Serum Pro-Oxidant-Antioxidant Balance, Advanced Oxidized Protein Products (AOPP) and Protein Carbonyl in Patients With Stroke

Pouya, Vajiheh Toloue; Hashemy, Seyed Isaac; Shoeibi, Ali; Tirkani, Abolfazl Nosrati; Tavallaie, Shima; Avval, Farnaz Zahedi; Soukhtanloo, Mohammad; Mashkani, Baratali; Alamdari, Daryoush Hamidi

doi:10.17795/rijm38203

Cited by 3 publications

(3 citation statements)

References 49 publications

(53 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other authors measured PC levels within 24 hours of symptom onset and obtained conflicting results. 29,30,32,49 At 3 months, PC levels were lower than in the acute phase, which agrees with other studies. 29,50 Additionally, they were lower among thrombolysed compared with untreated patients.…”

Section: Discussionsupporting

confidence: 92%

See 1 more Smart Citation

Protein Carbonylation Contributes to Prothrombotic Fibrin Clot Phenotype in Acute Ischemic Stroke: Clinical Associations

Błaż,

Natorska,

Bembenek

et al. 2023

Stroke

View full text Add to dashboard Cite

BACKGROUND: Acute ischemic stroke (AIS) is associated with enhanced oxidative stress and unfavorably altered fibrin clot properties. We investigated determinants of plasma protein carbonylation (PC) in AIS, its impact on the prothrombotic state, and prognostic value during follow-up. METHODS: We included 98 consecutive AIS patients aged 74±12 years (male:female ratio, 50:48 [51%:49%]) at the Neurology Center in Warsaw, Poland, between January and December 2014. As many as 74 (75.5%) patients underwent thrombolysis, and 24 were unsuitable for thrombolysis. We determined plasma PC, along with thrombin generation, fibrin clot permeability, and clot lysis time on admission, at 24 hours, and 3 months. Stroke severity was assessed using the National Institutes of Health Stroke Scale and stroke outcome with the modified Rankin Scale. Hemorrhagic transformation was assessed on the computed tomography scan within 48 hours from the symptom onset, while stroke-related mortality was evaluated at 3 months. RESULTS: On admission, PC levels (median, 4.61 [3.81–5.70] nM/mg protein) were associated with the time since symptom onset (r=0.41; P <0.0001) and with the National Institutes of Health Stroke Scale score ( P =0.36; P =0.0003). Higher PC levels on admission correlated with denser fibrin clot formation and prolonged clot lysis time but not with thrombin generation. In thrombolysed patients, lower PC levels were observed after 24 hours (−34%) and at 3 months (−23%; both P <0.001). PC levels at baseline and after 24 hours predicted the modified Rankin Scale score >2 at 3 months (OR, 1.90 [95% CI, 1.21–3.00]; OR, 2.19 [95% CI, 1.39–3.44], respectively). Higher PC at baseline predicted hemorrhagic transformation of stroke (OR, 1.95 [95% CI, 1.02–3.74]) and stroke-related mortality (OR, 2.02 [95% CI, 1.08–3.79]), while higher PC at 24 hours predicted solely stroke-related mortality (OR, 2.11 [95% CI, 1.28–3.46]). CONCLUSIONS: Elevated plasma PC levels in patients with AIS, related to prothrombotic fibrin clot properties, are associated with stroke severity. Thrombolysis reduces the extent of PC. The current study suggests a prognostic value of PC in AIS.

show abstract

Section: Discussionsupporting

confidence: 92%

“…27 Several oxidative stress markers have been evaluated in the setting of AIS 28 ; however, data on PC in AIS are scarce. PC levels are increased in patients with stroke 29,30 and contribute to unfavorable functional outcome and mortality, 31 as well as to poststroke depression, 32 in thrombolysed AIS patients compared with healthy controls.…”

mentioning

confidence: 99%

Protein Carbonylation Contributes to Prothrombotic Fibrin Clot Phenotype in Acute Ischemic Stroke: Clinical Associations

Błaż,

Natorska,

Bembenek

et al. 2023

Stroke

View full text Add to dashboard Cite

show abstract

“…The prooxidant-antioxidant balance (PAB) was performed by the method described by Toloue Pouya V. et al [17], modified by Pantea V. et al [18]. The method is based on the capacity of the free radicals, peroxides and antioxidants, contained in the blood sample, to interact with the TMB (3,3′,5,5′-tetramethylbenzidine) or TMB cation, that will determine changes of the solution color.…”

