Serum paraoxonase is reduced in type 1 diabetic patients compared to non-diabetic, first degree relatives; influence on the ability of HDL to protect LDL from oxidation

Boemi, Massimo; Leviev, Ilia; Sirolla, C.; Pieri, Carlo; Marra, Maurizio; James, Richard

doi:10.1016/s0021-9150(00)00556-6

Cited by 123 publications

(110 citation statements)

References 43 publications

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“…Some studies showed that serum PON1 activity is reduced in diabetes, familial hypercholesterolemia, and kidney diseases that are associated with accelerated atherogenesis [41][42][43] . We also found a positive correlation between PONase/HTase activities and HDL levels, which is consistent with the literature 44) .…”

Section: Discussionmentioning

confidence: 99%

Serum PON-1 Activity but not Q192R Polymorphism is Related to the Extent of Atherosclerosis

Bayrak

Tokgözoğlu

et al. 2012

JAT

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Aim: Paraoxonase-1 (PON1) is an antioxidant enzyme located in high density lipoprotein (HDL). PON1 was defined as a protective factor against atherosclerosis. The aim of this study was to investigate the possible relationship between serum paraoxonase (PONase), homocysteine thiolactonase (HTase) activities and PON1 Q192R polymorphism, and the extent and severity of atherosclerosis. Methods: Blood specimens were collected from 142 individuals who had no coronary artery lesions angiographically (control group) and 128 individuals who had angiographically documented coronary artery disease of several degrees (patient group). The extent and severity of arterial lesions were evaluated by the Gensini scoring system. PONase and HTase activities were measured in serum using a spectrophotometric method. PON1 Q192R polymorphism was evaluated using PCR-RFLP after DNA isolation from blood. Results: Serum PONase and HTase activities were significantly lower in the patient group than in healthy controls (135.7 56.0 U/mL vs 153.8 62.0 U/mL, p 0.05; 36.0 6.1 U/mL vs 43.0 4.04 U/mL, p 0.01; respectively). In the patient group, there was a negative correlation between PONase, HTase activities and the Gensini score (r 0.168, p 0.039; r 0.164, p 0.006, respectively). In both groups, there was no significant difference in the distribution of PON1 Q192R polymorphism. In the patient group, the distribution of Gensini scores according to genotypes was not significant. Conclusion: It has been concluded that serum PONase and HTase activities might be a more relevant marker than PON1 genotype in evaluating the extent and severity of atherosclerosis.

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Section: Discussionmentioning

confidence: 99%

Serum PON-1 Activity but not Q192R Polymorphism is Related to the Extent of Atherosclerosis

Bayrak

Tokgözoğlu

et al. 2012

JAT

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“…The major determinant of serum PON1 concentration was the promoter polymorphism (p < 0.001; 19.9 % of variation in concentration, corresponding to our previous studies [33]). Even quantitatively small changes in serum PON1 modulate the anti-oxidant capacity of HDL [27]. Moreover, such modifications should be considered in the physiological context of atherosclerosis as a slowly evolving, chronic disease and the greater degree of oxidative stress to which diabetic patients are subject.…”

Section: Discussionmentioning

confidence: 99%

The paraoxonase PON1 promoter polymorphism C(-107)T is associated with increased serum glucose concentrations in non-diabetic patients

et al. 2001

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“…22 For statistical analyses, SPSS 11.0 programme was used to evaluate the results. Genotype and allele distribution and differences between groups were analysed by x 2 and Fisher's test.…”

Section: Methodsmentioning

confidence: 99%

“…20 There are several studies of PON1 activity in diabetic patients; most of them have results confirming the decrements in its activity. 21,22 Boemi et al showed that the decrease in the activity would change its antioxidant capacity and concluded that this would be one of the reasons for high oxidant stress in diabetic patients. 22,23 In some studies, it was found that PON1 192A was related to the coronary artery disease in diabetics and PON 55 polymorphisms were related to insulin resistance and destructions on b-cell functions.…”

Section: Introductionmentioning

confidence: 99%

“…21,22 Boemi et al showed that the decrease in the activity would change its antioxidant capacity and concluded that this would be one of the reasons for high oxidant stress in diabetic patients. 22,23 In some studies, it was found that PON1 192A was related to the coronary artery disease in diabetics and PON 55 polymorphisms were related to insulin resistance and destructions on b-cell functions. [24][25][26][27] The reason for PON1 activity decrease in diabetic patients is not fully understood yet; but it is thought that high plasma glucose levels both inactivate PON1 and increase lipid peroxidations on HDL.…”

Section: Introductionmentioning

confidence: 99%

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The relationship of oral disturbances of diabetes mellitus patients with paraoxonase gene polymorphisms

Ünür

Demirez

Ağaçhan

et al. 2008

Cell Biochemistry & Function

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Diabetes Mellitus (DM) is a multisystemic disorder with serious complications and these patients may also have serious problems with their oral cavity probably because of the microangiopathic and neuropathic complications. In diabetic patients, there may be several problems of the oral cavity such as gingivitis, periodontitis, candidiasis, glossitis, oral ulcerations, loss of taste sensations, opportunistic infections and several other conditions dependent on these. One of the recent theories about complications in DM is the contribution of reactive oxygen radicals. Paraoxonase (PON1) is an enzyme that is synthesized in liver and having the capability of hydrolasing the active metabolite of an insectisid, parathion. Previously it was shown that there are two polymorphic areas on the PON1 gene: one causing a Leu ! Met substitution at 55th position, the other causing Gln ! Arg at the 192nd position. We investigated the differences in PON activities related to the oral lesions in Type 2 diabetics and control subjects to see their relationships with PON1 activity levels and the two main gene polymorphisms of PON1 genes, PON1 192 and PON1 55. We had 51 patients and 53 healthy subjects used in the study. PON activity was significantly decreased in Type 2 DM group compared to the control group. Neither PON1 192 nor PON1 55 genotypes had any differential effect on PON1 enzyme activity levels in either group. However, we found that PON1 55 M allele carriers had greater risk for general periodontal and/or gingival problems.

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Serum paraoxonase is reduced in type 1 diabetic patients compared to non-diabetic, first degree relatives; influence on the ability of HDL to protect LDL from oxidation

Cited by 123 publications

References 43 publications

Serum PON-1 Activity but not Q192R Polymorphism is Related to the Extent of Atherosclerosis

Serum PON-1 Activity but not Q192R Polymorphism is Related to the Extent of Atherosclerosis

The paraoxonase PON1 promoter polymorphism C(-107)T is associated with increased serum glucose concentrations in non-diabetic patients

The relationship of oral disturbances of diabetes mellitus patients with paraoxonase gene polymorphisms

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