Serum tau protein as a marker for the diagnosis of Creutzfeldt-Jakob disease

Noguchi‐Shinohara, Moeko; Hamaguchi, Tetsuya; Nozaki, Ichiro; Sakai, Kenji; Yamada, Masahito

doi:10.1007/s00415-011-5960-x

Cited by 39 publications

(33 citation statements)

References 23 publications

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“…This was also observed in Prnp 0/0 mice (FVB background) with increased tau phosphorylation at earlier stages [72]. Similar data have described tyr 18 phosphorylation of tau after ADDL treatment [41]. Our data confirm but also expand these observations to all the phospho-epitopes studied.…”

Section: Discussionsupporting

confidence: 81%

“…In fact, aggregated peptides in fibrillar forms failed to activate tau phosphorylation (e.g., tyr 18 residue) in contrast to the antibody-mediated PrP C aggregation in the membrane [37]. Thus PrP C reduces the effects of ADDLs in tau level modulation as well as their phosphorylation.…”

Section: Discussionmentioning

confidence: 96%

“…Creutzfeldt-Jakob's disease (CJD) patients [17,18]. In parallel, Wang and coworkers have described several changes in expression and phosphorylation levels of tau in infected hamsters.…”

Section: Introductionmentioning

confidence: 88%

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Role of PrPC Expression in Tau Protein Levels and Phosphorylation in Alzheimer’s Disease Evolution

et al. 2014

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Alzheimer's disease (AD) is characterized by the presence of amyloid plaques mainly consisting of hydrophobic -amyloid peptide (A) aggregates and neurofibrillary tangles (NFTs) composed principally of hyperphosphorylated tau. A oligomers have been described as the earliest effectors to negatively affect synaptic structure and plasticity in the affected brains, and cellular prion protein (PrP C ) has been proposed as receptor for these oligomers.The most widely accepted theory holds that the toxic effects of A are upstream of change in tau, a neuronal microtubule-associated protein that promotes the polymerization and stabilization of microtubules. However, tau is considered decisive for the progression of neurodegeneration, and indeed tau pathology correlates well with clinical symptoms such as dementia. Different pathways can lead to abnormal phosphorylation, and, as a consequence, tau aggregates into Paired Helical Filaments (PHF) and later on into NFTs.Reported data suggest a regulatory tendency of PrP C expression in the development of AD, and a putative relationship between PrP C and tau processing is emerging. However the role of tau/PrP C interaction in AD is poorly understood.In this study we show increased susceptibility to A derived diffusible ligands (ADDLs) in neuronal primary cultures from PrP C knock-out mice, compared to wild-type, which correlates with increased tau expression. Moreover, we found increased PrP C expression that paralleled with tau at early ages in an AD murine model, and in early Braak stages of AD in affected individuals. Taken together, these results suggest a protective role for PrP C in AD by down-regulating tau expression, and they point to this protein as being crucial in the molecular events that lead to neurodegeneration in AD.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 96%

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Role of PrPC Expression in Tau Protein Levels and Phosphorylation in Alzheimer’s Disease Evolution

et al. 2014

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“…Conflicting results exist regarding this issue, with some studies failing to find any influence of age and/or disease duration on t-Tau sensitivity [12,14,22], but other doing so [13,19]. Another small study showed that t-Tau could also be a useful marker for sCJD in serum samples [48]. In a recent report, it was observed that in CJD individuals, t-Tau levels and t-Tau to p-Tau ratios increased over time, and the combination of increased t-Tau levels and increased t-Tau to p-Tau ratios in CJD patients has a very high specificity against important differential diagnoses [49].…”

Section: Discussionmentioning

confidence: 99%

CSF Tau proteins reduce misdiagnosis of sporadic Creutzfeldt–Jakob disease suspected cases with inconclusive 14-3-3 result

et al. 2016

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Cerebrospinal fluid (CSF) 14-3-3 protein supports sporadic Creutzfeldt-Jakob (sCJD) diagnosis, but often leads to weak-positive results and lacks standardization. In this study, we explored the added diagnostic value of Total Tau (t-Tau) and phosphorylated Tau (p-Tau) in sCJD diagnosis, particularly in the cases with inconclusive 14-3-3 result. 95 definite sCJD and 287 patients without prion disease (non-CJD) were included in this study. CSF samples were collected in routine clinical diagnosis and analysed for 14-3-3 detection by Western blot (WB). CSF t-Tau and p-Tau were quantified by commercial ELISA kits and PRNP and APOE genotyping assessed by PCR-RFLP. In a regression analysis of the whole cohort, 14-3-3 protein revealed an overall accuracy of 82 % (sensitivity = 96.7 %; specificity = 75.6 %) for sCJD. Regarding 14-3-3 clear positive results, we observed no added value either of t-Tau alone or p-Tau/t-Tau ratio in the model. On the other hand, considering 14-3-3 weakpositive cases, t-Tau protein increased the overall accuracy of 14-3-3 alone from 91 to 94 % and specificity from 74 to 93 % (p \ 0.05), with no sensitivity improvement. However, inclusion of p-Tau/t-Tau ratio did not significantly improve the first model (p = 0.0595). Globally, t-Tau protein allowed a further discrimination of 65 % within 14-3-3 inconclusive results. Furthermore, PRNP MV genotype showed a trend to decrease 14-3-3 sensitivity (p = 0.051), but such effect was not seen on t-Tau protein.In light of these results, we suggest that t-Tau protein assay is of significant importance as a second marker in identifying 14-3-3 false-positive results among sCJD probable cases.

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“…Serum tau does not appear to accurately reflect the level of CSF tau as shown in multiple studies (33,34). Transient increases in plasma tau levels may appear as a result of ischemic stroke, hypoxic or traumatic brain injury (35,36) and thus interfere with an accurate measurement in AD pathology.…”

Section: Tau Proteins In Plasmamentioning

confidence: 99%

Blood-based biomarkers in Alzheimer’s disease: an overview on proteomic and lipidomic approaches

Ilinoiu

Tirintica

Lungu

et al. 2016

Revista Romana De Medicina De Laborator

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Serum tau protein as a marker for the diagnosis of Creutzfeldt-Jakob disease

Cited by 39 publications

References 23 publications

Role of PrPC Expression in Tau Protein Levels and Phosphorylation in Alzheimer’s Disease Evolution

Role of PrPC Expression in Tau Protein Levels and Phosphorylation in Alzheimer’s Disease Evolution

CSF Tau proteins reduce misdiagnosis of sporadic Creutzfeldt–Jakob disease suspected cases with inconclusive 14-3-3 result

Blood-based biomarkers in Alzheimer’s disease: an overview on proteomic and lipidomic approaches

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