Sesamol Inhibited Melanogenesis by Regulating Melanin-Related Signal Transduction in B16F10 Cells

Wu, Po-Yuan; You, Ying-Shu; Liu, Yi-Jung; Hou, Chien-Wei; Wu, Chin-Sheng; Wen, Kuo‐Ching; Lin, Chang-Shen; Chiang, Hsiu-Mei

doi:10.3390/ijms19041108

Cited by 45 publications

(40 citation statements)

References 35 publications

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“…Melanin overproduction was found to stimulate the cellular tyrosinase [ 35 ]. For this reason, reduction of tyrosinase activity is an efficient strategy in development of anti-melanogenic agents [ 36 , 37 ]. l -Tyrosine and l -DOPA are sequentially generated substrates that positively regulate melanogenesis and modulates melanocyte function through overlapping substrates [ 38 ].…”

Section: Resultsmentioning

confidence: 99%

A Potent Tyrosinase Inhibitor, (E)-3-(2,4-Dihydroxyphenyl)-1-(thiophen-2-yl)prop-2-en-1-one, with Anti-Melanogenesis Properties in α-MSH and IBMX-Induced B16F10 Melanoma Cells

et al. 2018

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In this study, we designed and synthesized eight thiophene chalcone derivatives (1a–h) as tyrosinase inhibitors and evaluated their mushroom tyrosinase inhibitory activities. Of these eight compounds, (E)-3-(2,4-dihydroxyphenyl)-1-(thiophen-2-yl)prop-2-en-1-one (1c) showed strong competitive inhibition activity against mushroom tyrosinase with IC50 values of 0.013 μM for tyrosine hydroxylase and 0.93 μM for dopa oxidase. In addition, we used enzyme kinetics study and docking program to further evaluate the inhibitory mechanism of 1c toward tyrosinase. As an underlying mechanism of 1c mediated anti-melanogenic effect, we investigated the inhibitory activity against melanin contents and cellular tyrosinase in B16F10 melanoma cells. As the results, the enzyme kinetics and docking results supports that 1c highly interacts with tyrosinase residues in the tyrosinase active site and it can directly inhibit tyrosinase as competitive inhibitor. In addition, 1c exhibited dose-dependent inhibitory effects in melanin contents and intracellular tyrosinase on α-MSH and IBMX-induced B16F10 cells. Overall, our results suggested that 1c might be considered potent tyrosinase inhibitor for use in the development of therapeutic agents for diseases associated with hyperpigment disorders.

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Section: Resultsmentioning

confidence: 99%

A Potent Tyrosinase Inhibitor, (E)-3-(2,4-Dihydroxyphenyl)-1-(thiophen-2-yl)prop-2-en-1-one, with Anti-Melanogenesis Properties in α-MSH and IBMX-Induced B16F10 Melanoma Cells

et al. 2018

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“…The distribution of melanin in the skin is completed by melanosome transport to the cell membrane and transfer to neighboring keratinocytes (Beaumont et al, 2011;Wu et al, 2018). While KIF5b contributes to outward melanosome transport along microtubules (Hara et al, 2000;Hirokawa et al, 2009), Rab27A-melanophilin-myosin Va complexes regulate melanosome transport along actin filaments (Raposo and Marks, 2007).…”

Section: Ppix Regulates Melanosome Transportmentioning

confidence: 99%

“…Melanin biosynthesis occurs in melanocytes, and its storage is restricted to melanosomes, which are melanocyte-specific lysosome-related organelles (Marks and Seabra, 2001). The mature melanosomes migrate along the microtubules and actin filaments from the perinuclear area to the cell membrane and are eventually transferred to neighboring keratinocytes (Beaumont et al, 2011;Wu et al, 2018;Makbal et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Protoporphyrin IX Stimulates Melanogenesis, Melanocyte Dendricity, and Melanosome Transport Through the cGMP/PKG Pathway

An²,

Jiang

et al. 2020

Front. Pharmacol.

