Serum proteomic-based analysis by iTRAQ of damp-heat impeding syndrome of rheumatoid arthritis

Sun, Zilin; Xu, Xiao; Zhou, Danping; Wang, Fuqiang; Xu, Zheng; Ji, Wei

doi:10.1016/j.eujim.2015.07.001

Cited by 5 publications

(8 citation statements)

References 44 publications

(54 reference statements)

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“…The database search yielded 48 papers, while an additional 176 papers were identified through other sources. After assessing these for eligibility and eliminating duplicates, 10 articles met the inclusion criteria (eight qualitative studies [ 16 , 18 , 19 , 22 , 23 , 24 , 25 ], one randomized clinical trial [ 26 ] and one non-randomized study [ 27 ]). Figure 1 illustrates the study selection process.…”

Section: Resultsmentioning

confidence: 99%

“…Sample sizes ranged from 20 to 398 participants [ 18 , 26 ]. Continued Western medication therapy was an exclusion criteria in four studies [ 16 , 18 , 19 , 24 ], while three studies were unclear about this aspect [ 16 , 17 , 22 , 25 ]. Three studies allowed participants receiving nonsteroidal anti-inflammatory drugs, corticosteroids, or both if they had been on stable doses [ 23 , 26 , 27 ].…”

Section: Resultsmentioning

confidence: 99%

“…The tongue may be red with a yellow coating and the pulse may be rapid. Other symptoms include fever, thirst, a flushed face, irritability, restlessness, constipation and deep-coloured urine [ 16 , 18 , 19 , 22 , 23 , 24 , 25 ].…”

Section: Resultsmentioning

confidence: 99%

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Understanding Chinese Medicine Patterns of Rheumatoid Arthritis and Related Biomarkers

Seca

Franconi

2018

Medicines

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Background: A considerable number of Rheumatoid Arthritis (RA) patients only experience side effects from treatment, with little to no actual pain relief. The combination of disease diagnosis in biomedicine and multi-disciplinary integrative approaches such as Chinese Medicine (CM), can help to identify different functional diagnosis of RA in the context of biomarker discovery. We aimed to analyse CM patterns in RA and their biomarker profiles. Methods: Four electronic databases (web of science, CINAHL, Scopus and PubMed) were searched. The reference list of all identified reports and articles were searched for additional studies. All study designs were included and no date limits were set. Studies were considered if they were published in English and explored the possible biomarkers profiles in RA patients, classified according to the American College of Rheumatology and categorized in CM as either cold, heat/hot or deficiency patterns. Methodological quality of included studies was assessed using checklists adapted from the ©Critical Appraisal Skills Programme by two independent reviewers. A narrative synthesis was conducted, using thematic analysis. Results: A total of 10 articles were included. The studies examined 77 healthy volunteers and 1150 RA patients categorized as cold, heat/hot or deficiency pattern and related biomarkers were identified individually or concomitantly. Conclusions: CM pattern differentiation based on clinical signs and symptoms showed a diverse range of biomolecules, proteins and genes from RA patients correlated well with cold, heat/hot or deficiency phenotype-based CM patterns and could be used as diagnostic biomarkers for early detection, disease monitoring and therapeutic targets.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Understanding Chinese Medicine Patterns of Rheumatoid Arthritis and Related Biomarkers

Seca

Franconi

2018

Medicines

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“…Bioinformatics analysis of the differentially expressed proteins (DEPs) was performed using the tools available from DAVID Bioinformatics Resources 6.8 (http://www.geneontology.org/). The DEPs were annotated by gene ontology (GO) analysis according to the biological process (BP) and molecular function (MF) categories (Sun et al, ). Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis was performed using the KEGG database (http://www.genome.jp/kegg/pathway.html) (Chu et al, ).…”

Section: Methodsmentioning

confidence: 99%

iTRAQ‐based mitochondrial proteome analysis of the molecular mechanisms underlying postharvest senescence of Zizania latifolia

et al. 2019

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To explore the molecular mechanisms underlying postharvest senescence of Zizania latifolia, the changes in the mitochondrial proteome of plants treated with or without (control) 1-methyleyelopropene and ethylene during storage at room temperature for 0, 3 and 6 days were investigated using isobaric tags for relative and absolute quantitation (iTRAQ) labeling combined with two-dimensional liquid chromatography-tandem mass spectrometry. A total of 1,390 proteins with two or more peptides were identified, of which 211 showed a significant (p < .05) change (at least twofold) in relative abundance. Monitoring the parallel reaction validated the reliability and accuracy of the iTRAQ results. Bioinformatics and functional analysis of these differentially expressed proteins (DEPs) revealed that postharvest senescence of Z. latifolia could be attributed to (a) strengthened pentose phosphate pathway, (b) imbalanced protein, amino acid, organic acid, and fatty acid metabolism, (c) disordered energy homeostasis, (d) exacerbated oxidative damage, (e) RNA degradation, (f) activation of the Ca 2+ , mitogen-activated protein kinase, and jasmonic acid signaling pathways, (g) programed cell death, (h) excessive biosynthesis of secondary metabolites, or (i) degradation of cell structure. Our findings provide integrated insight into the molecular mechanisms of postharvest senescence during storage as well as the DEPs that show promise as targets for controlling senescence-induced quality deterioration of Z. latifolia. Practical applicationsPostharvest senescence is the most important factor that causes fast quality deterioration of Zizania latifolia. The understanding of the processes leading to postharvest senescence of Z. latifolia is essential in enhancing the commercial value and extending the shelf life of the product. It is currently believed that the mitochondrial metabolism is closely related to postharvest senescence. For this, the changes of proteome in Z. latifolia mitochondria treated with or without (control) 1-MCP and ETH during storage at room temperature were investigated. Results showed that a variety of 2 of 20 | LUO et aL.

