Serum testosterone levels measured by isotope dilution-liquid chromatography–tandem mass spectrometry in postmenopausal women versus those in women who underwent bilateral oophorectomy

Bui, Hong N.; Struys, Eduard A.; Martens, Frans; Ronde, Willem de; Thienpont, Linda M.; Kenemans, P.; Verhoeven, Marieke O.; Jakobs, Cornelis; Dijstelbloem, Hilde M.; Blankenstein, Marinus A.

doi:10.1258/acb.2010.009171

Cited by 47 publications

(25 citation statements)

References 15 publications

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“…Several studies also showed that women with bilateral oophorectomy had lower testosterone levels than those in natural postmenopausal women (92)(93)(94). Bui et al reported that a significant decrease was found in testosterone levels after bilateral oophorectomy, whereas no significant difference was found after natural menopause by using the developed isotope dilution-liquid chromatography-tandem mass spectrometry (95). However, more recently, it has been shown that levels of testosterone and DHEA-S did not differ significantly between surgically and naturally menopausal women with a mean age of 52.4 years (96).…”

Section: Site Of Androgen Production In Postmenopausal Womenmentioning

confidence: 99%

Androgen in postmenopausal women

et al. 2012

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Dynamic changes in circulating estradiol level, including increase at menarche and decrease at menopause, occur in a woman's lifetime. Circulating estradiol levels decrease drastically during the menopausal transition, though the levels differ among races. It has been reported that estradiol levels in both Japanese and Chinese women were lower than those in Caucasians, Hispanic and AfricanAmericans (1).Based on data obtained in a previous study (2), we re-analyzed data for 169 healthy men and 252 healthy women aged from 40 to 85 years in the population-based study with addition of data for premenopausal women. Change in circulating estradiol in women from the menopausal transition to postmenopause is characterized as follows : high estradiol level in premenopausal women is drastically decreased and estradiol level in postmenopausal women is significantly lower than that in agematched men. Mean estradiol levels were shown to be 20.1 pg/ml in men and 6.1 pg/ml in postmenopausal women (Figure 1). This dynamic decrease in REVIEW Androgen in postmenopausal women

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Section: Site Of Androgen Production In Postmenopausal Womenmentioning

confidence: 99%

Androgen in postmenopausal women

et al. 2012

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“…In this case, the synthesis of D 5 -GP and D 4 -DMBA could be used to provide an easily prepared isotope-labeled steroid hormone. Based on the previously described investigation, in this study D 5 -GP and D 4 -DMBA reagents were synthesized. D 5 -GP reagent (9 g) was obtained with a yield of 89%.…”

Section: -37mentioning

confidence: 99%

A sensitive approach for simultaneous quantification of carbonyl and hydroxyl steroids using 96-well SPE plates based on stable isotope coded-derivatization-UPLC-MRM: method development and application

et al. 2018

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Steroid hormones are crucial substances that mediate a wide range of vital physiological functions. Because of the important biological significance of steroids, this paper presents a new targeted metabolic method based on adding stable isotope tags to hydroxyl containing and carbonyl containing steroid hormones with two pairs of synthesized derivatization reagents: deuterium 4-(dimethylamino)-benzoic acid (D 4 -DMBA), and D 5 -Girard P (D 5 -GP) using of ultra performance liquid chromatography-multiple reaction monitoring (UPLC-MRM). Firstly, an Oasis PRiME hydrophilic-lipophilic balance (HLB) 96-well solid phase extraction plate was used to pretreat a number of biological samples simultaneously. Secondly, hydroxyl and carbonyl steroids were labeled using two pairs of synthetic reagents, namely DMBA and D 4 -DMBA, and GP and D 5 -GP, respectively. Thirdly, the mixed products were detected using UPLC-MRM and the mass spectroscopy conditions were optimized. Methodology development showed that the sensitivity was enhanced 1 to >500-fold. Finally, the new method was applied to analysis of urine samples of healthy males, females and rats. The results revealed that the method can be sensitive and reliable for simultaneous quantification of steroid hormones containing hydroxyl and carbonyl groups in 12 min in a single run. This method provided a powerful tool for studying the metabolic mechanism of steroids and contributed to the development of targeted metabolomics.

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“…3 The internal standards were tested for purity prior to the development of the method and both internal standards co-eluted with endogenous testosterone. Even though our observations differ from those reported by Owen and Keevil, we agree that the choice of internal standard is crucial.…”

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Isotopically labelled testosterone derivatives as internal standards in liquid chromatography-tandem mass spectrometry

2013

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Isotopically labelled testosterone derivatives as internal standards in liquid chromatography-tandem mass spectrometryWe read with interest the recent paper by Owen and Keevil 1 regarding the importance of internal standard choice for testosterone measurement by liquidchromatography tandem-mass spectrometry (LC-MS/MS). The authors investigated the performance of three isotopically labelled internal standards for testosterone, i.e. D 2 -testosterone, 13 C 3 -testostosterone and D 5 -testosterone, using the same protocol and a substantial number of samples covering the whole clinical range. While we agree that a proper internal standard is imperative for accurate LC-MS/MS measurements, the observed differences illustrated by their data might have clinical impact and left a few questions.Stable isotope-dilution with 13 C-isotopes to correct for losses during sample preparation is considered to be the standard because 13 C-labelled compounds co-elute exactly with their endogenous counterpart. Moreover, the stability of deuterium labelled-isotopes is highly dependent on the location of the labels which might exchange with hydrogen-atoms. Nevertheless, it is generally accepted that any stable isotope could be sufficient. Comparison between

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Serum testosterone levels measured by isotope dilution-liquid chromatography–tandem mass spectrometry in postmenopausal women versus those in women who underwent bilateral oophorectomy

Cited by 47 publications

References 15 publications

Androgen in postmenopausal women

Androgen in postmenopausal women

A sensitive approach for simultaneous quantification of carbonyl and hydroxyl steroids using 96-well SPE plates based on stable isotope coded-derivatization-UPLC-MRM: method development and application

Isotopically labelled testosterone derivatives as internal standards in liquid chromatography-tandem mass spectrometry

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