Serum parameters in the spectrum of coeliac disease: beyond standard antibody testing - a cohort study

Tack, Greetje J.; Wanrooij, Roy L.J. van; Blomberg, B. Mary E. von; Amini, Hedayat; Coupé, Veerle M.H.; Bonnet, Petra; Mulder, Chris J.; Schreurs, Marco W. J.

doi:10.1186/1471-230x-12-159

Cited by 20 publications

(15 citation statements)

References 43 publications

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“…The immune‐mediated inflammation associated with untreated CD normalizes after long‐term GFD in most patients. The stage of the disease can be reflected by specific serology and several inflammatory markers . Although untreated patients are not included in our study, significant differences were observed among treated patients with CD, according to the time of GFD adherence.…”

Section: Resultsmentioning

confidence: 90%

“…TG2 expression and activity is increased in the small intestine of patients with active disease; in addition, extradigestive TG2 dysfunction can contribute to extradigestive disorders . The inflammatory status of CD can be systemically reflected by the increase in various serological parameters; after long‐term adhesion to gluten‐free diet (GFD), these inflammatory parameters and specific serology declines in line with gradual gut mucosal recovery indicating that gluten triggers the disease …”

Section: Introductionmentioning

confidence: 99%

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Alternative RNA splicing of leucocyte tissue transglutaminase in coeliac disease

et al. 2018

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Tissue transglutaminase is a ubiquitous and multifunctional protein that contributes to several processes such as apoptosis/survival, efferocytosis, inflammation and tissue repairing under physiological and pathological conditions. Several activities can be associated with well-established functional domains; in addition, four RNA alternative splice variants have been described, characterized by sequence divergences and residues deletion at the C-terminal domains. Tissue transglutaminase is recognized as the central player in the physiopathology of coeliac disease (CD) mainly through calcium-dependent enzymatic activities. It can be hypothesized that differential regulation of tissue transglutaminase splice variants expression in persons with CD contributes to pathology by altering the protein functionality. We characterized the expression pattern of RNA alternative splice variants by RT-PCR in peripheral cells from patients with CD under free gluten diet adhesion; we considered inflammatory parameters and specific antibodies as markers of the stage of disease. We found significant higher expression of both the full length and the shortest C-truncated splice variants in leucocytes from patients with CD in comparison with healthy individuals. As tissue transglutaminase expression and canonical enzymatic activity are linked to inflammation, we studied the RNA expression of inflammatory cytokines in peripheral leucocytes of persons with CD in relation with splice variants expression; interestingly, we found that recently diagnosed patients showed significant correlation between both the full length and the shortest alternative spliced variants with IL-1 expression. Our results points that regulation of alternative splicing of tissue transglutaminase could account for the complex physiopathology of CD.

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Section: Resultsmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Alternative RNA splicing of leucocyte tissue transglutaminase in coeliac disease

et al. 2018

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“…In an attempt to shed light on this issue, we studied cell subsets in the epithelium of RCDI patients and cytokine profile in the peripheral circulation and found these to be similar to uncomplicated active CD but dissimilar from RCDII patients [34, 35]. …”

Section: Pathogenesismentioning

confidence: 99%

Update on the Diagnosis and Management of Refractory Coeliac Disease

Nijeboer

Wanrooij

Tack

et al. 2013

Gastroenterology Research and Practice

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A small subset of coeliac disease (CD) patients experiences persisting or recurring symptoms despite strict adherence to a gluten-free diet (GFD). When other causes of villous atrophy have been excluded, these patients are referred to as refractory celiac disease (RCD) patients. RCD can be divided in two types based on the absence (type I) or presence (type II) of an, usually clonal, intraepithelial lymphocyte population with aberrant phenotype. RCDI usually runs a benign course and may be difficult to be differentiated from uncomplicated, slow responding CD. In contrast, RCDII can be defined as low-grade intraepithelial lymphoma and frequently transforms into an aggressive enteropathy associated T-cell lymphoma with dismal prognosis. This paper describes the clinical characteristics of RCDI and RCDII, diagnostic approach, and the latest insights in treatment options.

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“…This is in line with our results where children with CD and T1D had elevated IFN- and IL-17 levels. Furthermore, elevated serum levels of IL-8 have been reported in patients with active CD compared to GFD-treated CD [392]. SOCS3 has previously been reported to be elevated upon in vitro stimulation of pancreatic islets with proinflammatory cytokines (such as IFN-, IL-1and TNF-) and also in the inflamed pancreas of T1D patients [393].…”

Section: Mucosal Immune Responses In T1dmentioning

confidence: 99%

Studies of Mucosal Immune Regulation in Celiac Disease and Type 1 Diabetes

Lahdenperä¹

2014

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Serum parameters in the spectrum of coeliac disease: beyond standard antibody testing - a cohort study

Cited by 20 publications

References 43 publications

Alternative RNA splicing of leucocyte tissue transglutaminase in coeliac disease

Alternative RNA splicing of leucocyte tissue transglutaminase in coeliac disease

Update on the Diagnosis and Management of Refractory Coeliac Disease

Studies of Mucosal Immune Regulation in Celiac Disease and Type 1 Diabetes

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