Serum Proteome Signatures of Anti-SARS-CoV-2 Vaccinated Healthcare Workers in Greece Associated with Their Prior Infection Status

Stamoula, Еleni; Sarantidi, Eleana; Dimakopoulos, Vasilis; Ainatzoglou, Alexandra; Dardalas, Ioannis; Papazisis, Georgios; Kontopoulou, Konstantina; Anagnostopoulos, Athanasios K.

doi:10.3390/ijms231710153

Cited by 3 publications

(1 citation statement)

References 42 publications

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“…Data from the present study were searched only against the manually reviewed entries of UniProtKB, so that the highest quality of the data is ensured. The Proteome Discoverer parameters were used as described before by Stamoula et al [ 14 ].…”

Section: Methodsmentioning

confidence: 99%

Egg White and Yolk Protein Atlas: New Protein Insights of a Global Landmark Food

Sarantidi,

Ainatzoglou,

Papadimitriou

et al. 2023

Foods

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(1) Background: The chicken egg is an animal product of great agronomic interest. The egg white and yolk constitute high-quality protein sources for humans with high digestibility and well-balanced amino acid profiles. Despite the egg white and yolk protein’s undisputed value, research to unravel their full proteome content and its properties is still ongoing. We aimed to exhaustively analyze the proteome of egg white and yolk by applying intrinsic proteomics and bioinformatics approaches in order to unravel the full protein potential of this landmark food. (2) Methods: A total of 45 freshly laid, unfertilized, chicken eggs were subjected to nanoLC-MS/MS Orbitrap analysis following a peptide pre-fractionation step. A comprehensive bioinformatics processing step was undertaken towards elucidating potential activities and roles of identified molecules. In parallel, the literature was mined concerning all reported egg white and yolk protein identifications. (3) Results: Our analysis revealed 371 and 428 new proteins, reported for the first time to be present in the egg white and yolk, respectively. From the bioactivity standpoint, egg white and yolk proteins showed high enrichment for antioxidant and anti-inflammatory processes, while exerting high relevance for the apoptosis and focal adhesion pathways. (4) Conclusions: Egg white and yolk proteins exert diverse and multifaceted properties. A total of 799 proteins were reported for the first time as being part of the egg and yolk. Our novel protein data enriched those already published in the literature and the first ever chicken egg white and yolk Protein Atlas, comprising 1392 protein entries, was generated. This dataset will provide a cornerstone reference for future studies involving egg proteins.

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Section: Methodsmentioning

confidence: 99%

Egg White and Yolk Protein Atlas: New Protein Insights of a Global Landmark Food

Sarantidi,

Ainatzoglou,

Papadimitriou

et al. 2023

Foods

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Differential Serum Proteomic Signatures between Acute Aortic Dissection and Acute Myocardial Infarction

et al. 2023

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Acute aortic dissection (AAD) and acute myocardial infarction (AMI) are both severe cardiovascular diseases that may cause sudden death. However, whether serum proteins are differentially expressed between AAD and AMI remains unclear. Here, we aimed to explore serum protein profiles between AAD and AMI patients. A total of 75 serum samples were collected, including AAD patients without AMI (n = 25), AMI patients without AAD (n = 25), and normal subjects (n=25). Protein identities and expression levels were assessed by LC-MS/MS analysis and a label-free quantitation method, respectively. After depletion of albumin and IgG, a total of 117 proteins with differential expression (fold change ≥2 or ≤−2.0, p < 0.05) were identified, of which 60 were upregulated and 57 were downregulated in AAD sera as compared to AMI sera. Bioinformatic analysis revealed that the differentially expressed serum proteins were mainly derived from exosomes and the extracellular space, and their molecular functions and biological processes were primarily involved in the activity of transporters and complements and the immune response. In addition, the serum level of Cadherin-5, an identified protein with significant regulation in AAD, was further evaluated by ELISA and the results showed that Cadherin-5 in AAD sera was higher that in AMI and healthy sera. Collectively, these findings reveal the differential serum protein profiles between AAD and AMI, which may reflect the divergent pathophysiological progression between the two cardiovascular diseases.

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The Impact of Serum/Plasma Proteomics on SARS-CoV-2 Diagnosis and Prognosis

D’Amato,

Grignano,

Iadarola

et al. 2024

IJMS

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While COVID-19’s urgency has diminished since its emergence in late 2019, it remains a significant public health challenge. Recent research reveals that the molecular intricacies of this virus are far more complex than initially understood, with numerous post-translational modifications leading to diverse proteoforms and viral particle heterogeneity. Mass spectrometry-based proteomics of patient serum/plasma emerges as a promising complementary approach to traditional diagnostic methods, offering insights into SARS-CoV-2 protein dynamics and enhancing understanding of the disease and its long-term consequences. This article highlights key findings from three years of pandemic-era proteomics research. It delves into biomarker discovery, diagnostic advancements, and drug development efforts aimed at monitoring COVID-19 onset and progression and exploring treatment options. Additionally, it examines global protein abundance and post-translational modification profiling to elucidate signaling pathway alterations and protein-protein interactions during infection. Finally, it explores the potential of emerging multi-omics analytic strategies in combatting SARS-CoV-2.

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Serum Proteome Signatures of Anti-SARS-CoV-2 Vaccinated Healthcare Workers in Greece Associated with Their Prior Infection Status

Cited by 3 publications

References 42 publications

Egg White and Yolk Protein Atlas: New Protein Insights of a Global Landmark Food

Egg White and Yolk Protein Atlas: New Protein Insights of a Global Landmark Food

Differential Serum Proteomic Signatures between Acute Aortic Dissection and Acute Myocardial Infarction

The Impact of Serum/Plasma Proteomics on SARS-CoV-2 Diagnosis and Prognosis

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