Serum pharmacochemistry for tracking bioactive components by UPLC-Q-TOF-MS/MS combined chromatographic fingerprint for quality assessment of Sanziguben Granule

Zhang, Chenxue; Lian, Ruixin; Mahmoodurrahman, Mohammed; Lai, Sisi; Zhao, Zhongxiang; Yu, Yang

doi:10.1016/j.jchromb.2016.07.022

Cited by 23 publications

(10 citation statements)

References 34 publications

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“…Traditionally, isolation and puri cation of chemical constituents mediated by solvent extraction, column chromatography, and/or combined with pharmacodynamic tests are time-consuming and ine cient. To date, based on serum pharmacochemistry, research on the components in the serum could provide an effective methodology to identify active constituents in vivo from Traditional Chinese medicine (TCM) [13,14]. Additionally, network pharmacology has been widely used to explore the molecular mechanisms of TCM, based on system biology and multidirectional pharmacology [15,16].…”

Section: Introductionmentioning

confidence: 99%

WITHDRAWN: Network Pharmacology-based to Investigate Pharmacological Mechanisms of Active Ingredients in Gegen Qinlian Pill to Ameliorate Irinotecan-induced Diarrhea

Cheng

Yang

et al. 2020

Preprint

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Background: The application of natural extracts or compounds derived from Chinese medicine is deemed to a promising strategy to prevent irinotecan (CPT-11)-induced gut toxicity. Based on the protective activity of Gegen Qinlian decoction in our previous study, we speculated that Gegen Qinlian Pill (GQP) would exhibit similar therapeutic effects. However, the effective material basis and the potential mechanisms underlying the effect of GQP in the treatment of diarrhea induced by CPT-11 have not been fully elucidated. Herein, we primarily investigated amelioration effect of GQP on the CPT-11 induced gut toxicity, and further explore its anti-diarrhea mechanism.Methods: Firstly, the protective effect of GQP towards alleviating diarrhea in mice following CPT-11 administration was investigated. Furthermore, the effective ingredients of GQP in serum sample of mice by HPLC-Q-TOF-MS analysis was obtained. Next, based on these active components, an interaction network linking “compound-target-pathway” was established. Finally, a predicted mechanism within vivo validation of GQP based on Gene Ontology (GO) functional and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were obtained. Results: A total of 19 chemical compounds derived from the GQP were identified in its serum sample. Then, an interaction network linking “compound-target-pathway” was established to illuminate the interaction between these components absorbed into serum and their targets for diarrhea. GQP exerted a curative effect on diarrhea and diarrhea-related diseases by targeting different targets, regulating inflammation, oxidative stress, and proliferation processes. Conclusion: Taken together, this study provides a novel and scientific strategy to discover the potential effective constituents of herbs or herbal formula, and elucidated basic pharmacological effects and underlying mechanisms of GQP in the treatment of CPT-11 induced gut toxicity.

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Section: Introductionmentioning

confidence: 99%

WITHDRAWN: Network Pharmacology-based to Investigate Pharmacological Mechanisms of Active Ingredients in Gegen Qinlian Pill to Ameliorate Irinotecan-induced Diarrhea

Cheng

Yang

et al. 2020

Preprint

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“…Recently, a reasonable hypothesis has been proposed that only the components of herbs absorbed into blood and circulated in the body have the opportunity to exert pharmaceutical efficacy, while the unabsorbed components cannot play a role in the body [28]. Therefore, the absorbed components have the potential to become bioactive components of herbs.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, the absorbed components have the potential to become bioactive components of herbs. Serum pharmacochemistry mainly focuses on the analysis of dosed serum by UHPLC‐MS/MS, which has been applied to searching or tracking absorbed components of medicinal plants and assessing herbal quality [28–30].…”

Section: Resultsmentioning

confidence: 99%

Discovery and validation of quality markers of Fructus Aurantii against acetylcholinesterase using metabolomics and bioactivity assays

Wang

Zhou

Dai

et al. 2021

J of Separation Science

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Fructus Aurantii is a traditional medicated diet in East Asia. To determine the underlying chemical markers responsible for the quality and efficacy of Fructus Aurantii, a sensitive metabolomic method was applied to distinguish Fructus Aurantii in Jiangxi Province from other two geographical locations (Hunan Province and Chongqing City) in China. In the present study, multivariate analyses were adopted to compare chemical compositions in 21 batches of Fructus Aurantii samples. Among three geographical origins, 23 differential compounds were structurally identified. Serum pharmacochemistry exhibited that 22 components could be detected in rat serum. Six differential and absorbed components were selected as six potential markers. Statistical analysis revealed that the content of six markers varied widely in three origins of Fructus Aurantii. Six differential and absorbed components were evaluated further by biological activity. Neohesperidin, naringin, and meranzin showed inhibitory effect on acetylcholinesterase that regulates gastrointestinal motility in vitro and in silico, suggesting that these three components may be determined as the active biomarkers of Fructus Aurantii. These findings demonstrate the potential of biomarkers for identification and quality control of Fructus Aurantii.

