Sesamin ameliorates arterial dysfunction in spontaneously hypertensive rats via downregulation of NADPH oxidase subunits and upregulation of eNOS expression

Zhang, Junxiu; Jie-ren, Yang; Chen, Guoxiang; Tang, Li‐Juan; Li, Wen‐Xing; Yang, Hui; Kong, Xiang

doi:10.1038/aps.2013.1

Cited by 35 publications

(42 citation statements)

References 71 publications

(77 reference statements)

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“…47 In the present study, we demonstrate that the VSMC and aorta from SHRs exhibit decreased levels of NO which may be because of the decreased expression of eNOS as well as to the enhanced levels of O 2 − . In this regard, the decreased expression of eNOS and NO has been reported in cardiac myocyte and vascular smooth muscle of hypertensive animals 35,48,49 and suggests that the increased BP in SHRs may also be attributed to the decreased levels of NO. Furthermore, eNOS knockout mice were shown to exhibit increased BP.…”

Section: Discussionmentioning

confidence: 86%

“…Furthermore, our results showing that the levels of ONOO − and nitrotyrosine were increased in VSMC, aorta, and kidney from SHRs that may be formed by the reaction of NO with O 2 − are in accordance with the studies of other investigators who have also reported an increased levels of ONOO − and nitrotyrosine content in aorta and mesenteric arteries as well as in kidney from SHRs without and with typical symptoms of metabolic syndrome. 35,36,49 However, we report for the first time that in vivo C-ANP [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] treatment decreased the enhanced levels of ONOO − and nitrotyrosine in VSMC, aorta, and kidney from SHRs. Thus, it may be suggested that C-ANP 4-23 -induced decreased levels of NO because of decreased expression of eNOS, O 2 − , and ONOO− and resultant decreased nitroxidative stress may be responsible for C-ANP 4-23 -induced attenuation of high BP.…”

Section: Discussionmentioning

confidence: 99%

“…Because SHRs have been shown to exhibit decreased expression of eNOS, 35 it was of interest to examine whether C-ANP 4-23 -induced decreased BP in SHRs is also attributed to its ability to augment the expression of eNOS. To test this, we determined the effect of in vivo C-ANP 4-23 treatment on the levels of eNOS in VSMC, aorta, and kidney from SHRs and WKY rats.…”

Section: Effect Of In Vivo C-anp 4-23 Treatment On the Levels Of Enosmentioning

confidence: 99%

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Natriuretic Peptide Receptor-C Attenuates Hypertension in Spontaneously Hypertensive Rats

Sarkar

Brochu

et al. 2014

Hypertension

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Abstract-C-Atrial natriuretic peptide (ANP) 4-23 , a ring deleted analog of ANP that specifically interacts with natriuretic peptide receptor-C (NPR-C), has been shown to decrease the enhanced expression of Giα proteins implicated in the pathogenesis of hypertension. In the present study, we investigated whether in vivo treatment of spontaneously hypertensive rats (SHRs) with C-ANP 4-23 could attenuate the development of high blood pressure (BP) and explored the underlying mechanisms responsible for this response. Intraperitoneal injection of C-ANP 4-23 at the concentration of 2 or 10 nmol/kg body weight to prehypertensive SHRs attenuated the development of high BP, and at 8 weeks it was decreased by ≈20 and 50 mm Hg, respectively; however, this treatment did not affect BP in Wistar-Kyoto rats. C-ANP 4-23 treatment of adult SHRs for 2 weeks also attenuated high BP, heart rate, and restored the impaired vasorelaxation toward control levels. In addition, the enhanced levels of superoxide anion (O 2 − ), peroxynitrite, NADPH oxidase activity, and the enhanced expression of Giα proteins, NOX4, p47 phox , nitrotyrosine, and decreased levels of endothelial nitric oxide synthase (eNOS or NOS3) and NO in SHRs were attenuated by C-ANP 4-23 treatment; however, the altered levels of NPR-A/NPR-C were not affected by this treatment. In conclusion, these results indicate that NPR-C activation by C-ANP 4-23 attenuates the development of high BP in SHRs through the inhibition of enhanced levels of Giα proteins and nitroxidative stress and not through eNOS/ cGMP pathway and suggest that NPR-C ligand may have the potential to be used as therapeutic agent in the treatment of Li et al NPR-C and Blood Pressure Regulation 847injection of pertussis toxin in prehypertensive rats (2-week-old SHRs) has been reported to prevent the development of BP,22 suggesting the implication of enhanced expression of Gi proteins in the pathogenesis of hypertension. C-ANP 4-23 that specifically interacts with NPR-C has been reported to decrease the levels of Giα proteins in A10 vascular smooth muscle cell (VSMC) 23 and in VSMC from SHRs. 24 In addition, C-ANP 4-23 was also shown to attenuate the enhanced oxidative stress in VSMC from SHRs 24 that contributes to the enhanced expression of Giα proteins in SHRs.25 Taken together, it may be possible that C-ANP 4-23 treatment of SHRs could also attenuate the high BP in SHRs. The present study was undertaken to investigate the effect of in vivo administration of C-ANP 4-23 on the development of high BP in SHRs. We have provided the first evidence that the treatment of prehypertensive SHRs with C-ANP 4-23 , an activator of NPR-C, attenuates the development of high BP in SHRs through the inhibition of enhanced expression of Gi proteins and oxidative stress.We have provided the first evidence that NPR-C activation by C-ANP 4-23 attenuates high BP through decreasing the enhanced oxidative stress and the augmented levels of Giα proteins. From these results it may be suggested that C-ANP [4][5][6][7][8][9][10][...

