Serum Vitellogenin Levels and Reproductive Impairment of Male Japanese Medaka (Oryzias latipes) Exposed to 4-tert-Octylphenol

Gronen, Suzanne; Denslow, Nancy D.; Manning, Steve; Barnes, Sue; Barnes, David; Brouwer, Marius

doi:10.2307/3434542

Cited by 15 publications

(12 citation statements)

References 34 publications

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“…The VTG induction in fish exposed to estrogens has not yet been clearly correlated with reproductive impairment. Gronen et al [18] found that male serum VTG levels were significantly correlated with fertilization after adult male medaka were exposed to octylphenol (OP) for 21 d. However, Kramer et al [23] showed that VTG production in male fathead minnow treated with estradiol (E 2 ) for 19 d was not correlated with egg production. In the present study, although the amount of VTG induced in males exposed to ≥116 ng/L of EE 2 was higher than that induced in females in each treatment group, no reproductive impairment was observed, except in the 488 ng/L treatment group.…”

Section: Discussionmentioning

confidence: 99%

“…Medaka is also a suitable test organism for investigations of developmental abnormalities of gonads, because this species develops an intersex condition (testis‐ova) in the gonads even if exposed to estrogenic substances after the period of sex differentiation [16,17]. Moreover, VTG induction has already been observed in male medaka after treatment with environmental estrogens [18,19]. Therefore, toxicity testing with mature medaka offers an integrative approach for determining the relationship between reproductive impairment and gonadal intersexuality as well as for determining physiological alterations, such as VTG induction in males exposed to environmental estrogens.…”

Section: Introductionmentioning

confidence: 99%

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Effect of ethinylestradiol on the reproduction and induction of vitellogenin and testis‐ova in medaka (Oryzias latipes)

Seki

Yokota

Matsubara

et al. 2002

Enviro Toxic and Chemistry

144

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Mature medaka (Oryzias latipes) were exposed to ethinylestradiol (EE2) at measured concentrations of 32.6, 63.9, 116, 261, and 488 ng/L for 21 d under flow-through conditions. Effects on reproductive success of the fish as well as on gonadal condition and vitellogenin (VTG) induction were assessed. A significant decrease in fecundity was observed only at the highest EE2 concentration, whereas hepatic VTG concentration in males was increased at concentrations of 63.9 ng/L and greater. In addition, an intersex condition (testis-ova) of the gonad was observed in male medaka exposed to EE2 concentrations of > or = 63.9 ng/L. Overall, these results indicate that the lowest-observed-effect concentrations of EE2 based on reproduction versus induction of both VTG and testis-ova in the medaka were 488 and 63.9 ng/L, respectively. Thus, the physiological and histological measurements were approximately eightfold more responsive to the EE2 exposure than were overt reproductive effects. This suggests that the elevated VTG levels and testis-ova may not be definitely responsible for reproductive impairment of the fish.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of ethinylestradiol on the reproduction and induction of vitellogenin and testis‐ova in medaka (Oryzias latipes)

Seki

Yokota

Matsubara

et al. 2002

Enviro Toxic and Chemistry

144

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“…Our ¢ndings that testis size and normal spermatozoal elongation are una¡ected support this interpretation. Male Japanese medaka (O. latipes) show compromised fertility and elevated levels of vitellogenin after a similar short exposure period of only 21 days to the xenoestrogenic alkylphenol 4-tert-octylphenol (Gronen et al 1999) but it is not known whether sperm numbers were compromised.…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, lactational exposure of male rat pups to nonylphenols resulted in a lower epididymal sperm count at adulthood (Lee et al 1999). Japanese medaka males (Oryzias latipes) exposed to 20^230 ppb 4-tert-octylphenol showed reduced fertility and histological examinations revealed that spermatogenesis had been inhibited (Gronen et al 1999). Nickel sulphate directly generated declines in sperm counts of adult male mice (Pandey et al 1999) while an intraperitoneal dose (250 mg kg 71 body weight) actually resulted in an increase in epididymal sperm count but a depletion in seminiferous tubules (Contreras & BustosObregon 1999).…”

Section: Introductionmentioning

confidence: 99%

Reduced sperm counts in guppies ( Poecilia reticulata ) following exposure to low levels of tributyltin and bisphenol A

Haubruge

Petit

Gage

2000

Proc. R. Soc. Lond. B

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There is increasing evidence that normal male reproductive function can be disrupted by exposure to pollutants in the environment that can exogenously mimic, antagonize or block sex-hormone function. One possible consequence of exposure to these xenobiotics is disruption to spermatogenesis, but results thus far provide only indirect and inconsistent evidence. In this study we exposed adult male guppies (Poeciliidae: Teleostei) to environmentally relevant levels of the common xenobiotics tributyltin (11.2-22.3 ngl-1) and bisphenol A (274-549 micrograms l-1) in experimental aquaria. After 21 days of exposure, we found significant declines (by 40-75%) in total sperm counts for male fishes exposed to tributyltin and bisphenol A compared with controls. This short-term decline in sperm count is unlikely to be the result of endocrine-mediated alteration of the germ line, and we found no change in testis size or sperm lengths between treatments. However, Sertoli cells, which facilitate the transport of maturing sperm into the testicular deferent duct (where they are stored prior to ejaculation), are directly sensitive to xenobiotic action and it is therefore possible that spermatogenesis was inhibited through in vivo interference with normal Sertoli-cell function.

