Serum protein changes in breast cancer: a prospective study.

Pettingale, K. W.; Tee, D. E. H.

doi:10.1136/jcp.30.11.1048

Cited by 15 publications

(5 citation statements)

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“…Serum ApoCI has also been previously found to be decreased in breast cancer patients compared to healthy control groups [ 21 ]. ApoH or beta2 glycoprotein was recognized immunologically over 30 years ago as being increased in the serum of breast cancer patients [ 36 ], but the 3808 Da ApoH fragment that we found to be increased in breast cancer sera has not been reported previously.…”

Section: Discussionmentioning

confidence: 55%

Novel serum protein biomarker panel revealed by mass spectrometry and its prognostic value in breast cancer

et al. 2014

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IntroductionSerum profiling using proteomic techniques has great potential to detect biomarkers that might improve diagnosis and predict outcome for breast cancer patients (BC). This study used surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry (MS) to identify differentially expressed proteins in sera from BC and healthy volunteers (HV), with the goal of developing a new prognostic biomarker panel.MethodsTraining set serum samples from 99 BC and 51 HV subjects were applied to four adsorptive chip surfaces (anion-exchange, cation-exchange, hydrophobic, and metal affinity) and analyzed by time-of-flight MS. For validation, 100 independent BC serum samples and 70 HV samples were analyzed similarly. Cluster analysis of protein spectra was performed to identify protein patterns related to BC and HV groups. Univariate and multivariate statistical analyses were used to develop a protein panel to distinguish breast cancer sera from healthy sera, and its prognostic potential was evaluated.ResultsFrom 51 protein peaks that were significantly up- or downregulated in BC patients by univariate analysis, binary logistic regression yielded five protein peaks that together classified BC and HV with a receiver operating characteristic (ROC) area-under-the-curve value of 0.961. Validation on an independent patient cohort confirmed the five-protein parameter (ROC value 0.939). The five-protein parameter showed positive association with large tumor size (P = 0.018) and lymph node involvement (P = 0.016). By matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS, immunoprecipitation and western blotting the proteins were identified as a fragment of apolipoprotein H (ApoH), ApoCI, complement C3a, transthyretin, and ApoAI. Kaplan-Meier analysis on 181 subjects after median follow-up of >5 years demonstrated that the panel significantly predicted disease-free survival (P = 0.005), its efficacy apparently greater in women with estrogen receptor (ER)-negative tumors (n = 50, P = 0.003) compared to ER-positive (n = 131, P = 0.161), although the influence of ER status needs to be confirmed after longer follow-up.ConclusionsProtein mass profiling by MS has revealed five serum proteins which, in combination, can distinguish between serum from women with breast cancer and healthy control subjects with high sensitivity and specificity. The five-protein panel significantly predicts recurrence-free survival in women with ER-negative tumors and may have value in the management of these patients.

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Section: Discussionmentioning

confidence: 55%

Novel serum protein biomarker panel revealed by mass spectrometry and its prognostic value in breast cancer

et al. 2014

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“…These differences are seen preoperatively at the earliest time of clinical detection of a breast tumour and do not appear to be related to the age difference between the groups. Raised serum ceruloplasmin has been reported previously in patients with both cancer and chronic inflammatory diseases (8,9), but an increase in P,, glycoprotein does not appear to have been reported before. In previous studies the levels have been either unchanged (3) or reduced (8).…”

Section: And Muneirmentioning

confidence: 73%

Must plagiarism thrive?

1980

BMJ

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“…In addition, we have found diverse changes in other serum proteins in our patients with breast cancer (Pettingale and Tee, 1977) …”

Section: Discussionmentioning

confidence: 99%

Prognostic value of serum levels of immunoglobulins (IgG, IgA, IgM and IgE) in breast cancer: a preliminary study

Pettingale¹,

Merrett²,

Tee³

1977

Br J Cancer

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Summary.-One hundred and sixty women admitted for breast tumour biopsy to the King's College Hospital group have been followed sequentially for 2 years. Sixty-nine women had early operable breast cancer and 91 had benign breast disease. All these women had serum immunoglobulin IgG, IgA, IgM and IgE levels measured preoperatively and postoperatively at 3 months, 1 year and 2 years. No differences were found in any of the serum immunoglobulin levels between the two groups at any time. There was, however, a positive correlation between the extent of metastatic breast cancer and the serum level of various immunoglobulins, particularly IgA. There was no evidence that routine postoperative radiotherapy influenced the levels of serum immunoglobulins. The findings suggest a secondary defence reaction against increasing tumour load, and do not support the theory of an early immune defect in immunoglobulin metabolism which could play a part in the pathogenesis of breast cancer. Although there is no diagnostic value in measuring the levels of serum immunoglobulins in patients with breast tumours, there may be some value in following the levels in cancer patients, as a guide to subclinical spread of the disease.

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Serum protein changes in breast cancer: a prospective study.

Cited by 15 publications

References 10 publications

Novel serum protein biomarker panel revealed by mass spectrometry and its prognostic value in breast cancer

Novel serum protein biomarker panel revealed by mass spectrometry and its prognostic value in breast cancer

Must plagiarism thrive?

Prognostic value of serum levels of immunoglobulins (IgG, IgA, IgM and IgE) in breast cancer: a preliminary study

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