Sesamol ameliorates diet‐induced obesity in C57BL/6J mice and suppresses adipogenesis in 3T3‐L1 cells via regulating mitochondria‐lipid metabolism

Liu, Zhigang; Qiao, Qinglian; Sun, Yali; Chen, Yuwei; Ren, Bo; Liu, Xuebo

doi:10.1002/mnfr.201600717

Cited by 60 publications

(41 citation statements)

References 45 publications

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“…Our lab has shown sesamol possessed cytotoxicity in hepatocellular carcinoma cells in a previous publication18. There have been numerous of reports in the literature that sesamol possesses curative role in various diseases due to its diverse bioactivities in vivo such as anti-inflammatory, lipid-lowering, neuroprotective, and chemopreventive19353637. Previous study have demonstrated that oral administration of sesamol was absorbed and metabolized in the liver, which are then excreted in the bile or urine.…”

Section: Discussionmentioning

confidence: 88%

Sesamol Induces Human Hepatocellular Carcinoma Cells Apoptosis by Impairing Mitochondrial Function and Suppressing Autophagy

Liu

Ren

Wang

et al. 2017

Sci Rep

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Sesamol, a nutritional phenolic antioxidant compound enriched in sesame seeds, has been shown to have potential anticancer activities. This study aims at characterizing the antitumor efficacy of sesamol and unveiling the importance of mitochondria in sesamol-induced effects using a human hepatocellular carcinoma cell line, HepG2 cells. Results of this study showed that sesamol treatment suppressed colony formation, elicited S phase arrest during cell cycle progression, and induced both intrinsic and extrinsic apoptotic pathway in vitro with a dose-dependent manner. Furthermore, sesamol treatment elicited mitochondrial dysfunction by inducing a loss of mitochondrial membrane potential. Impaired mitochondria and accumulated H2O2 production resulted in disturbance of redox-sensitive signaling including Akt and MAPKs pathways. Mitochondrial biogenesis was inhibited as suggested by the decline in expression of mitochondrial complex I subunit ND1, and the upstream AMPK/PGC1α signals. Importantly, sesamol inhibited mitophagy and autophagy through impeding the PI3K Class III/Belin-1 pathway. Autophagy stimulator rapamycin reversed sesamol-induced apoptosis and mitochondrial respiration disorders. Moreover, it was also shown that sesamol has potent anti-hepatoma activity in a xenograft nude mice model. These data suggest that mitochondria play an essential role in sesamol-induced HepG2 cells death, and further research targeting mitochondria will provide more chemotherapeutic opportunities.

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Section: Discussionmentioning

confidence: 88%

Sesamol Induces Human Hepatocellular Carcinoma Cells Apoptosis by Impairing Mitochondrial Function and Suppressing Autophagy

Liu

Ren

Wang

et al. 2017

Sci Rep

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“…Cell lysates were solubilized in SDS sample buffer as previously described . Samples were separated by LaemmLi SDS/PAGE, and transferred onto PVDF membranes (Millipore, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…Cell lysates were solubilized in SDS sample buffer as previously described. [30] Samples were separated by LaemmLi SDS/PAGE, and transferred onto PVDF membranes (Millipore, Germany). Using appropriate antibodies, the immunoreactive bands were visualized with an enhanced chemiluminescence reagent.…”

Section: Western Blot Analysismentioning

confidence: 99%

(‐)‐Epigallocatechin‐3‐gallate Ameliorates Insulin Resistance and Mitochondrial Dysfunction in HepG2 Cells: Involvement of Bmal1

Gao

et al. 2017

Molecular Nutrition Food Res

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“…Previous studies have demonstrated that Sesamol, a liposoluble lignan extraction and prominent fragrance component in sesame oil, can improve PGC-1α genes expressions [24], which can activates Sirt3 gene promoter, leading to increase synthesis of Sirt3 mRNA transcripts [25]. Moreover, another finding suggested that Sirt3 is downregulated during severe hypertrophy and heart failure, and the overexpression of Sirt3 blocks cardiac hypertrophy by enhancing the activities of antioxidants SOD2, which thereby reduce the cellular ROS levels [26].…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies show that that Sirt3 is capable of blocking cardiac hypertrophy by reducing cellular ROS levels [26]. Another studies have demonstrated that Sesamol, a liposoluble lignan extraction and prominent fragrance component in sesame oil, can improve PGC-1α genes expressions [24]. Moreover, Sirt3 functions as a downstream target gene of PGC-1α and mediates the PGC-1α effects on cellular ROS production and mitochondrial biogenesis [44].…”

Section: Discussionmentioning

confidence: 99%

Sesamin Protects Against Cardiac Remodeling Via Sirt3/ROS Pathway

Fan

Zheng

Liu

et al. 2017

Cell Physiol Biochem

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Background/Aims: Cardiac remodeling is associated with oxidative stress. Sesamin, a well-known antioxidant from sesamin seeds, have been used extensively as traditional health foods. However, there is little known about the effect of sesamin on cardiac remodeling. Therefore, the present study aimed to determine whether sesamin could protect against cardiac remodeling and to clarify potential molecular mechanisms. Methods: The mice were subjected to either transverse aortic constriction (TAC) or sham surgery (control group). Beginning one week after surgery, the mice were oral gavage treated with sesamin (100mg·kg^-1·day^-1) or vehicle for 3 weeks. Cardiac hypertrophy was assessed by echocardiographic parameters, histological analyses and hypertrophic markers. Results: Sesamin alleviated cardiac hypertrophy, inhibited fibrosis and attenuated the inflammatory response. The increased production of reactive oxygen species, the activation of ERK1/2-dependent nuclear factor-κB and the increased level of Smad2 phosphorylation were observed in cardiac remolding model that were treated with sesamin. Furthermore, TAC induced alteration of Sirt3 and SOD2 was normalized by sesamin treatment. Finally, a selective Sirt3 inhibitor 3-TYP blocks all the protective role of sesamin, suggesting that a Sirt3-dependent effect of sesamin on cardiac remodeling. Conclusion: Sesamin improves cardiac function and prevents the development of cardiac hypertrophy via Sirt3/ROS pathway. Our results suggest the protective effect of sesamin on cardiac remolding.

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Sesamol ameliorates diet‐induced obesity in C57BL/6J mice and suppresses adipogenesis in 3T3‐L1 cells via regulating mitochondria‐lipid metabolism

Abstract: Taken together, this study provides compelling evidence that sesamol supplementation reduced adipocyte size and adipogenesis of diet-induced obesity by regulating mitochondria lipid metabolism.

Cited by 60 publications

References 45 publications

Sesamol Induces Human Hepatocellular Carcinoma Cells Apoptosis by Impairing Mitochondrial Function and Suppressing Autophagy

Sesamol Induces Human Hepatocellular Carcinoma Cells Apoptosis by Impairing Mitochondrial Function and Suppressing Autophagy

(‐)‐Epigallocatechin‐3‐gallate Ameliorates Insulin Resistance and Mitochondrial Dysfunction in HepG2 Cells: Involvement of Bmal1

Sesamin Protects Against Cardiac Remodeling Via Sirt3/ROS Pathway

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