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“…In point of fact, there is no evidence that vitellogenin affects reproductive behavior in fish (Mills and Chichester, 2005). However, some researchers have obtained a correlation between vitellogenin induction and reproductive endpoints such as fecundity and fertilization (Gronen et al. , 1999; Tilton et al.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Estrogens, River Water, and Treated Wastewater on Vitellogenin Induction of Japanese Medaka¹

Kitamura

Miyajima

Higashitani

et al. 2009

J American Water Resour Assoc

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The objective of this study is to evaluate the effect of estrogens on fish endocrine disruption in river water and treated wastewater. Endogenous estrogen estrone (E1), 17b-estradiol (E2), river water, and treated wastewater were used for exposure tests on male Japanese medaka. Vitellogenin induction in male medaka was regarded as the endpoint of endocrine disruption. The effective concentrations of E1 and E2 on vitellogenin induction in medaka were evaluated by breeding medaka for 14 days in tanks with various concentrations of E1 or E2. Vitellogenin induction increased with elapse of time during exposure, with higher estrogen concentrations causing greater vitellogenin induction. According to the test results, the lowest observed effect concentrations (LOECs) of E1 and E2 were estimated to be 31.6 and 5.0 ng ⁄ l, respectively; and the E2 equivalent (EEQ) LOEC value of E1 was 5.2 ng-E2 ⁄ l, derived by multiplying the relative potency of E1 by that of E2 as estimated in this study. In continuous exposure tests using river water or treated wastewater, the estrogenic activity, an index of total estrogenic potential measured by yeast screen assay and expressed as EEQ, varied widely during the exposure tests, and significant vitellogenin induction was observed after several days of high levels of estrogenic activity in water. Vitellogenin concentration tended to significantly increase if EEQ exceeded the level of 5 ng-E2 ⁄ l. The threshold value was substantially the same as the results for E1 and E2 exposure tests of medaka. Consequently, EEQ over 5 ng-E2 ⁄ l was revealed to have the potential to cause endocrine disruption of male medaka. As estrogenic activity exceeding 5 ng-E2 ⁄ l was observed in some rivers in Japan, total estrogenic potential needs to be further decreased at wastewater treatment plants to prevent fish endocrine disruption.

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Serum Vitellogenin Levels and Reproductive Impairment of Male Japanese Medaka (Oryzias latipes) Exposed to 4-tert-Octylphenol

Abstract: We thank Jeffrey Lotz for help with statistical analysis and Nancy Brown-Peterson and Rena Krol for histological advice. This study was supported in part by the Mississippi-Alabama Sea Grant Consortium grant award NA86RG0039. S.G. was supported by a USM master's research assistantship.

Cited by 15 publications

References 34 publications

Effect of ethinylestradiol on the reproduction and induction of vitellogenin and testis‐ova in medaka (Oryzias latipes)

Effect of ethinylestradiol on the reproduction and induction of vitellogenin and testis‐ova in medaka (Oryzias latipes)

Reduced sperm counts in guppies ( Poecilia reticulata ) following exposure to low levels of tributyltin and bisphenol A

The Effect of Estrogens, River Water, and Treated Wastewater on Vitellogenin Induction of Japanese Medaka¹

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Serum Vitellogenin Levels and Reproductive Impairment of Male Japanese Medaka (Oryzias latipes) Exposed to 4-tert-Octylphenol

Abstract: We thank Jeffrey Lotz for help with statistical analysis and Nancy Brown-Peterson and Rena Krol for histological advice. This study was supported in part by the Mississippi-Alabama Sea Grant Consortium grant award NA86RG0039. S.G. was supported by a USM master's research assistantship.

Cited by 15 publications

References 34 publications

Effect of ethinylestradiol on the reproduction and induction of vitellogenin and testis‐ova in medaka (Oryzias latipes)

Effect of ethinylestradiol on the reproduction and induction of vitellogenin and testis‐ova in medaka (Oryzias latipes)

Reduced sperm counts in guppies ( Poecilia reticulata ) following exposure to low levels of tributyltin and bisphenol A

The Effect of Estrogens, River Water, and Treated Wastewater on Vitellogenin Induction of Japanese Medaka1

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The Effect of Estrogens, River Water, and Treated Wastewater on Vitellogenin Induction of Japanese Medaka¹