Serum thymidine kinase activity in clinically healthy and diseased horses: A potential marker for lymphoma

Larsdotter, Sara; Nostell, Katarina; Euler, Henrik von

doi:10.1016/j.tvjl.2015.01.019

Cited by 26 publications

(32 citation statements)

References 19 publications

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“…Anemia and thrombocytopenia often are reported but were fairly uncommon in our case series. Thymidine kinase recently was reported to be a useful indicator of lymphoma in horses . Only 1 horse in our study had this analyte measured, and it was found to be within normal limits.…”

Section: Discussionmentioning

confidence: 49%

See 1 more Smart Citation

Retrospective evaluation of clinical outcome after chemotherapy for lymphoma in 15 equids (1991‐2017)

Luethy

Frimberger²,

Bedenice

et al. 2019

Veterinary Internal Medicne

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Background Prognosis associated with lymphoma in horses is poorly characterized, and treatment is often palliative. Long‐term outcome after chemotherapy for horses with lymphoma is not well documented. Objective To report long‐term outcome of horses with lymphoma treated with chemotherapy. Animals Fifteen equids. Methods Retrospective case series. Medical record search and call for cases on the ACVIM listserv for horses treated with chemotherapy for lymphoma. Results Fifteen cases with adequate data were identified. Complete remission was achieved in 5 horses (33.3%), partial response was achieved in 9 equids (60%), and stable disease was achieved in 1 horse. Overall response rate was 93.3% (14/15). Overall median survival time was 8 months (range, 1‐46 months). Nine horses experienced a total of 14 adverse effects attributable to chemotherapy. Adverse effects were graded according to the Veterinary Cooperative Oncology Group common terminology criteria for adverse events grading system (grade 1 alopecia, n = 2; grade 1 neutropenia, n = 2; grade 1 lymphopenia, n = 3; grade 1 lethargy, n = 1; grade 2 neurotoxicity, n = 1; grade 2 colic, n = 1; grade 1 hypersensitivity, n = 1; grade 2 hypersensitivity, n = 2; grade 5 hypersensitivity, n = 1). Higher grade adverse effects most commonly were associated with doxorubicin administration (n = 4), including 1 horse that died 18 hours post‐administration. Conclusions and Clinical Importance Chemotherapy can be used successfully for treatment of horses with lymphoma. Adverse effects, most commonly mild, occurred in approximately two‐thirds of treated horses.

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Section: Discussionmentioning

confidence: 49%

“…Results were available for 14 of the 15 equids included in the study. Thymidine kinase activity was evaluated in 1 horse and was normal at 1.5 U/L (reference range, <2.7 U/L) . Three of 4 horses tested were equine herpes virus‐5 (EHV‐5) PCR positive on biopsy specimens of neoplastic tissue (2 cutaneous TCRLBCL, 1 multicentric TCRLBCL).…”

Section: Resultsmentioning

confidence: 99%

Retrospective evaluation of clinical outcome after chemotherapy for lymphoma in 15 equids (1991‐2017)

Luethy

Frimberger²,

Bedenice

et al. 2019

Veterinary Internal Medicne

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“…Thymidine kinase (TK) is a cellular enzyme involved in DNA synthesis and therefore increases when cells are in replicative stages. A study in horses showed that increases in TK type 1 is indicative of hematopoietic neoplasia and could be used as a tumour biomarker (Larsdotter et al 2015). However, it has a low sensitivity (74 %) and specificity (14 %) at the suggested cut-off value of 2.2 U/L (Larsdotter et al 2015) and therefore this test is not suitable as a single marker for lymphoma.…”

Section: Discussionmentioning

confidence: 99%

Advanced multicentric lymphoma in a Belgian Draft Horse mare

Siegers¹,

Oosterbaan²,

Wollenberg³

et al. 2017

PHK

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Summary: A 15-year old draft horse mare was presented to the University Clinic for evaluation of lethargy, anorexia, ptyalism, weight loss and ventral oedema. Clinical examination and rectal palpation revealed generalised lymphadenopathy and numerous firm subcutaneous and abdominal masses of various sizes. Transcutaneous ultrasonography revealed significant bilateral pleural fluid accumulation and a single hypoechoic structure in the abdomen lateral to the liver. Blood analysis showed several abnormalities including a marked leucocytosis with an increased number of segmented neutrophils, atypical lymphocytes and monocytosis, suggestive of leukaemic lymphoma. A significant hyperproteinaemia with a hypoalbuminemia and a monoclonal gammopathy was identified. At necropsy myriad masses presented through the whole body. Histology confirmed the suspicion of lymphoma, which was classified as a T-cell rich B-cell multicentric lymphoma. This article describes the clinical and pathologic findings of this case of leukaemic lymphoma.

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“…However, in animals the use of kinase inhibitor treatment has been limited. Select tyrosine kinase inhibitors have been approved for the treatment of cancer in companion animals (13,14) as well as preliminary investigations of similar applications in horses (15). A category of kinase inhibitors, referred to as bumped kinase inhibitors (BKIs), target calcium-dependent protein kinases belonging to parasites of human and veterinary importance, including Toxoplasma gondii, Plasmodium falciparum, and Cryptosporidium parvum (16).…”

Section: Introductionmentioning

confidence: 99%

From Beef to Bees: High-Throughput Kinome Analysis to Understand Host Responses of Livestock Species to Infectious Diseases and Industry-Associated Stress

et al. 2020

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Within human health research, the remarkable utility of kinase inhibitors as therapeutics has motivated efforts to understand biology at the level of global cellular kinase activity (the kinome). In contrast, the diminished potential for using kinase inhibitors in food animals has dampened efforts to translate this research approach to livestock species. This, in our opinion, was a lost opportunity for livestock researchers given the unique potential of kinome analysis to offer insight into complex biology. To remedy this situation, our lab developed user-friendly, cost-effective approaches for kinome analysis that can be readily incorporated into most research programs but with a specific priority to enable the technology to livestock researchers. These contributions include the development of custom software programs for the creation of species-specific kinome arrays as well as comprehensive deconvolution and analysis of kinome array data. Presented in this review are examples of the application of kinome analysis to highlight the utility of the technology to further our understanding of two key complex biological events of priority to the livestock industry: host immune responses to infectious diseases and animal stress responses. These advances and examples of application aim to provide both mechanisms and motivation for researchers, particularly livestock researchers, to incorporate kinome analysis into their research programs.

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Serum thymidine kinase activity in clinically healthy and diseased horses: A potential marker for lymphoma

Cited by 26 publications

References 19 publications

Retrospective evaluation of clinical outcome after chemotherapy for lymphoma in 15 equids (1991‐2017)

Retrospective evaluation of clinical outcome after chemotherapy for lymphoma in 15 equids (1991‐2017)

Advanced multicentric lymphoma in a Belgian Draft Horse mare

From Beef to Bees: High-Throughput Kinome Analysis to Understand Host Responses of Livestock Species to Infectious Diseases and Industry-Associated Stress

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