Serum tartrate-resistant acid phosphatase (TRAP) as a biochemical marker of bone remodeling

Total and regional bone mass and biochemical markers of bone remodeling in metastatic prostate cancer

“…Therefore, all the TRAP measured in this study was osteoclast-dependent. Although other biological markers of bone resorption are currently in fashion [3,22], TRAP meets more than enough criteria for considering it an adequate marker of bone resorption: 1) It correlates significantly and inversely with estrogen levels before and after Gn-RH agonist treatment [23], and in normal circumstances TRAP decreases with menarche and increases after menopause [24], 2) TRAP is elevated in diseases that are accompanied by a high remodeling rate [25], 3) TRAP correlates negatively with bone mass [23] and positively with alkaline phosphatase [26], as confirmed by this and other studies [23,26], 4) TRAP is sensitive to the effect of drugs that slow remodeling and reduce osteoclastic activity [27], 5) Osteoclastic TRAP is similar to serum TRAP 5b, as shown by electrophoretic mobility on acid gel [28], 6) Its specificity, because circulating TRAP 5b is exclusively osteoclastic in origin, increases its value as a marker of bone remodeling, 7) Finally, its determination in blood is easy [7] and free from the potential errors of markers quantitated in urine. Therefore, we do not think that more expensive markers, which require more complicated and sophisticated techniques, are necessary for evaluating bone resorption.…”

“…Biochemical studies were performed in serum, using the BM/Hitachi Automated Analyzer System 717 (Boehringer Laboratories, Mannheim, Germany). TRAP was quantitated in serum in the Hitachi automated analyzer as the substrate (␣-naphthyl phosphate), using a reagent from Boehringer that reacts specifically with isoenzyme 5b synthesized by the osteoclast [7]. Twenty-four-hour urinary calcium excretion was determined by atomic absorption spec- troscopy, using a Perkin Elmer (Norfolk, CT) Model 5000 spectrophotometer.…”

“…Quantification of the 5b fraction of TRAP is more specific and useful as a marker of bone resorption [7] because the 5b fraction is synthesized only by osteoclasts and the cells of tricholeukemia [8]. However, hairy cells, in contrast with osteoclasts, do not release TRAP 5b into the blood stream [9,10], and so all circulating TRAP 5b is the product of osteoclastic synthesis [7].…”

Avaliação da influência da osteoporose induzida em ratas, e seu tratamento com alendronato e estrógeno, sobre o tecido ósseo ao redor de implantes

“…The systemic bone density was decreased relative to the SHAM group, as well as serum parameters of bone remodeling were altered. These results are in agreement with Rico et al (Rico and Villa, 1993), who demonstrated that serum TRAP concentration was increased after menopause and that such observation was inversely related to bone mass. Also in agreement with previous studies, our results also revealed an increase of OCN and CTX along with a decrease in ALP serum for the OVX group (da Paz et al, 2001; French et al, 2008; Garcia-Perez et al, 2006;Sims et al, 1996).…”

Ultrasound bone mass in paraplegic patients

“…Serum TRAP was quanti®ed in the Hitachi automated analyzer with a-naphthyl substrate, using a reagent from Boehringer Laboratories (Boehringer, Mannheim, Germany) that reacts speci®cally with the isoenzyme 5b synthesized by osteoclasts. 16 Twenty-four hour urinary calcium excretion was determined by atomic absorption spectroscopy using a Perkin Elmer Model 5000 spectrophotometer (Perkin Elmer, Norfolk, CT, USA). Blood samples were centrifuged and serum was stored at7208C until analyzed.…”