Serum proteomic changes in adults with obstructive sleep apnoea

Abstract

SUMMARYTo examine whether differentially expressed proteins are present in the serum of patients with obstructive sleep apnoea (OSA), iTRAQ techniques (isobaric tags for relative and absolute quantification) were employed in a prospective study. Individuals were assigned to either a non-OSA control group (apnoea-hypopnoea index, AHI <5) or an OSA group (AHI ‡5). Blood samples were collected, aliquoted and frozen at )80°C. Protein digestion and tagging with iTRAQ4plex â and mass spectrometry analysis was then performed (MALDI TOF ⁄ TOF). Ten male subjects were included in the control group (age = 45 ± 9.7 years) and 30 male patients in the OSA group (age = 45 ± 10.7 years), the latter being then subdivided into three severity groups. A total of 103 proteins were identified with differential levels between patients with OSA and controls. Of these, 11 proteins were underexpressed and 19 were overexpressed in patients with OSA. C4BPA and thrombospondin were underexpressed in all three OSA severity groups. Among the overexpressed proteins, 13 were overexpressed in the mild OSA group, seven in the moderate group and five in the severe group. Analysis of interactions between the identified proteins revealed that protein alterations in OSA are primarily associated with derangements in lipid and vascular metabolic pathways. This study provides initial evidence that differential protein expression occurs in adults with OSA, and that such proteins change according to disease severity, and appear to primarily involve lipid and vascular metabolic pathways.

Serum from obstructive sleep apnea patients induces inflammatory responses in coronary artery endothelial cells

“…Serum proteomics has been conducted on only a limited basis, revealing potential increases in a number of known proteins, such as fibronectin and ApoB100 in OSA patients [45]. Another study utilizing 2D electrophoresis noted increased serum levels of haptoglobins and reduced levels of paraoxonase-1 in OSA patients [46].…”

The promise of translational and personalised approaches for paediatric obstructive sleep apnoea: an ‘Omics’ perspective

“…Similarly, a recent study revealed differential serum protein expression in adults with OSA. These proteins were primarily those involved in lipid and vascular metabolic pathways, and the changes varied according to disease severity 90. It is unclear how this line of investigation will evolve and whether exploration of the deep proteome in plasma samples will enable development of a cluster biomarker panel that offers robust and accurate diagnostic capabilities.…”

Discovery and verification of serum differential expression proteins for pulmonary tuberculosis

“…Thus, it has become a powerful analytical technique for discovering disease biomarkers and drug targets. This strategy has recently been employed successfully to identify new markers for certain diseases, such as adult obstructive sleep apnea [12], autoimmune diseases [13,14], head-and-neck cancer [15], lung Cancer [16], and hepatocellular carcinoma [17].…”