Serum protein electrophoretic pattern in piglets during the early postnatal period

Abstract

The pattern of serum proteins, the typical features of the electrophoretogram in newborn piglets and during their postnatal development is not completely described. Therefore, the aim of this study was to characterize the changes in serum protein electrophoretic pattern and features of the electrophoretograms during the early postnatal period. Significant changes during the monitored period were found in all evaluated parameters (P < 0.001). The most marked changes were observed mainly in the period before weaning. The concentrations of total proteins, albumin and γ-globulins were before colostrum intake low, γ-globulins represented the smallest proportion of protein fractions. The proportion of α1-globulins was after birth a dominant protein fraction. Significant increase of total proteins, α2-, β- and γ-globulins and decrease of α1-globulins was found 2 days after colostrum intake. The albumin and A/G values increased after birth gradually until weaning. After weaning a significant changes were found in absolute concentrations of total protein and albumin, and in relative values of β-globulin fractions. Presented results showed marked developmental alterations in the serum protein pattern in piglets along with the age. The study also brings new knowledge in the field of description of typical features of electrophoretograms in the observed period of piglet’s life.

Evaluation of the Differences in the Serum Protein Electrophoretic Pattern in Precolostral Serum of Farm Animal Neonates

“…The proportion of albumin was higher than 50% in all the evaluated ruminant species, but the study showed differences in its concentrations between calves, lambs, as well as goat kids. Although the measured values were comparable to those presented previously [29][30][31], comparison studies between different animal species were not yet performed. Variations in the amino acid composition of the albumin, structure and binding sites for ligands were observed among different animal species [32].…”

“…Furthermore, electrophoresis in newborn piglets showed a double peak in the albumin zone with two approximately similar lower peaks that were previously not presented in any other species. The second peak on the cathodal side of the albumin zone probably represents the so called postalbumin fraction and its shape and size diminishes with advancing age [31]. According to Lardinois and Page [37], postalbumin could be a fetal-specific protein that disappears with age.…”

Characterization of maternally derived antibody titers against porcine circovirus type 2 in serum of piglets and identification of piglets at elevated risk of infection

“…The ADWG was estimated by the regression model using the recorded body weights at four-time points. The 5-day serum total protein concentration was selected based on the assumption that albumin levels at this time point are in uenced by hepatic synthesis, while globulin levels (derived from colostrum) will still be present [20]. Assessment of the serum total protein concentration at this early time point was employed as a biomarker indicating the piglet's risk for infection by PCV2.…”

“…Serum IgG was determined as an indicator of piglet colostrum intake. Colostrum-derived IgG (in piglet serum) has been reported to peak at 2 days of age [20]; therefore, serum IgG concentrations were determined at 1 and 3 days of age in the present study. The assay was conducted using the bicinchoninic acid (BCA) protein assay (Thermo Fisher Scienti c, Inc., Rockford, IL, USA) [21].…”

Metabolomics Analysis Reveals the Potential Relationship Between Sow Colostrum and Neonatal Serum Metabolites in Different Pig Breeds

“…[ 33 ] The concentrations of serum proteins in newborn piglets are very low; therefore, adequate biosynthesis and transportation of proteins are essential for their survival of newborn piglets. [ 34 ] Bile secretion is a crucial biochemical process in newborn piglets, and bile acids (the main component of bile) play a central role in the emulsification of luminal fat and participate in the normal fetal development and growth of piglets. [ 35 ] In addition, the metabolites matching these pathways were mainly decreased in Duroc pigs compared to those in the TB and XB pigs.…”