Serum Pigment Studies in Newborn Infants<subtitle>I. Erythroblastosis Fetalis</subtitle>

Cotrimoxazole and neonatal kernicterus: a review

“…Plasma concentrations of bilirubin exceeding 20 mg/100 mL are considered levels at which kernicterus can occur (Crosse et al, 1955; Tuttle, 1955). In an in vitro study with sera from 20 newborn infants with high bilirubin content (18–28 mg%) using ultrafiltration and dialysis techniques, it was found that sulfisoxazole addition (clinically relevant blood levels) to these sera could uncouple protein-bound bilirubin and allow it to pass through the semipermeable membrane (Odell, 1959).…”

Studies in Kernicterus. I. The Protein Binding of Bilirubin*

“…The association between serum bilirubin concentration and kernicterus (2,3), the isolation of bilirubin from kernicteric brains (4,5), and the toxic effect of bilirubin in vitro upon oxygen uptake (6) and oxidative phosphorylation (7) are further evidence of a relationship between bilirubinemia and kernicterus. Plasma concentrations of bilirubin exceeding 20 mg. per cent are considered levels above which kernicterus occurs, although many exceptions exist (8,9), particularly in the premature infant (10). Of particular importance was the observation (11) that kernicterus occurred more frequently in premature infants given sulfisoxazole than in control infants despite lower plasma bilirubin concentrations (12).…”

Proceedings of the Biochemical Society

“…of 1 5% ammonium sulphamate to the Van den Bergh reagent, and secondly by addition of sodium azide immediately before filtration (Patterson, Swale & Maggs, 1952). Tuttle (1955) has shown that in erythroblastosis traces of other haem-pigments in the sera may be significant; these, and any products of haemolysis incidental to obtaining the blood specimens, affect the final measurement of azo-bilirubin colour. From an examination of the absorption curves of our final solutions, a simple correction formula for obtaining the true extinction value has been devised.…”