Elevated serum levels of the soluble form of IL-2 receptor ␣ (sIL-2R␣) have been correlated with a poor prognosis in a variety of different types of cancers. However, its biologic relevance remains unclear and controversial. In patients with follicular B-cell non-Hodgkin lymphoma (FL), we observed that serum sIL-2R␣ levels were elevated compared with controls and that elevated sIL-2R␣ levels before treatment were associated with a poor outcome. To explore the mechanism by which sIL-2R␣ may contribute to a poor prognosis in FL, we determined the effects of sIL-2R␣ on IL-2 signaling and found that the sIL-2R␣-IL-2 complex promoted T-cell differentiation toward to inhibitory T reg cells rather than T
IntroductionFollicular lymphoma (FL), the second most frequent type of non-Hodgkin lymphoma (NHL), is characterized by the presence of a significant number of normal T cells in the tumor microenvironment that have a substantial impact on antitumor immunity and patient outcome. 1,2 Previous studies have shown that the type of T cell-mediated immune response, which is regulated by the cytokine milieu, influences antitumor immunity thereby impacting patient outcome in FL. [3][4][5] Recent studies have highlighted the significance of CD4 ϩ CD25 ϩ Foxp3 ϩ regulatory T (T reg ) cells in the immune response and revealed the important role of T reg cells in the regulation of antitumor immunity. In FL, intratumoral T reg cells are present in significant numbers in biopsy specimens and markedly inhibit the proliferation and cytokine or granule production of intratumoral CD4 ϩ and CD8 ϩ T cells. [6][7][8] Lymphoma B cells play an important role in skewing the balance between T reg and IL-17-secreting T helper 17 (T H 17) cells resulting in the establishment of a profoundly inhibitory tumor microenvironment. 9 IL-2, a cytokine originally identified as a T-cell growth factor, plays a key role in the development, homeostasis, and function of T reg cells. IL-2 is essential in the development of T reg cells in the thymus, [10][11][12] and in the absence of IL-2, T reg cells cannot survive or expand in the thymus or in the periphery. [13][14][15][16] Furthermore, IL-2 is directly required for T reg cell function, and in its absence, T reg cells fail to suppress T-cell proliferation. 17,18 In B-cell NHL, IL-2 promotes T reg cell and inhibits T H 17 cell development, which is one of mechanisms explaining the presence of inhibitory tumor microenvironment in this disease. 9 IL-2 exerts its effect through binding to its receptor on cell surface. IL-2 receptor (IL-2R) is composed of 3 different subunits: ␣ (p55),  (p75), and ␥ (p64). The ␣ chain binds IL-2 with low affinity and is unable to initiate a signal in the absence of the other 2 subunits confirming that the integration of receptors for IL-2 signaling is essential. In addition to membrane receptors, several studies have demonstrated the existence of truncated, soluble form of IL-2R␣ that is generated exclusively by the proteolytic cleavage of membrane IL-2R␣. 19 It has been found t...