Serum responsive proteome reveals correlation between oxidative phosphorylation and morphogenesis in Candida albicans ATCC10231

Ahmed, Radfan; Kodgire, Santosh; Santhakumari, B.; Patil, Rajendra; Kulkarni, Mahesh J.; Zore, Gajanan

doi:10.1016/j.jprot.2018.06.018

Cited by 13 publications

(13 citation statements)

References 70 publications

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“…The highest increases in protein abundance were manifest by Asr1 (a ubiquitin ligase that modifies RNA polymerase II) and Asr2. These proteins were identified as adenylyl cyclase and stress responsive 58 in a study of transcriptional response of cAMP signalling in C. albicans , and are up-regulated following a range of other treatments including heat, osmotic 59 and weak acid stress 22 as well as in the response to 10% (v/v) fetal bovine serum in the medium 60 . The set of up-regulated proteins also include numerous stress proteins whose cognate genes are part of the core transcriptional response to stress in C. albicans 20 .…”

Section: Resultsmentioning

confidence: 99%

Specificity of the osmotic stress response in Candida albicans highlighted by quantitative proteomics

Jacobsen

Beynon

Gethings

et al. 2018

Sci Rep

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Stress adaptation is critical for the survival of microbes in dynamic environments, and in particular, for fungal pathogens to survive in and colonise host niches. Proteomic analyses have the potential to significantly enhance our understanding of these adaptive responses by providing insight into post-transcriptional regulatory mechanisms that contribute to the outputs, as well as testing presumptions about the regulation of protein levels based on transcript profiling. Here, we used label-free, quantitative mass spectrometry to re-examine the response of the major fungal pathogen of humans, Candida albicans, to osmotic stress. Of the 1,262 proteins that were identified, 84 were down-regulated in response to 1M NaCl, reflecting the decrease in ribosome biogenesis and translation that often accompanies stress. The 64 up-regulated proteins included central metabolic enzymes required for glycerol synthesis, a key osmolyte for this yeast, as well as proteins with functions during stress. These data reinforce the view that adaptation to salt stress involves a transient reduction in ribosome biogenesis and translation together with the accumulation of the osmolyte, glycerol. The specificity of the response to salt stress is highlighted by the small proportion of quantified C. albicans proteins (5%) whose relative elevated abundances were statistically significant.

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Section: Resultsmentioning

confidence: 99%

Specificity of the osmotic stress response in Candida albicans highlighted by quantitative proteomics

Jacobsen

Beynon

Gethings

et al. 2018

Sci Rep

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“…Recent proteomic studies have repeatedly revealed the presence of numerous putative moonlighting proteins in the candidal cell wall proteome [ 24 , 39 , 70 , 71 , 72 ], or in its subsets selected by special treatments such as pre-adsorption to host proteinaceous factors [ 73 , 74 , 75 ] or cell surface shaving by trypsin [ 24 , 56 , 72 , 76 , 77 ]. Classified according to their original intracellular function, the moonlighting proteins detected in the cell wall of C. albicans include: enzymes involved in evolutionally conserved central metabolic pathways such as: glycolysis and/or gluconeogenesis (phosphofructokinase (Pfk1), fructose-bisphosphate aldolase (Fba1), phosphoglycerate mutase (Gpm1), glyceraldehyde-3-phosphate dehydrogenase (Tdh3), enolase (Eno1) and pyruvate kinase (Cdc19)); fermentation (alcohol dehydrogenase (Adh1)); the pentose phosphate pathway (6-phosphogluconate dehydrogenase (Gnd1)); the Krebs cycle (aconitase (Aco1) and citrate synthase (Cit1)); factors associated with protein synthesis such as: ribosomal proteins (including Rpl3, Rpl5, Rpl9B, Rpl12, Rpl13, Rpl18, Rps22A and Rps31); elongation factors (elongation factor 2 (Eft2), elongation factor 3 (Eft3), translation elongation factor 1-alpha (Tef1) and elongation factor 1-beta (Efb1)); chaperones (Ssa1, Ssa2, Ssa4, Ssb1, Ssc1, Ssd1, Ssz1 and Kar2); enzymes involved in redox homeostasis (superoxide dismutase (Sod3), glutathione reductase (Glr1), peroxiredoxin (Tsa1)); [ 24 , 30 , 33 , 39 , 70 , 72 , 73 ].…”

