Serum proteomic profiling of patients with amphetamine use disorder

Alasmari, Fawaz; Alsanea, Sary; Masood, Afshan; Alhazzani, Khalid; Alanazi, Ibrahim O.; Musambil, Mohthash; Alfadda, Assim A.; Alshammari, Musaad A.; Alasmari, Abdullah F.; Benabdelkamel, Hicham

doi:10.1016/j.drugalcdep.2020.108157

Cited by 1 publication

(11 citation statements)

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“…IPA analysis showed that FXR/RXR is activated in humans chronically exposed to cannabis. This is in agreement with our previous work showing that FXR/RXR activation is documented in patients diagnosed with amphetamine use disorder [ 23 ]. Therefore, FXR/RXR activation can play a critical role in the toxicological effects of abused drugs such as amphetamine and cannabis.…”

Section: Discussionsupporting

confidence: 94%

“…Acute phase responses are correlated to various diseases, including immunological diseases [ 41 ]. The acute phase response signaling was the most interesting enriched canonical pathway involved in patients with amphetamine use disorder as shown in our previous proteomic study [ 23 ]. Interestingly, THC exposure was found to increase the mortality in mice infected with Legionella pneumophila at least in part by altering the acute phase responses of proinflammatory cytokines, an effect was not observed with cannabinol and cannabidiol as well as a synthetic cannabinoid, CP 55,940 [ 42 ].…”

Section: Discussionmentioning

confidence: 83%

“…IPA analysis showed that LXR/RXR is activated in humans chronically exposed to cannabis. Our prior proteomic study showed that LXR/RXR activation is observed in humans exposed to amphetamine for chronic period of time [ 23 ]. This indicates that LXR/RXR activation is highly sensitive following exposure to amphetamine and cannabinoids.…”

Section: Discussionmentioning

confidence: 99%

“…In our study, atherosclerosis signaling has been found to be modulated in CUD patients. Atherosclerosis signaling was one of the top canonical pathways that are involved in protein–protein interactions in amphetamine use disorder patients [ 23 ]. Note that apolipoprotein AI and inflammatory pathways interact with atherosclerosis signaling [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

“…Several reports have determined the serum proteomic profiling of humans exposed to amphetamine analogs [ 21 , 22 ]. A recent study from our group identified differentially expressed proteins in the serum of individuals with amphetamine use disorder compared with a healthy control group [ 23 ]. Moreover, prior clinical proteomic studies utilized serum samples to determine the levels of proteins in patients who had developed neurodegenerative diseases [ 24 , 25 ], neurodevelopmental disorders [ 26 , 27 , 28 ], major depressive disorder, and bipolar disorders [ 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

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Serum Proteomic Analysis of Cannabis Use Disorder in Male Patients

et al. 2021

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Cannabis use has been growing recently and it is legally consumed in many countries. Cannabis has a variety of phytochemicals including cannabinoids, which might impair the peripheral systems responses affecting inflammatory and immunological pathways. However, the exact signaling pathways that induce these effects need further understanding. The objective of this study is to investigate the serum proteomic profiling in patients diagnosed with cannabis use disorder (CUD) as compared with healthy control subjects. The novelty of our study is to highlight the differentially changes proteins in the serum of CUD patients. Certain proteins can be targeted in the future to attenuate the toxicological effects of cannabis. Blood samples were collected from 20 male individuals: 10 healthy controls and 10 CUD patients. An untargeted proteomic technique employing two-dimensional difference in gel electrophoresis coupled with mass spectrometry was employed in this study to assess the differentially expressed proteins. The proteomic analysis identified a total of 121 proteins that showed significant changes in protein expression between CUD patients (experimental group) and healthy individuals (control group). For instance, the serum expression of inactive tyrosine protein kinase PEAK1 and tumor necrosis factor alpha-induced protein 3 were increased in CUD group. In contrast, the serum expression of transthyretin and serotransferrin were reduced in CUD group. Among these proteins, 55 proteins were significantly upregulated and 66 proteins significantly downregulated in CUD patients as compared with healthy control group. Ingenuity pathway analysis (IPA) found that these differentially expressed proteins are linked to p38MAPK, interleukin 12 complex, nuclear factor-κB, and other signaling pathways. Our work indicates that the differentially expressed serum proteins between CUD and control groups are correlated to liver X receptor/retinoid X receptor (RXR), farnesoid X receptor/RXR activation, and acute phase response signaling.

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Section: Discussionsupporting

confidence: 94%