Serum prolidase activity and oxidant–antioxidant status in children with chronic hepatitis B virus infection

Abstract

BackgroundChronic hepatitis B (CHB) is a global health problem that can result in serious complications associated with collagen degradation. Prolidase is a specific imidodipeptidase that plays an important role in the breakdown of collagen. The aim of this study was to investigate prolidase activity and oxidant–antioxidant status in children with CHB.MethodsThis prospective case control study includes 38 patients with CHB, 31 patients with inactive hepatitis B (IHB), and 29 healthy matched control subjects. Serum prolidase enzyme activity (SPEA), total antioxidant capacity (TAC), total oxidative activity (TOA), and malondialdehyde (MDA) level were measured and oxidative stress index (OSI) was calculated for each group.ResultsPatients with CHB had significantly higher SPEA levels (207.82 ± 186.80 IU/L) than did the controls (58.6 ± 38.1 IU/L) and IHB patients (67.1 ± 39.9) (p < 0.001). CHB patients also had significantly higher TOA (45.0 ± 19.9 vs. 29.4 ± 11.7 (μmolH2O2 Eq./L), p = 0.005), OSI (33.1 ± 21.4 vs. 17.5 ± 10.2, p = 0.002) and MDA (13.4 ± 4.0 vs. 7.8 ± 2.6 μm/L, p < 0.001) values compared with the controls. TOA (32.0 ± 10.0) and OSI (15.4 ± 11.0) values of IHB patients were significantly lower than those of CHB patients (p < 0.05). SPEA had significant correlations with HBV- DNA and ALT values (r =0.514 and r =0.454, p < 0.001).ConclusionOur results suggest that prolidase activity can be considered as a reliable marker for CHB and increased oxidative stress appears to be related to chronicity of the disease.

Hepatitis B and its Relationship With Oxidative Stress

“…These studies mostly measured MDA as an oxidation marker, which was significantly higher in patients with hepatitis B infection compared to healthy individuals (Table 1); a marker that was positively correlated to alanine aminotransferase (ALT) (23-25, 30, 31, 41), HBV-DNA (23, 32), total and direct bilirubin (28), serum bcl-2 (this study combined hepatitis B and C patients) (47), gamma-glutamyl transpeptidase (GGT) (31), serum iron/ferritin (44), and liver ultrasonography findings (coarse texture) (28) in some studies. Although another study demonstrated a significant increase in the MDA levels of chronic hepatitis B patients, it did not find a significant difference between inactive HBsAg carriers and controls (48).…”

The relationships among the levels of oxidative and antioxidative parameters, FEV1 and prolidase activity in COPD

“…Duygu et al 21 showed that prolidase activity and oxidative stress increase in patients with hepatitis C virus (HCV) infection and that the prolidase enzyme and oxidative damage may be associated with the HCV infection becoming chronic. However, Şen et al 22 reported a significant increase in prolidase activity in children with chronic hepatitis.…”

Impact of morbid obesity and bariatric surgery on antioxidant/oxidant balance of the unstimulated and stimulated human saliva

“…TAS is used to assess the overall antioxidant status; similarly, TOS is used to estimate the level of oxidants. One of the most important parameters evaluating oxidative stress is oxidative status index (OSI), which provides an objective assessment of the relationship between the antioxidant barrier and the ROS level and is a useful intensity oxidative stress marker .…”

“…The level of phenolic and polyphenolic compounds (pPh) was determined by the colorimetric method of Folin‐Ciocalteu's reagent (FCR) . Lipid peroxidation products – malondialdehyde (MDA) – were determined by measuring the content of MDA with thiobarbituric methods (TBARS) according to the Buege and Austa method .The oxidative stress index (OSI) was calculated from the TOS/TAS × 100 formula . The protein concentration was assessed using the bicinchoninic acid method (BCA) (bovine serum albumin as a standard) (Thermo Scientific PIERCE BCA Protein Assay Kit, Rockford, IL, USA).…”