Serum vitamin D and colonic vitamin D receptor in inflammatory bowel disease

Abreu-Delgado, Yamilka; Isidro, Raymond A; Torres, Esther A.; Gonzalez, Alexandra; Cruz, Myrella L.; Isidro, Angel A.; González-Keelan, Carmen; Medero, Priscilla; Appleyard, Caroline B.

doi:10.3748/wjg.v22.i13.3581

Cited by 35 publications

(39 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In contrast to findings in humans with IBD, which have significantly decreased intestinal VDR expression, 39,52 we found no difference in VDR expression in the duodenum of dogs with gastrointestinal inflammation. Assessments of inflammation by several criteria and by multiple methods showed no significant decline in VDR (Fig 4).…”

Section: Discussioncontrasting

confidence: 99%

“…The second major finding of this study was that duodenal VDR expression, both RNA and protein, did not decrease with inflammation. This contrasts with the changes reported in both human and rodent colonic inflammation …”

Section: Discussioncontrasting

confidence: 95%

“…This contrasts with the changes reported in both human and rodent colonic inflammation. 39,40 Our study is the first to establish that canine skin expresses high levels of VDR, a finding that is similar to those observed in other species. 41 This finding is noteworthy, given the fact that the dog does not produce significant levels of cutaneous vitamin D from ultraviolet light.…”

Section: Discussionsupporting

confidence: 86%

“…There was only weak VDR expression in the colon in healthy dogs, a finding which is different to people and other species. 39,51 In human ulcerative colitis and in mouse models of colitis, VDR expression is negatively correlated with colonic inflammation.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Vitamin D Receptor Expression in Dogs

Cartwright

Gow

Milne

et al. 2018

Veterinary Internal Medicne

View full text Add to dashboard Cite

BackgroundThere is growing evidence linking low blood vitamin D concentration to numerous diseases in people and in dogs. Vitamin D influences cellular function by signaling through the vitamin D receptor (VDR). Little is known about which non‐skeletal tissues express the VDR or how inflammation influences its expression in the dog.ObjectivesTo define which non‐skeletal canine tissues express the VDR and to investigate expression in inflamed small intestine.AnimalsThirteen non‐skeletal tissues were collected prospectively from 6 control dogs. Thirty‐five dogs diagnosed with a chronic enteropathy (CE) and 24 control dogs were prospectively enrolled and duodenal biopsies were evaluated for VDR expression.MethodsProspective; blinded assessment of canine intestinal VDR. Dogs with CE were included once other identifiable causes of intestinal disease were excluded. Age matched controls were included with no intestinal clinical signs. VDR expression was assessed immunohistochemically in all samples, using a Rat IgG VDR monoclonal antibody. Quantitative real‐time polymerase chain reaction (qPCR) was also used for duodenal biopsies.ResultsVDR expression as assessed by immunohistochemistry (IHC) was highest in the kidney, duodenum, skin, ileum and spleen, and weak in the colon, heart, lymph node, liver, lung, and ovary. Gastric and testicular tissue did not express the VDR. There was no statistical difference in duodenal VDR expression between the 24 healthy dogs and 34 dogs with CE when quantified by either qPCR (P = 0.87) or IHC (P = 0.099).Conclusions and Clinical ImportanceThe lack of down regulation of VDR expression in inflamed intestine contrasts with previous studies in humans. Our findings support future studies to investigate whether vitamin D and its analogues can be used to modulate intestinal inflammation in the dog.

show abstract

Section: Discussioncontrasting

confidence: 99%

Section: Discussioncontrasting

confidence: 95%

Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Vitamin D Receptor Expression in Dogs

Cartwright

Gow

Milne

et al. 2018

Veterinary Internal Medicne

View full text Add to dashboard Cite

show abstract

“…Furthermore, Abreu-Delgado et al[65] reported that levels of serum vitamin D correlate positively with colonic VDR expression in visually normal mucosa; whereas inflammation correlates negatively with colonic VDR expression in visually diseased mucosa. The VDR needs further research.…”

Section: Ibd- and Autophagy-related Genetic Variantsmentioning

confidence: 99%

Role of autophagy in the pathogenesis of inflammatory bowel disease

Iida

Onodera

Nakase

2017

WJG

111

View full text Add to dashboard Cite

Inflammatory bowel disease (IBD) results from a complex series of interactions between susceptibility genes, the environment, and the immune system. Recently, some studies provided strong evidence that the process of autophagy affects several aspects of mucosal immune responses. Autophagy is a cellular stress response that plays key roles in physiological processes, such as innate and adaptive immunity, adaptation to starvation, degradation of aberrant proteins or organelles, antimicrobial defense, and protein secretion. Dysfunctional autophagy is recognized as a contributing factor in many chronic inflammatory diseases, including IBD. Autophagy plays multiple roles in IBD pathogenesis by altering processes that include intracellular bacterial killing, antimicrobial peptide secretion by Paneth cells, goblet cell function, proinflammatory cytokine production by macrophages, antigen presentation by dendritic cells, and the endoplasmic reticulum stress response in enterocytes. Recent studies have identified susceptibility genes involved in autophagy, such as NOD2, ATG16L1, and IRGM, and active research is ongoing all over the world. The aim of this review is a systematic appraisal of the current literature to provide a better understanding of the role of autophagy in the pathogenesis of IBD. Understanding these mechanisms will bring about new strategies for the treatment and prevention of IBD.

show abstract