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Protoporphyrin IX (PPIX) is a heterocyclic organic compound that is the last intermediate in the heme biosynthetic pathway. PPIX, due to its photodynamic effects, is utilized in the treatment of skin diseases. Furthermore, PPIX has been utilized as a melanogenesisstimulating agent in various studies. However, the exact function and mechanism underlying PPIX action in melanocytes remain to be elucidated. In the present study, we sought to further investigate how PPIX affects melanocyte melanogenesis, and whether PPIX is involved in melanin transport. Our findings demonstrated that PPIX increased melanocyte dendricity and melanosome transport, in addition to increasing melanogenesis. PPIX functions primarily by activating the guanylate cyclase (GC) and cyclic guanosine 3', 5'-monophosphate/protein kinase G (cGMP/PKG) signaling pathways. Once activated, these pathways increase tyrosinase activity and the expression of microphthalmiaassociated transcription factor (MITF), tyrosinase, tyrosinase-related protein-1 and-2 (TRP-1 and TRP-2), myosin Va, melanophinin, Ras-related protein Rab-27A (Rab27a), and cell division cycle 42 (Cdc42), promoting melanogenesis, melanocyte dendricity, and melanosome transport. Furthermore, the melanogenic effects of PPIX were confirmed in vivo in a zebrafish model system. Our results indicate that PPIX is not cytotoxic and may, thus, be utilized as a pigmentation enhancer.

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“…Melanin performs a significant part in defending human skin from the damaging impacts of radiation from UV sunlight and in scavenging toxic drugs and chemicals. However, overproduction of melanin may result in skin disorders such as melasma, solar lentigo, and postinflammatory hyperpigmentation, in relation to changing the shape of the skin …”

Section: Insight Of Molecular Mechanism Involve In Melanin Productionmentioning

confidence: 99%

“…However, overproduction of melanin may result in skin disorders such as melasma, solar lentigo, and postinflammatory hyperpigmentation, in relation to changing the shape of the skin. 24 Visible skin, hair, and eye pigmentation are primarily based on the different functions of melanocytes, a tiny subset of cells specializing in the synthesis and distribution of pigmented biopolymer melanin. Melanin is the main skin, hair, and eye color determinant.…”

Section: In S I G Ht Of Molecul Ar Mechanis M Involve In Mel Anin Pmentioning

confidence: 99%

Sjogren‐Larsson syndrome associated hypermelanosis

Hou

Zhu

et al. 2019

J of Cosmetic Dermatology

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Background/Objectives Sjogren – Larsson syndrome (SLS) is a rare autosomal recessive disease of the mutation ALDH3A2 that identifies a part of fatty acids for fatty aldehyde dehydrogenase: NAD‐oxidoreductase enzyme complex. This study aimed to access variant ALDH3A2 gene coded for FALDH and products regulating pathogenic melanogenesis owing to increased oxidative stress and reactive oxygen species resulting in DNA harm in SLS. By turning them into fatty acids, FALDH avoids the accumulation of toxic fatty aldehydes. The mutation results in the accumulation of aldehyde‐modified lipids or fatty alcohols that may interfere with skin and brain function. Methods In Nov 2018, we performed a literature search in PubMed for clinical studies, clinical trials, case reports, controlled trials, randomized controlled trials, and systemic reviews. The search terms we used were “SJOGREN‐LARSSON SYNDROME” AND “HYPERMELANNOSIS” OR “FALDH” (from 1985). The search resulted in 1,289 articles, out of these 95 articles met our inclusion exclusion criteria. Our inclusion criteria included relevant original articles relevant, critical systemic reviews, and crucial referenced articles, ex‐clusion criteria included duplicates and articles not published in English language. Results Toxicity of long‐chain aldehydes to FALDH‐deficient cells owing to accumulation under the profound epidermis layer improves oxidative stress in the cell resulting in keratinocyte hyperproliferation. Conclusion While it continues to be determined whether accumulated fatty alcohol and fatty aldehydes obtained from ether glycerolipids and sphingolipids improve the susceptibility of melanocytes and their element accountable for skin hyperpigmentation to biological colour.

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Sesamol Inhibited Melanogenesis by Regulating Melanin-Related Signal Transduction in B16F10 Cells

Cited by 45 publications

References 35 publications

A Potent Tyrosinase Inhibitor, (E)-3-(2,4-Dihydroxyphenyl)-1-(thiophen-2-yl)prop-2-en-1-one, with Anti-Melanogenesis Properties in α-MSH and IBMX-Induced B16F10 Melanoma Cells

A Potent Tyrosinase Inhibitor, (E)-3-(2,4-Dihydroxyphenyl)-1-(thiophen-2-yl)prop-2-en-1-one, with Anti-Melanogenesis Properties in α-MSH and IBMX-Induced B16F10 Melanoma Cells

Protoporphyrin IX Stimulates Melanogenesis, Melanocyte Dendricity, and Melanosome Transport Through the cGMP/PKG Pathway

Sjogren‐Larsson syndrome associated hypermelanosis

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