Serum resistance associated with virulence in Yersinia enterocolitica

Pai, Chik Hyun; DeStephano, L

doi:10.1128/iai.35.2.605-611.1982

Cited by 122 publications

(88 citation statements)

References 23 publications

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“…Previous reports have shown that Y. enterocolitica serotype 08 with the virulence plasmid has the ability to cause intestinal infection with persistent presence of viable yersiniae in the ileal contents, ileal tissue, and Peyer's patches [5,20]. We compared the virulent strain IP383 of serotype 09 and its plasmid-less derivative IP383p-for their abilities to survive in the Peyer's patches after oral challenge (Table 1).…”

Section: Survival Of Bacteria In the Peyer's Patchesmentioning

confidence: 99%

Chromosome-mediated resistance of Yersinia enterocolitica serotype O9 to intracellular killing by mouse peritoneal macrophages

Ruiz‐Bravo

1994

FEMS Immunology and Medical Microbiology

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The survival of Yersinia enterocolitica serotype O9 within mouse peritoneal macrophages was investigated. To evaluate the role of the virulence plasmid in the resistance to intracellular killing, an isogenic pair of virulent (plasmid-bearing) and avirulent (plasmid-less) O9 strains was used. The virulent strain was able to express plasmid-encoded outer membrane proteins and to colonize the Peyer's patches of orally infected mice. When mice were infected intraperitoneally, both strains were recovered at similar rates and over the same time from the peritoneal cavity. When in vitro assays were performed, both strains showed similar resistance to intracellular killing by monolayers of resident and inflammatory peritoneal macrophages. Previous opsonization of bacteria did not modify their survival within macrophage monolayers. We concluded that serotype O9 strains display a chromosome-mediated resistance to intracellular killing by mouse peritoneal macrophages. Moreover, macrophage resistance does not seem to be of importance for virulence of serotype O9 strains in mice.

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Section: Survival Of Bacteria In the Peyer's Patchesmentioning

confidence: 99%

Chromosome-mediated resistance of Yersinia enterocolitica serotype O9 to intracellular killing by mouse peritoneal macrophages

Ruiz‐Bravo

1994

FEMS Immunology and Medical Microbiology

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“…In a previously published communication on Y. enterocolitica ( 13), we reported the existence of a distinct MRHA, which was strictly correlated with ing (40-45 Mdal) derivative (P+), and its isogenic plasmid-cured mutant (P-), cultivated at 22 and 37 "C. All four strains show plasmid-associated OMPs expressed phenotypically only at 37 "C. Arrows indicate two prominent plasmid-associated OMPs, common to all strains and serogroups tested. Strains NY81-71 (03) and 3315 (0:9), unlike the 0:3 strains, show plasmid-independent OMPs (PIOMPs) which are expressed mainly at 22 "C. The abscissa indicates molecular weights in kilodaltons.…”

Section: Discussionmentioning

confidence: 80%

Outer Membrane Proteins and Plasmids in Different Yersinia Enterocolitica Serogroups Isolated from Man and Animals

Kapperud

Skarpeid

Solberg

et al. 1985

Acta Pathologica Microbiologica Scandinavica Series B: Microbio

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Human clinical isolates of Yersinia enterocolitica (serogroups 0:3, 0:8, and 0:9) harboured a 40–45 megadaltons plasmid, which was shared by strains isolated from cases of yersiniosis in animals (0:2 and 0:5) and from healthy swine (0:3 and 0:9). Possession of such plasmids was invariably correlated with expression of three temperature‐regulated characteristics related to the bacterial surface: when cultivated at 37 °C, all plasmid‐bearing strains, unlike their plasmid‐cured mutants, (i) underwent autoaggutination, (ii) produced a distinct mannose‐resistant haemagglutinin, and (iii) simultaneously synthesized ancillary outer membrane proteins (OMPs). None of these characters was expressed phenotypically at 22 °C. Dominant among the plasmid‐associated, temperature‐regulated OMPs was a high molecular weight protein of approximately 180 kilodaltons, which was found in strains of five different serogroups (0:2, 0:3, 0:5, 0:8, and 0:9) regardless of the source of isolation. Strains isolated from diseased animals and from healthy swine were quite similar, if not identical, to human clinical isolates with respect to these ancillary OMPs. In contrast to the plasmid‐associated OMPs expressed only at 37 °C, a number of plasmid‐independent OMPs were produced mainly at 22 °C.

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“…It was tested according to the method of Pai and DeStephano (1982). Bacterial cultures were washed twice in PBS and suspended to 5.0 · 10 3 CFU/ml in PBS.…”

Section: Serum Bactericidal Activitymentioning

confidence: 99%

Attenuation and Preserved Immunogenic Potential of Yersinia pseudotuberculosis Mutant Strains Evidenced in Oral Pig Model

Najdenski

Golkocheva-Markova

Kussovski

et al. 2009

Zoonoses and Public Health

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Experimental oral infection of pigs with a parental Yersinia pseudotuberculosis strain pIB102, serotype O:3 and two mutant isogenic strains - pIB155,DeltayopK and pIB44,DeltaypkA has been carried out. Clinical findings, microbiological and immunological parameters were examined in dynamics from day 7 to day 60 post-infection (p.i.). All types of infections ran asymptomatically, without hyperthermia, loss of appetite, etc. Experiments on the blood parameters demonstrated a transient leucocytosis with lymphocytosis and monocytosis better expressed after yopK infection. Even though pig is usually known as a reservoir of yersiniae, bacterial colonization was found in mesenterial lymph nodes and tonsils on day 7, respectively 14 p.i. with parental strain, and only in tonsils on day 14 p.i. with both mutant strains. The augmented sensitivity of mutants to the bactericidal effect of leukocytes and blood sera is the characteristic feature of attenuation in their pathogenicity, compared to the parental strain. Comparative in vitro experiments on the immune response and immunostimulating capacity of Y. pseudotuberculosis mutant strains verify their preserved immunogenic potential, predominantly in case of yopK. Hyperplasia and strong activation of the lymph tissue of Peyer's patches, mesenterial lymph nodes, tonsils and spleen of pigs challenged with both mutant strains were proved as immunomorphological rearrangements. The results obtained give the reason to claim that the genetically constructed yopK null mutant strain is significantly attenuated but is still immunogenic and has the potential for a live vaccine carrier strain.

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Serum resistance associated with virulence in Yersinia enterocolitica

Cited by 122 publications

References 23 publications

Chromosome-mediated resistance of Yersinia enterocolitica serotype O9 to intracellular killing by mouse peritoneal macrophages

Chromosome-mediated resistance of Yersinia enterocolitica serotype O9 to intracellular killing by mouse peritoneal macrophages

Outer Membrane Proteins and Plasmids in Different Yersinia Enterocolitica Serogroups Isolated from Man and Animals

Attenuation and Preserved Immunogenic Potential of Yersinia pseudotuberculosis Mutant Strains Evidenced in Oral Pig Model

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