Serum Paraoxonase/Arylesterase Activity Affects Outcome in Ischemic Stroke Patients

Abstract

Background: The severity of neurological deficits arising from ischemic stroke may be related to serum redox homeostasis. The aim of this study was to estimate the effect of serum paraoxonase (PON), arylesterase (ARE) activities and conjugated dienes (CD) on patient outcome during a 1-year follow-up period. Methods: The study included 468 consecutive ischemic stroke patients (251 males, 217 females) with an average age of 67.5 ± 12.4 years. Clinical evaluation was based on vital signs, National Institutes of Health Stroke Scale (NIHSS) scored at the time of admission and on the 7th day after stroke, as well as modified Rankin scale (mRS) and Barthel index (BI) scored at 30, 90, 180 and 360 days after stroke onset. Serum PON, ARE activities and CD concentration were measured with the use of spectrophotometric methods. Results: Serum PON activity alone correlated directly with a favorable outcome during a 3-month observation period. Serum ARE activity correlated directly only with the mRS score in a 1-year observation. PON/ARE ratio showed the strongest direct correlation with favorable stroke outcome expressed by BI and inverse correlation with mRS as compared to serum PON or ARE activities assessed alone. PON/ARE affected the NIHSS score on admission (rS = –0.119, p = 0.014) and on the 7th day after stroke (rS = 0.120, p = 0.015); it also showed an association with the BI and mRS on the 30th (rS = 0.145, p = 0.007 and rS = –0.098, p = 0.049, respectively), 90th (rS = 0.147, p = 0.009, rS = –0.133, p = 0.008, respectively), as well as 180th, and 360th day after stroke. We did not find correlations between the serum CD concentration and stroke outcome. Conclusion: The PON/ARE ratio is an important predictor of ischemic stroke outcome and can be used in clinical practice rather than evaluating either PON or ARE activity alone.

Paraoxonase 1 in neurological disorders

“…67 In 2011, Michalak et al studied the association of PON1 triesterase ( paraoxon) and arylesterase activities and conjugated dienes on stroke patient outcome during a 1-year follow-up period. 68 They concluded that the PON/aryl ratio is a significant predictor of ischemic stroke outcome and could be used in the clinical setting to better advantage than the paraoxon or aryl assays alone. These findings have been confirmed by recently Sand.…”

The Early Effect of Carotid Artery Stenting on Antioxidant Capacity and Oxidative Stress in Patients with Carotid Artery Stenosis

“…Ferretti et al observed that disturbed PON activity was associated with severe neurological deficit [31]. In our previous study we have found the effects of impaired PON and ARE activities on ischemic stroke outcome during short- and long-term observation [16]. Moreover, we have identified the predictive value of PON to ARE ratio [16].…”

“…In our previous study we have found the effects of impaired PON and ARE activities on ischemic stroke outcome during short- and long-term observation [16]. Moreover, we have identified the predictive value of PON to ARE ratio [16]. We are not aware of the studies which investigated the effects of TIA on PON activities.…”

“…PON and ARE belong to enzymatic antioxidant system in plasma and hydrolytic reactions catalyzed by the enzymes, which are crucial for protection against peroxidation of plasma lipids. In our previous study we have identified PON and ARE activities and PON/ARE ratio as predictors of the outcome after ischemic stroke [16]. …”

Comparison of serum paraoxonase and arylesterase activities between iron deficiency anemia patients and chronic kidney disease patients with anemia

“…31 Michalak et al emphasized the importance and usefulness of PON/ARE ratio in predicting risk of ischemic stroke in their study. 32 Therefore, we also tried to compare PON/ARE ratio between groups but we could not have determined a correlation between CIMT and PON/ ARE ratio. Also in our study, CIMT was significantly different only between CKD patients and healthy subjects.…”