Serum proteome analysis of vivax malaria: An insight into the disease pathogenesis and host immune response

S, Ray; Kamath, Karthik Shantharam; Srivastava, Rajneesh; Raghu, Dinesh; Gollapalli, Kishore; Jain, Rekha; Gupta, Shipra V.; Ray, Sayantan; Taur, Santosh; Dhali, Snigdha; Gogtay, Nithya J; Thatte, Urmila M; Rapole, Srikanth; Patankar, Swati; Srivastava, Sanjeeva

doi:10.1016/j.jprot.2011.10.018

Cited by 46 publications

(52 citation statements)

References 58 publications

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“…A series of studies over the past decade has provided valuable information regarding the proteomic alterations in human host in falciparum and vivax malaria [10][11][12]20,21]. In previously reported studies, differential abundance of multiple serum proteins including haptoglobin, serum amyloid A, serum albumin, apolipoprotein A-1, apolipoprotein E, alpha-2-macroglobulin, and vitronectin has been described in adults suffering from non-severe falciparum malaria [13,20] (Table 2).…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of Plasmodium falciparum induced alterations in humans from different endemic regions of India to decipher malaria pathogenesis and identify surrogate markers of severity

Kumar

Bhave

et al. 2015

Journal of Proteomics

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Section: Discussionmentioning

confidence: 99%

Proteomic analysis of Plasmodium falciparum induced alterations in humans from different endemic regions of India to decipher malaria pathogenesis and identify surrogate markers of severity

Kumar

Bhave

et al. 2015

Journal of Proteomics

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“…244 Other differentially expressed proteins were apolipoproteins A and E, serum amyloids A and P, Hp, and ceruloplasmin. Pathway analyses supported that there were changes in: the acute-phase response signaling, the complement system, coagulation, hemostasis, and vitamin D metabolism.…”

Section: Malariamentioning

confidence: 98%

Protection against Heme Toxicity: Hemopexin Rules, OK?

Smith¹

2013

Handbook of Porphyrin Science

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“…The in-gel digestion of the recombinant expressed protein separated by SDS-PAGE was processed as described by Ray et al [17] with slight modifications. The gel piece was diced and destained in 100 μl of destaining solution (50 mM ammonium bicarbonate/acetonitrile of 1:1, v/v) with intermittent gentle vortexing until most of the Coomassie dye was removed.…”

Section: In-gel Trypsin Digestionmentioning

confidence: 99%

Cloning, expression and purification of resistance gene analogue RGPM 301 from pearl millet in Escherichia coli

Veena¹,

Melvin²,

Sekhar

et al. 2016

J App Biol Biotech

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Plants combat their pathogens with an array of defense responses. One of the key mechanisms involves products of resistance (R) genes which are responsible for recognition of effector molecules from pathogens and subsequent triggering of defense responses. Resistance gene analogues (RGAs) containing the specific conserved domains of R-genes are isolated from various plants using degenerate oligonucleotide primer based PCR approach. In an earlier study, RGPM 301 an RGA from pearl millet shown to be involved in resistance mechanism against downy mildew disease was isolated and characterized. In the present study, RGPM 301 containing an open reading frame (ORF) of 992 amino acids was cloned into pRSET A expression vector and expressed in Escherichia coli as a Hig-tag fusion protein. The recombinant RGA RGPM 301 was purified to near homogeneity using the Nickel-CL agarose column. Its molecular mass was found to be 120 kDa when separated on the SDS-PAGE which was confirmed by western blotting analysis using the anti-His antibody. The purified protein was subjected to in-gel trypsin digestion followed by mass spectrometric analysis for the confirmation of its identity. These findings facilitate further studies on the exact role of this RGA in the pearl millet downy mildew host pathogen system.

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Serum proteome analysis of vivax malaria: An insight into the disease pathogenesis and host immune response

Cited by 46 publications

References 58 publications

Proteomic analysis of Plasmodium falciparum induced alterations in humans from different endemic regions of India to decipher malaria pathogenesis and identify surrogate markers of severity

Proteomic analysis of Plasmodium falciparum induced alterations in humans from different endemic regions of India to decipher malaria pathogenesis and identify surrogate markers of severity

Protection against Heme Toxicity: Hemopexin Rules, OK?

Cloning, expression and purification of resistance gene analogue RGPM 301 from pearl millet in Escherichia coli

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