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“…Traditional Chinese herbal formulae generally contain numerous compounds; consequently, data on their pharmacokinetics properties, mechanisms of action, and safety profiles are frequently limited. Therefore, determination of the constituent compounds of such medicines, understanding the chemical basis of their activities, and establishment of quality control procedures have become a focus of intensive research, raising particular challenges ( Zhang et al, 2016 ). Serum pharmacochemistry of traditional Chinese herbs is a method used to screen for effective substances by identification and analysis of constituents migrating to the blood after oral administration ( Ma et al, 2017 ), since those herbal compounds that are absorbed into the circulation can be considered potential bioactive compounds associated with therapeutic effects.…”

Section: Introductionmentioning

confidence: 99%

Bu-Shen-Huo-Xue Decoction Ameliorates Diabetic Nephropathy by Inhibiting Rac1/PAK1/p38MAPK Signaling Pathway in High-Fat Diet/Streptozotocin-Induced Diabetic Mice

et al. 2020

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Diabetic nephropathy (DN), a leading cause of end-stage renal disease, is associated with high morbidity and mortality rates worldwide and the development of new drugs to treat DN is urgently required. Bu-Shen-Huo-Xue (BSHX) decoction is a traditional Chinese herbal formula, made according to traditional Chinese medicine (TCM) theory, and has been used clinically to treat DN. In the present study, we established a high-fat diet/streptozotocin-induced diabetic mouse model and treated the mice with BSHX decoction to verify its therapeutic effects in vivo. Ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was applied to analyze the chemical composition and active compounds of BSHX decoction. Markers of podocyte epithelial-mesenchymal transition and the Rac1/PAK1/p38MAPK signaling pathway were evaluated to investigate the mechanism underlying function of BSHX decoction. BSHX decoction effectively alleviated diabetic symptoms, according to analysis of the renal function indicators, serum creatinine, blood urea nitrogen, serum uric acid, and urinary albumin excretion rate, as well as renal histopathology and ultrastructural pathology of DN mice. We identified 67 compounds, including 20 likely active compounds, in BSHX decoction. The podocyte markers, nephrin and podocin, were down-regulated, while the mesenchymal markers, α-SMA and FSP-1, were up-regulated in DN mouse kidney; however, the changes in these markers were reversed on treatment with BSHX decoction. GTP-Rac1 was markedly overexpressed in DN mice and its levels were significantly decreased in response to BSHX decoction. Similarly, levels of p-PAK1 and p-p38MAPK which indicate Rac1 activation, were reduced on treatment with BSHX decoction. Together, our data demonstrated that BSHX decoction ameliorated renal function and podocyte epithelial-mesenchymal transition via inhibiting Rac1/PAK1/p38MAPK signaling pathway in high-fat diet/streptozotocin-induced diabetic mice. Further, we generated a quality control standard and numerous potential active compounds from BSHX decoction for DN.

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Serum pharmacochemistry for tracking bioactive components by UPLC-Q-TOF-MS/MS combined chromatographic fingerprint for quality assessment of Sanziguben Granule

Cited by 23 publications

References 34 publications

WITHDRAWN: Network Pharmacology-based to Investigate Pharmacological Mechanisms of Active Ingredients in Gegen Qinlian Pill to Ameliorate Irinotecan-induced Diarrhea

WITHDRAWN: Network Pharmacology-based to Investigate Pharmacological Mechanisms of Active Ingredients in Gegen Qinlian Pill to Ameliorate Irinotecan-induced Diarrhea

Discovery and validation of quality markers of Fructus Aurantii against acetylcholinesterase using metabolomics and bioactivity assays

Bu-Shen-Huo-Xue Decoction Ameliorates Diabetic Nephropathy by Inhibiting Rac1/PAK1/p38MAPK Signaling Pathway in High-Fat Diet/Streptozotocin-Induced Diabetic Mice

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