Serum response factor is modulated by the SUMO-1 conjugation system

Matsuzaki, Kei; Minami, Takeshi; Tojo, Masahiro; Honda, Yoshiomi; Uchimura, Yasuhiro; Saitoh, Hisato; Yasuda, Hideyo; Saijo, Nagahiro; Saya, Hideyuki; Nakao, Mitsuyoshi

doi:10.1016/s0006-291x(03)00910-0

Cited by 52 publications

(39 citation statements)

References 35 publications

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“…Expression of c-fos is controlled by serum response factor (SRF) and the ternary complex factor Elk-1, which form a transcription factor complex on the SRE element in the c-fos promoter. Both SRF and Elk-1 undergo SUMO modification and, consistent with a repressive role of SUMO in the AP-1 pathway, transcriptional activity of both SRF and Elk-1 is restrained by SUMOylation (Matsuzaki et al, 2003;Yang et al, 2003). This illustrates that SUMO can inhibit upstream effectors that control the availability of an AP-1 component.…”

Section: Ap-1 Pathwaymentioning

confidence: 59%

SUMO: a regulator of gene expression and genome integrity

2004

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Post-translational modification with the ubiquitin-like SUMO protein is involved in the regulation of many cellular key processes. The SUMO system modulates signal transduction pathways, including cytokine, Wnt, growth factor and steroid hormone signalling. SUMO frequently restrains the activity of downstream transcription factors in these pathways presumably by facilitating the recruitment of corepressors or mediating the assembly of repressor complexes. Additionally, evidence is accumulating that SUMO controls pathways important for the surveillance of genome integrity. SUMO regulates the PML/p53 tumour suppressor network, a key determinant in the cellular response to DNA damage. Moreover, proteins that maintain genomic stability by functioning at the interface between DNA replication, recombination and repair processes undergo SUMOylation. We will discuss some key findings that exemplify the role of SUMO in transcriptional regulation and genome surveillance.

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Section: Ap-1 Pathwaymentioning

confidence: 59%

SUMO: a regulator of gene expression and genome integrity

2004

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“…This result is not totally surprising because it was reported for other proteins. For example, serum response factor, Lef1͞ Tcf, and Smad3 are all sumoylated by PIASy, and their transcriptional activity is inhibited by PIASy coexpression, but in none of these cases does transcriptional repression require sumoylation (20,39,40). In the case of Lef1͞Tcf, interaction with PIASy results in accumulation within subnuclear compartments (20).…”

Section: Discussionmentioning

confidence: 99%

Modification of the erythroid transcription factor GATA-1 by SUMO-1

Collavin

Gostissa

Avolio

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

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The activity of transcription factors is tightly modulated by posttranslational modifications affecting stability, localization, and protein-protein interactions. Conjugation to SUMO is a reversible posttranslational modification that has been shown to regulate important transcription factors involved in cell proliferation, differentiation, and tumor suppression. Here, we demonstrate that the erythroid transcription factor GATA-1 is sumoylated in vitro and in vivo and map the single lysine residue involved in SUMO-1 attachment. We show that the nuclear RING finger protein PIASy promotes sumoylation of GATA-1 and dramatically represses its transcriptional activity. We present evidence that a nonsumoylatable GATA-1 mutant is indistinguishable from the WT protein in its ability to transactivate a reporter gene in mammalian cells and in its ability to trigger endogenous globin expression in Xenopus explants. These observations open interesting questions about the biological role of this posttranslational modification of GATA-1.

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“…It should also be pointed out that SRF can be modified by phosphorylation, acetylation, and sumoylation, and posttranslationally modified SRF may well exhibit lineage-specific interactions with transcriptional coactivators and corepressors. [91][92][93][94] It is also apparent that SMC differentiation in any lineage requires epigenetic modifications of intact chromatin to enable SRF and its cofactors to gain access to important cis-regulatory sites on SMC target genes. Whether or not lineage-specific chromatin remodeling complexes are formed during vascular SMC development is an important direction for future study.…”

Section: Majeskymentioning

confidence: 99%

Developmental Basis of Vascular Smooth Muscle Diversity

Majesky¹

2007

ATVB

625

536

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Abstract-The origins of vascular smooth muscle are far more diverse than previously thought. Lineage mapping studies show that the segmental organization of early vertebrate embryos leaves footprints on the adult vascular system in the form of a mosaic pattern of different smooth muscle types. Moreover, evolutionarily conserved tissue forming pathways produce vascular smooth muscle from a variety of unanticipated sources. A closer look at the diversity of smooth muscle origins in vascular development provides new perspectives about how blood vessels differ from one another and why they respond in disparate ways to common risk factors associated with vascular disease. (Arterioscler Thromb Vasc

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Serum response factor is modulated by the SUMO-1 conjugation system

Cited by 52 publications

References 35 publications

SUMO: a regulator of gene expression and genome integrity

SUMO: a regulator of gene expression and genome integrity

Modification of the erythroid transcription factor GATA-1 by SUMO-1

Developmental Basis of Vascular Smooth Muscle Diversity

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