Sesquiterpene Lactams and Lactones With Antioxidant Potentials From Atractylodes macrocephala Discovered by Molecular Networking Strategy

Wang, Pan; Zhao, Yi-nan; Xu, Rui-Zhu; Zhang, Xiaowei; Sun, Yiran; Qi, Feng; Li, Zhonghua; Xu, Jiangyan; Xie, Zhishen; Zhang, Zhenqiang; Hengchao, E

doi:10.3389/fnut.2022.865257

Cited by 7 publications

(9 citation statements)

References 26 publications

(35 reference statements)

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“…AMP is widely used for its antioxidant, anti-inflammatory, anti-tumor, immunopotentiator, and intestinal health maintenance benefits [ 8 , 9 , 11 , 12 , 13 , 14 , 15 ]. In the present study, 400 mg/kg AMP significantly promoted WGR, SGR, and FE.…”

Section: Discussionmentioning

confidence: 99%

“…AMP can reduce inflammatory damage and oxidative stress via the NF-κB signaling pathway [ 11 , 12 ]. AMP can also enhance antioxidant defense capacity by promoting the expression of glutathione peroxidase (GPx) and SOD [ 13 , 14 ], which are related to the Nrf2 signaling pathway [ 15 ]. However, few studies report the effects of AMP on aquatic animals.…”

Section: Introductionmentioning

confidence: 99%

“…Dietary sodium butyrate can improve intestinal morphology by increasing the villus width and villus height [ 26 ]. Because AMP can promote growth, antioxidant activity, and disease resistance [ 8 , 9 , 11 , 12 , 13 , 14 , 15 ], it could be used as a feed additive in largemouth bass culture. The optimal supplemental level of AMP as a feed additive for largemouth bass was determined to be 400 mg/kg without negative effects (data unpublished).…”

Section: Introductionmentioning

confidence: 99%

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Tolerance Assessment of Atractylodes macrocephala Polysaccharide in the Diet of Largemouth Bass (Micropterus salmoides)

Dong

Chen

et al. 2022

Antioxidants

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Atractylodes macrocephala polysaccharide (AMP) can enhance antioxidant defense and anti-inflammation, as the tolerance levels of AMP in aquaculture is important for additive utilization. However, the tolerance dose of AMP is unknown. We assess the tolerance levels of AMP in juvenile largemouth bass (3.38 ± 0.11 g) by feeding them a 0, 400, 4000, or 8000 mg/kg AMP supplemented diet for 10 weeks. The 400 mg/kg AMP dose increased growth performance. The Nrf2/Keap1 signaling pathway was activated, as indicated by Keap1 and Nrf2 protein levels in the liver. Enhanced activity of antioxidant enzymes (SOD, GPx), together with increased mRNA levels of antioxidant genes (sod, gpx) and decreased accumulation of reactive oxygen species (ROS) and MDA, was found in the liver, implying the antioxidant effect of AMP. Nutrient absorption was enhanced by AMP, as reflected by the increased length of intestinal villi and microvilli. However, 4000 and 8000 mg/kg AMP induced oxidant stress, as indicated by increased plasma ALT and AST content and decreased mRNA levels of antioxidant genes (sod, gpx) in the liver and intestinal tissues. Inflammatory reactions were also induced by high doses of AMP, as reflected by enhanced levels of pro-inflammatory cytokines (tnfα, nfκb) in the liver, intestinal, and kidney tissues and inhibited levels of anti-inflammatory cytokines (tgfβ, iκb). Histological analysis reveals inflammatory cell infiltration and tissue damage. Thus, the safe tolerance margin of AMP supplement for largemouth bass was 400–4000 mg/kg.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tolerance Assessment of Atractylodes macrocephala Polysaccharide in the Diet of Largemouth Bass (Micropterus salmoides)

Dong

Chen

et al. 2022

Antioxidants

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“…According to previous studies, sesquiterpene lactams and lactones are the main active components of Atractylodes macrocephala , which perform excellent anti-inflammatory and antioxidant activities ( Bailly, 2021b ). Atractylodes are commonly used to heal gastrointestinal disorders due to their potential role in regulating intestinal microbiota ( Feng et al, 2020 ; Wang et al, 2022 ). Previous studies have indicated that several herbal medicines are usually combined to achieve the synergetic effect better than single herb based on their similar therapeutic properties ( Xu et al, 2022 ; Zhou et al, 2016 ; Li et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

The effect of combined dietary supplementation of herbal additives on carcass traits, meat quality, immunity and cecal microbiota composition in Hungarian white geese

