][23][24] variations in the content of almost all classes of secondary metabolites, including SL, [25][26][27][28][29] were reported. The recognition of variations in secondary metabolite production can be helpful in chemotaxonomic studies, specially for the determination of qualitative and quantitative chemical markers that could be applied to establish chemotypes and as additional taxonomic characters for the definition of complex taxa.…”