Section: Methodsmentioning

confidence: 99%

Harmfulness of prooxidants in bronchopulmonary dysplasia in preterm children

Ceahlau,

Selevestru,

Tagadiuc

et al. 2023

MJHS

View full text Add to dashboard Cite

Introduction. Oxidative stress can be defined as the imbalance of the redox state of a certain system including living one (organelle, cell, organ/tissue), which excessively produces reactive oxygen and/or reactive nitrogen species (ROS/RNS) that exceed the capacity of the antioxidant defense system, which have the ability to slow down or even prevent the oxidative damage of macromolecules. Oxidative stress is a pathogenic mechanism of a large variety of diseases, including pulmonary one. Material and methods. 81 preterm born children included in the study were divided into the main group – preterm children with bronchopulmonary dysplasia (BPD), and the control group – preterm children without BPD. The comparison groups were prospectively evaluated clinical, instrumental and laboratory (TPA, prooxidant-antioxidant balance, nitric oxide metabolistes and MDA). Data were statistically analyzed using Microsoft Excel, MedCalc and SPSS and Contingency Table Analysis as a way to evaluate the performance of a diagnostic test. Results. In preterm children with BPD were found to be decreased by 29% (p < 0.001) the prooxidant-antioxidant balance (PAB) and the nitric oxide metabolistes (NO) level by 12% (p < 0.001) compared to children in the control group. The assessment of tissue oxidative damage markers revealed a significant 62% (p < 0.001) increase in malonic dialdehyde (MDA) content and a 4.86-fold (p < 0.001) increase in total prooxidant activity (TPA) in children with bronchopulmonary dysplasia compared to children in the control group. Our study confirms that TPA, PAB, MDA and NO values are reliable markers of hypoxic tissue damage at children with bronchopulmonary dysplasia and can be recommended for assessing the intensity of oxidative stress. Conclusions. Pulmonary bronchodysplasia is characterized by the imbalance of prooxidant-antioxidant processes with the exacerbation of prooxidant ones that trigger the oxidative/nitrosative stress and the deterioration of vital chemical compounds.

show abstract

Determination of pro-oxidant-antioxidant balance (PAB) assay in mothers with spontaneous abortion

Ghasemi

Kamali

Shokrpour

2021

View full text Add to dashboard Cite

Oxidative stress has been identified to play a vital role in the pathogenesis of spontaneous abortion were characterized as an imbalance between the generation of pro-oxidants, free radicals, and reactive species, and enzymatic and non-enzymatic antioxidant defenses in favor of the former. In the present study, the pro-oxidant-antioxidant balance was assessed in women with spontaneous abortion and compared with healthy age-matched controls. A group of 50 females with spontaneous abortion were considered patients, and a group of age-matched healthy pregnant women was considered controls. The pro-oxidant-antioxidant balance (PAB) assay was carried out on participants' serum samples. The mean age of the spontaneous abortion group was 30.84 ± 3.82, and controls were 26.53 ± 4.05 years. The obtained PAB values were 236.74 ± 11.37 HK and 148.69 ± 76.50 HK in patients and controls. Our results demonstrate the significant rise of PAB values in subjects with spontaneous abortion compared to healthy controls (p< 0.0001). Our study showed that the PAB values might be involved in the termination of spontaneous abortion.

show abstract

Serum Pro-Oxidant-Antioxidant Balance, Advanced Oxidized Protein Products (AOPP) and Protein Carbonyl in Patients With Stroke

Abstract: Background: Stroke is the second cause of death and disability in the world, and oxidative stress which could be considered as a prognostic factor in patients for prevention, follow up and determination of stroke's severity has a conceivable role in neural damage.

Cited by 3 publications

References 49 publications

Protein Carbonylation Contributes to Prothrombotic Fibrin Clot Phenotype in Acute Ischemic Stroke: Clinical Associations

Protein Carbonylation Contributes to Prothrombotic Fibrin Clot Phenotype in Acute Ischemic Stroke: Clinical Associations

Harmfulness of prooxidants in bronchopulmonary dysplasia in preterm children

Determination of pro-oxidant-antioxidant balance (PAB) assay in mothers with spontaneous abortion

Contact Info

Product

Resources

About