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Effect Of In Vivo C-anp 4-23 Treatment On the Levels Of Enosmentioning

confidence: 99%

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Natriuretic Peptide Receptor-C Attenuates Hypertension in Spontaneously Hypertensive Rats

Sarkar

Brochu

et al. 2014

Hypertension

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“…The bands were detected using a chemiluminescence assay (ECL Plus, Beyotime Institute of Biotechnology) and evaluated by densitometry using Image-Pro Plus 6.0 software (Media Cybernetics Inc, Rockville, MD). The relative density values of each band normalized to -actin were presented [18].…”

Section: Reverse Transcription-polymerase Chain Reaction (Rt-pcr) Anamentioning

confidence: 99%

“…Primary antibodies against nNOS diluted in PBS containing 0.1% BSA and 0.01% Tween 20 were applied to slides for 12 hours at 4 C. After two washes with PBS containing 0.01% Tween 20, slides were processed using the ABC and DAB staining kit (Vector Laboratories, Burlingame, CA) and slides were dehydrated and permanently mounted. This study used PBS instead of nNOS antibody as a negative control [18].…”

Section: Nnos Immunohistochemistry Of Hippocampal Formationmentioning

confidence: 99%

Dexmedetomidine renders a brain protection on hippocampal formation through inhibition of nNOS-NO signalling in endotoxin-induced shock rats

2014

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Effects of sesame (Sesamum indicum L.) and bioactive compounds (sesamin and sesamolin) on inflammation and atherosclerosis: A review

Hadipour,

Emami,

Tayarani‐Najaran

et al. 2023

Food Science & Nutrition

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Inflammation, oxidative stress, obesity, infection, hyperlipidemia, hypertension, and diabetes are the main causes of atherosclerosis, which in the long term lead to hardening of the arteries. In the current study, we reviewed recent findings of the mechanism of sesame and its active compounds of sesamin and sesamolin regulates on atherosclerosis. Sesame can decrease the lipid peroxidation and affect the enzymes, which control the balance of oxidative status in the body. Besides modulating the inflammatory cytokines, sesame regulates the main mediators of the signaling pathways in the process of inflammation, such as prostaglandin E2 (PGE2), nuclear factor kappa light‐chain enhancer of activated B cells (NF‐kB) and peroxisome proliferator‐activated receptor gamma (PPAR‐γ). Sesame decreases the growth of different pathogens. It fights against obesity and helps to reduce weight, body mass index (BMI), waist circumference, and lipid count of serum and liver. In addition to lowering fasting blood sugar (FBS), it decreases the hemoglobin A1c (HbA1c) and glucose levels and improves insulin function. With high content of linoleic acid, α‐linolenic acid, and total polyunsaturated fatty acid (PUFA), sesame efficiently controls the blood plasma lipids and changes the lipid profile. In the case of hypertension, it maintains the health of endothelium through multiple mechanisms and conserves the response of the arteries to vasodilation. PUFA in sesame suppresses blood clotting and fibrinogen activity. All the mentioned properties combat atherosclerosis and hardening of blood vessels, which are detailed in the present review for sesame.

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Sesamin ameliorates arterial dysfunction in spontaneously hypertensive rats via downregulation of NADPH oxidase subunits and upregulation of eNOS expression

Abstract: Long-term treatment with sesamin improves arterial function in SHR through the upregulation of eNOS expression and downregulation of p22(phox) and p47(phox) expression.

Cited by 35 publications

References 71 publications

Natriuretic Peptide Receptor-C Attenuates Hypertension in Spontaneously Hypertensive Rats

Natriuretic Peptide Receptor-C Attenuates Hypertension in Spontaneously Hypertensive Rats

Dexmedetomidine renders a brain protection on hippocampal formation through inhibition of nNOS-NO signalling in endotoxin-induced shock rats

Effects of sesame (Sesamum indicum L.) and bioactive compounds (sesamin and sesamolin) on inflammation and atherosclerosis: A review

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