Serum thromboxane in the horse and its inhibition by aspirin, phenylbutazone and flunixin

Lees, P.; Ewins, C.P.; Taylor, J. B.; Sedgwick, A. D.

doi:10.1016/0007-1935(87)90024-8

Cited by 78 publications

(65 citation statements)

References 25 publications

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“…Based on these fi ndings, leukocytes (500 × 10 6 cells) and/or platelets (5 × 10 6 cells) were incubated for 22 h with LPS (10 pg to 1 µg/ml), with or without aspirin (100 µM) or the PAF antagonist, WEB 2086 (100 nM or 1 µM) [30]. TxA 2 concentrations were determined by radioimmunoassay, as previously described [31].…”

Section: Effect Of Lps On Txa 2 Productionmentioning

confidence: 99%

Endotoxin-induced activation of equine platelets: evidence for direct activation of p38 MAPK pathways and vasoactive mediator production

et al. 2007

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Section: Effect Of Lps On Txa 2 Productionmentioning

confidence: 99%

Endotoxin-induced activation of equine platelets: evidence for direct activation of p38 MAPK pathways and vasoactive mediator production

et al. 2007

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“…Samples were kept at -20°C prior to analysis of the eicosanoids, which was carried out by RIA. The 12-HETE RIA was performed using a commercially available kit (Metachem Diagnostics, UK) and the RIA for TxB 2 by a previously described method employing a commercially available antibody to TxB 2 [19]. The amounts of TxB 2 or 12-HETE have been expressed as ng per 10 8 platelets.…”

Section: Production Of Eicosanoids By Platelets From Normal and Sweetmentioning

confidence: 99%

Differential activation of platelets from normal and allergic ponies by PAF and ADP

Bailey

Andrews

Elliott

et al. 2000

Inflammation Research

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Objective and Design: Altered platelet responsiveness has been demonstrated in human atopic dermatitis. This study has compared the in vitro function of platelets from normal ponies and those with the allergic skin disease, sweet itch. Subjects: Ponies with a clinical history of sweet itch and normal ponies were used as blood donors. Methods: PAF and ADP-induced platelet aggregation was measured and TxB 2 production quantitated at the time of maximal aggregation; 12-HETE was additionally measured in some samples. Agonist-induced release of 3 [H]5-HT was also studied. Results: Although both PAF and ADP caused equine platelet aggregation, only PAF stimulated eicosanoid and 5-HT release. There were no differences between the responses of platelets from allergic and normal ponies to PAF or ADP (analysis of variance). Conclusions: There is no evidence of altered platelet responsiveness in ponies with sweet itch. The profile of responses to PAF and ADP suggest differential activation of intracellular signalling pathways in equine platelets.

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“…In horses, ASA is not routinely used for the treatment of inflammatory conditions because of its short half-life, low potency and resulting high dosages needed [1-3]. Still, ASA and SA pharmacokinetics are of interest because SA is the only NSAID with threshold levels for plasma and urine in equine sports [4-6].…”

Section: Introductionmentioning

confidence: 99%

“…However, threshold levels might increase the risk of ASA being abused for therapeutic purposes up to the threshold in competition horses [8]. In this context, we were interested in synovial fluid concentrations after systemic administration because none of the numerous studies that have dealt with salicylate pharmacokinetics in humans [9-11], cattle [12], and horses [1,3] has so far explored the distribution of ASA or SA into joint fluid. Therefore, the study presented aimed to investigate to what extent ASA is distributed into the synovial fluid of horses after systemic administration.…”

Section: Introductionmentioning

confidence: 99%

Synovial distribution of “systemically” administered acetylsalicylic acid in the isolated perfused equine distal limb

et al. 2013

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BackgroundThis study investigated synovial concentrations of acetylsalicylic acid (ASA) and its metabolite salicylic acid (SA) in the equine fetlock joint following systemic administration of ASA. Salicylates were chosen because SA is the only nonsteroidal anti-inflammatory drug for which threshold levels exist for plasma and urine in equine sports. To avoid animal experiments, the study was conducted using an ex vivo model of the isolated perfused equine distal limb in combination with plasma concentrations obtained from literature.Salicylate concentrations in the joint were determined using microdialysis and high performance liquid chromatography (HPLC). Any anti-inflammatory effect of synovial ASA concentrations was assessed using an ASA EC50 (half maximal effective concentration) determined in equine whole blood.ResultsThe ASA concentration in the synovial fluid (n = 6) reached a maximum of 4 μg/mL, the mean concentration over the entire perfusion period was 2 μg/mL. Maximum SA concentration was 17 μg/mL, the average was 14 μg/mL. ASA and SA concentration in the synovial fluid exceeded systemic concentrations 2 h and 3.5 h after “systemic” administration, respectively.ConclusionsASA and SA accumulated in the in the synovial fluid of the ex vivo model despite decreasing systemic concentrations. This suggests a prolonged anti-inflammatory effect within the joint that remains to be further elucidated.

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Serum thromboxane in the horse and its inhibition by aspirin, phenylbutazone and flunixin

Cited by 78 publications

References 25 publications

Endotoxin-induced activation of equine platelets: evidence for direct activation of p38 MAPK pathways and vasoactive mediator production

Endotoxin-induced activation of equine platelets: evidence for direct activation of p38 MAPK pathways and vasoactive mediator production

Differential activation of platelets from normal and allergic ponies by PAF and ADP

Synovial distribution of “systemically” administered acetylsalicylic acid in the isolated perfused equine distal limb

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