Section: Atypical Proteinaceous Components Of Candidal Cell Wallmentioning

confidence: 99%

Moonlighting Proteins at the Candidal Cell Surface

et al. 2020

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The cell wall in Candida albicans is not only a tight protective envelope but also a point of contact with the human host that provides a dynamic response to the constantly changing environment in infection niches. Particularly important roles are attributed to proteins exposed at the fungal cell surface. These include proteins that are stably and covalently bound to the cell wall or cell membrane and those that are more loosely attached. Interestingly in this regard, numerous loosely attached proteins belong to the class of “moonlighting proteins” that are originally intracellular and that perform essentially different functions in addition to their primary housekeeping roles. These proteins also demonstrate unpredicted interactions with non-canonical partners at an a priori unexpected extracellular location, achieved via non-classical secretion routes. Acting both individually and collectively, the moonlighting proteins contribute to candidal virulence and pathogenicity through their involvement in mechanisms critical for successful host colonization and infection, such as the adhesion to host cells, interactions with plasma homeostatic proteolytic cascades, responses to stress conditions and molecular mimicry. The documented knowledge of the roles of these proteins in C. albicans pathogenicity has utility for assisting the design of new therapeutic, diagnostic and preventive strategies against candidiasis.

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“…e suspension of C. albicans expressing red fluorescent protein was prepared in RPMI 1640 medium with 10% (v/v) fetal bovine serum (FBS) to induce hyphae formation [26]. e suspension was then treated with MIC 80 concentrations of 1-3 at 37°C shaking.…”

Section: Albicans Filamentation Studymentioning

confidence: 99%

Antimicrobial Activity and DNA/BSA Binding Affinity of Polynuclear Silver(I) Complexes with 1,2-Bis(4-pyridyl)ethane/ethene as Bridging Ligands

Đurić

Vojnović

Andrejević

et al. 2020

Bioinorganic Chemistry and Applications

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1,2-Bis(4-pyridyl)ethane (bpa) and 1,2-bis(4-pyridyl)ethene (bpe) were used for the synthesis of polynuclear silver(I) complexes, {[Ag(bpa)]NO 3 } n (1), {[Ag(bpa) 2 ]CF 3 SO 3 . H 2 O} n (2) and {[Ag(bpe)]CF 3 SO 3 } n (3). In complexes 1-3, the corresponding nitrogencontaining heterocycle acts as a bridging ligand between two Ag(I) ions. In vitro antimicrobial activity of these complexes, along with the ligands used for their synthesis, was evaluated against the broad panel of Gram-positive and Gram-negative bacteria and fungi. e silver(I) complexes 1-3 showed selectivity towards Candida spp. and Gram-negative Escherichia coli in comparison to the other investigated bacterial strains, effectively inhibiting the growth of four different Candida species with minimal inhibitory concentrations (MICs) between 2.5 and 25 μg/mL and the growth of E. coli, with MIC value being 12.5 μg/mL. Importantly, complex 2 significantly reduced C. albicans filamentation, an essential process for its pathogenesis. Antiproliferative effect on the normal human lung fibroblast cell line MRC-5 was also evaluated with the aim of determining the therapeutic potential of the complexes 1-3. e interactions of these complexes with calf thymus DNA (ctDNA) and bovine serum albumin (BSA) were studied to evaluate their binding activities towards these biomolecules for possible insights on their mode of action.

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Serum responsive proteome reveals correlation between oxidative phosphorylation and morphogenesis in Candida albicans ATCC10231

Cited by 13 publications

References 70 publications

Specificity of the osmotic stress response in Candida albicans highlighted by quantitative proteomics

Specificity of the osmotic stress response in Candida albicans highlighted by quantitative proteomics

Moonlighting Proteins at the Candidal Cell Surface

Antimicrobial Activity and DNA/BSA Binding Affinity of Polynuclear Silver(I) Complexes with 1,2-Bis(4-pyridyl)ethane/ethene as Bridging Ligands

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