Zhou

Song

et al. 2023

PeerJ

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The present study was performed to investigate the effects of dietary supplementation with herbal additives on meat quality, slaughter performance and the cecal microbial community in Hungarian white geese. A total of 60 newborn geese were assigned equally into the control group (CON) and the herbal complex supplemented group (HS). The dietary supplementations consisted of Compound Herbal Additive A (CHAA) including Pulsatilla, Gentian and Rhizoma coptidis, and Compound Herbal Additive B (CHAB) containing Codonopsis pilosula, Atractylodes, Poria cocos and Licorice. The geese in the HS group received a basal diet supplemented with 0.2% CHAA from day 0 to day 42 at the postnatal stage. Then from day 43 to day 70, the geese in HS group were provide a basal diet with 0.15% CHAB. The geese in the CON group were only provided with the basal diet. The results showed that the slaughter rate (SR), half chamber rates (HCR), eviscerated rate (ER) and breast muscle rate (BMR) in the HS group tended to increase slightly compared with the CON group (ns). In addition, the shear force, filtration rate and pH value of breast muscle and thigh muscle in the HS group were slightly enhanced compared to the CON group (ns). Significant increased levels in carbohydrate content, fat content and energy (P < 0.01) and significant decreased levels in cholesterol content (P < 0.01) were observed in the muscle of the HS group. The total amino acid (Glu, Lys, Thr and Asp) content in the muscle increased in HS group than in the CON group (P < 0.01). Dietary herb supplementations significantly increased the levels of IgG in serum (P < 0.05) on day 43 and higher levels of IgM, IgA and IgG (P < 0.01) were also observed in the HS group on day 70. Furthermore, 16S rRNA sequencing results indicated that herbal additives increased the growth of beneficial bacteria and inhibited the proliferation of harmful bacteria in the geese caecum. Altogether, these results offer crucial insights into the potential benefits of incorporating CHAA and CHAB into the diets of Hungarian white goose. The findings indicate that such supplementations could significantly improve meat quality, regulate the immune system and shape the intestinal microbiota composition.

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“…In recent years, immunoregulatory polysaccharides are considered important macromolecules for stimulation of immune response, then gradually become a major research hot spot (1,2). Atractylodes macrocephala Koidz (A. macrocephala) has been used both as traditional medicine and functional food for hundreds of years in Asia, and it was approved as a functional food by the National Health Commission of the People's Republic Health of China (3)(4)(5). Polysaccharide from A. macrocephala (AMP) has a variety of biological activities, such as enhancing the ability of immunity, modulating effect on gastrointestinal motility and decreasing the blood glucose level (6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

Polysaccharide from Atractylodes macrocephala Koidz binding with zinc oxide nanoparticles: Characterization, immunological effect and mechanism

Liu

Wang

et al. 2022

Front. Nutr.

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Atractylodes macrocephala Koidz (A. macrocephala) has been used both as a traditional medicine and functional food for hundreds of years in Asia. And it has a variety of biological activities, such as enhancing the ability of immunity and modulating effect on gastrointestinal motility. In this study, a watersoluble polysaccharide with molecular weight of 2.743 × 10 3 Da was isolated from the root of A. macrocephala. Polysaccharide from A. macrocephala (AMP) consisted of arabinose, galactose, glucose, xylose, mannose, ribose, galactose uronic acid, glucose uronic acid, with a percentage ratio of 21.86, 12.28, 34.19, 0.43, 0.92, 0.85, 28.79, and 0.67%, respectively. Zinc plays an important role in immune system. Therefore, we supposed that AMP binding with zinc oxide (ZnO) nanoparticles (AMP-ZnONPs) might be an effective immunostimulator. AMP-ZnONPs was prepared by Borch reduction, and its structural features were characterized by Scanning Electron Microscope (SEM), Transmission electron microscope (TEM), TEM-energy dispersive spectroscopy mapping (TEM-EDS mapping), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectrometer (XPS), X-ray diffraction (XRD), particle size and zeta-potential distribution analysis. Then, its immunostimulatory activity and the underlying mechanism were evaluated using RAW264.7 cells. The results showed that AMP-ZnONPs remarkably promoted cell proliferation, enhanced phagocytosis, the release of nitric oxide (NO), cytokines (IL-6 and IL-1β) and the expression of co-stimulatory molecules (CD80, CD86 and MHCII). Moreover, AMP-ZnONPs could promote Frontiers in Nutrition 01 frontiersin.org Bo et al. 10.3389/fnut.2022.992502the expression of Toll-like receptor 4 (TLR4), Myeloid differentiation factor 88 (MyD88), TNF receptor associated factor 6 (TRAF6), phospho-IκBα (P-IκBα) and phospho-p65 (P-p65), and TLR4 inhibitor (TAK242) inhibited the expression of these proteins induced by AMP-ZnONPs. Therefore, AMP-ZnONPs activated macrophages by TLR4/MyD88/NF-κB signaling pathway, indicating that AMP-ZnONPs could act as a potential immunostimulator in medicine and functional food. KEYWORDSpolysaccharide from Atractylodes macrocephala Koidz, zinc oxide nanoparticles, immunostimulatory activity, TLR4 signaling pathways, potential immunostimulator Materials and methods Reagents and materialsA. macrocephala was purchased from the Tongrentang Company in Beijing. The plant material was identified by Prof. Jingui Li. The purified AMP was prepared in our laboratory and the polysaccharide content was 96% (UV). RAW264.7 cells were obtained from American Type Culture Collection (ATCC,

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Sesquiterpene Lactams and Lactones With Antioxidant Potentials From Atractylodes macrocephala Discovered by Molecular Networking Strategy

Cited by 7 publications

References 26 publications

Tolerance Assessment of Atractylodes macrocephala Polysaccharide in the Diet of Largemouth Bass (Micropterus salmoides)

Tolerance Assessment of Atractylodes macrocephala Polysaccharide in the Diet of Largemouth Bass (Micropterus salmoides)

The effect of combined dietary supplementation of herbal additives on carcass traits, meat quality, immunity and cecal microbiota composition in Hungarian white geese

Polysaccharide from Atractylodes macrocephala Koidz binding with zinc oxide nanoparticles: Characterization, immunological effect and mechanism

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