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“…Recent studies have tried to investigate the association of one symptom with one syndrome [such as quantitative facial color features with cold pattern (Mun et al, 2017 )] or understanding the syndrome from the view of genotypes-phenotypes interactions (Chung, 2014 ; Fraser et al, 2015 ). And the main strategies include: (1) conducting the metabonomic and proteomic research (Shi et al, 2014b ; Zou et al, 2014 ; Jiang et al, 2015 ; Sun et al, 2015 ); (2) constructing MiRNA-target network (Liao et al, 2016 ; Liu et al, 2017 ); (3) integrating the classical formulas or herb pair (Chen et al, 2016 ; Xu et al, 2016 ; Zhou et al, 2016 ; Yue et al, 2017 ); (4) analyzing compound–nature pairs from TCM via chemical space visualizations (Liang et al, 2013 ; Fu et al, 2017 ); (5) using compound-target-disease associations to reconstruct the biologically-meaningful networks based on systems pharmacology (Zhou and Wang, 2014 ). Moreover, in recent years, a growing number of studies have focused on the biological mechanisms underlying BS with IHD (Mao et al, 2004 ; Liu Y. et al, 2011 ; Chen, 2012 ; Hao et al, 2013 ; Huang et al, 2013 ; Su et al, 2013 ; Wang and Yu, 2013 ; Li et al, 2015 ), PS with IHD (Wang et al, 2009 ; Zhao, 2009 ; Fang et al, 2011 ; Kong et al, 2014 ) and PSCS with IHD (Zhang et al, 1995 , 1999 ; Liu et al, 2008 ; Bai and Song, 2012 ; Lin et al, 2014 ; Zhao L. et al, 2014 ; Ren et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Integrated Modules Analysis to Explore the Molecular Mechanisms of Phlegm-Stasis Cementation Syndrome with Ischemic Heart Disease

Yang

Jiang

et al. 2018

Front. Physiol.

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Background: Ischemic heart disease (IHD) has been the leading cause of death for several decades globally, IHD patients usually hold the symptoms of phlegm-stasis cementation syndrome (PSCS) as significant complications. However, the underlying molecular mechanisms of PSCS complicated with IHD have not yet been fully elucidated.Materials and Methods: Network medicine methods were utilized to elucidate the underlying molecular mechanisms of IHD phenotypes. Firstly, high-quality IHD-associated genes from both human curated disease-gene association database and biomedical literatures were integrated. Secondly, the IHD disease modules were obtained by dissecting the protein-protein interaction (PPI) topological modules in the String V9.1 database and the mapping of IHD-associated genes to the PPI topological modules. After that, molecular functional analyses (e.g., Gene Ontology and pathway enrichment analyses) for these IHD disease modules were conducted. Finally, the PSCS syndrome modules were identified by mapping the PSCS related symptom-genes to the IHD disease modules, which were further validated by both pharmacological and physiological evidences derived from published literatures.Results: The total of 1,056 high-quality IHD-associated genes were integrated and evaluated. In addition, eight IHD disease modules (the PPI sub-networks significantly relevant to IHD) were identified, in which two disease modules were relevant to PSCS syndrome (i.e., two PSCS syndrome modules). These two modules had enriched pathways on Toll-like receptor signaling pathway (hsa04620) and Renin-angiotensin system (hsa04614), with the molecular functions of angiotensin maturation (GO:0002003) and response to bacterium (GO:0009617), which had been validated by classical Chinese herbal formulas-related targets, IHD-related drug targets, and the phenotype features derived from human phenotype ontology (HPO) and published biomedical literatures.Conclusion: A network medicine-based approach was proposed to identify the underlying molecular modules of PSCS complicated with IHD, which could be used for interpreting the pharmacological mechanisms of well-established Chinese herbal formulas (e.g., Tao Hong Si Wu Tang, Dan Shen Yin, Hunag Lian Wen Dan Tang and Gua Lou Xie Bai Ban Xia Tang). In addition, these results delivered novel understandings of the molecular network mechanisms of IHD phenotype subtypes with PSCS complications, which would be both insightful for IHD precision medicine and the integration of disease and TCM syndrome diagnoses.

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Serum proteomic-based analysis by iTRAQ of damp-heat impeding syndrome of rheumatoid arthritis

Cited by 5 publications

References 44 publications

Understanding Chinese Medicine Patterns of Rheumatoid Arthritis and Related Biomarkers

Understanding Chinese Medicine Patterns of Rheumatoid Arthritis and Related Biomarkers

iTRAQ‐based mitochondrial proteome analysis of the molecular mechanisms underlying postharvest senescence of Zizania latifolia

Integrated Modules Analysis to Explore the Molecular Mechanisms of Phlegm-Stasis Cementation Syndrome with Ischemic Heart Disease

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