Serum oxytocinase activity is related to tumor growth parameters in N-methyl nitrosourea induced rat breast cancer

Carrera, Marı́a Pilar; Ramírez‐Expósito, María Jesús; Valenzuela, Marı́a Teresa; García, María Jesús García; Mayas, Marı́a Dolores; Martı́nez-Martos, José Manuel

doi:10.1016/j.lfs.2004.04.009

Cited by 12 publications

(6 citation statements)

References 19 publications

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“…The hormone oxytocin is common in cells of healthy breast tissue, but is rarely or never detected in BC in vitro and in vivo . Oxytocin inhibits proliferation of human BC cell lines, and thus may play a role in preventing this disease37.…”

Section: Discussionmentioning

confidence: 99%

Gene expression profiling of breast cancer in Lebanese women

Makoukji

Makhoul

Khalil

et al. 2016

Sci Rep

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Breast cancer is commonest cancer in women worldwide. Elucidation of underlying biology and molecular pathways is necessary for improving therapeutic options and clinical outcomes. Molecular alterations in breast cancer are complex and involve cross-talk between multiple signaling pathways. The aim of this study is to extract a unique mRNA fingerprint of breast cancer in Lebanese women using microarray technologies. Gene-expression profiles of 94 fresh breast tissue samples (84 cancerous/10 non-tumor adjacent samples) were analyzed using GeneChip Human Genome U133 Plus 2.0 arrays. Quantitative real-time PCR was employed to validate candidate genes. Differentially expressed genes between breast cancer and non-tumor tissues were screened. Significant differences in gene expression were established for COL11A1/COL10A1/MMP1/COL6A6/DLK1/S100P/CXCL11/SOX11/LEP/ADIPOQ/OXTR/FOSL1/ACSBG1 and C21orf37. Pathways/diseases representing these genes were retrieved and linked using PANTHER®/Pathway Studio®. Many of the deregulated genes are associated with extracellular matrix, inflammation, angiogenesis, metastasis, differentiation, cell proliferation and tumorigenesis. Characteristics of breast cancers in Lebanese were compared to those of women from Western populations to explain why breast cancer is more aggressive and presents a decade earlier in Lebanese victims. Delineating molecular mechanisms of breast cancer in Lebanese women led to key genes which could serve as potential biomarkers and/or novel drug targets for breast cancer.

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Section: Discussionmentioning

confidence: 99%

Gene expression profiling of breast cancer in Lebanese women

Makoukji

Makhoul

Khalil

et al. 2016

Sci Rep

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“…It is possible to attribute this effect to oleic acid, although the mechanism involved is unknown. In previous work, we demonstrated the existence of high levels of specific oxytocinase activity in the serum of rats with NMU-induced breast cancer (Carrera et al, 2004b). This activity correlated with the number and size of tumors and body weight.…”

Section: Dietary Fat Weight Gain and Tumor Growth Parametersmentioning

confidence: 71%

Dietary Fat Including Olive Oil and Breast Cancer in the N-methyl Nitrosourea (NMU) Animal Model

Ramírez-Expósito

Carrera

Cortés

et al. 2010

Olives and Olive Oil in Health and Disease Prevention

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“…Body weight decreased after this time when compared with control rats. These data regarding tumour growth parameters and the tumour number per rat as well as major and minor diameters of tumour have been previously published [21].…”

Section: Animal and Treatmentsmentioning

confidence: 99%

“…Soluble and membrane-bound IRAP activities were measured fluorometrically using cystyl-β-naphthylamide (CysNNap) as the substrate, according to the method previously described by us [21]. Briefly, 10 μl of each sample was incubated in triplicate during 30 min at 37°C with 100 μl of the substrate solution containing 100 μM CysNNap and 0.65 μM dithiothreitol (DTT) in 50 mM of phosphate buffer, pH 6.0.…”

Section: Insulin-regulated Aminopeptidase Activity Assaymentioning

confidence: 99%

“…Changes in IRAP activity have been reported in both human breast cancer [20] and N-methyl-nitrosourea (NMU)-induced rat model of breast cancer [21]. In this model, serum IRAP activity correlates with the number and size of the tumours and with the body weight of the animals, also suggesting the possibility of the existence of hypothyroidism.…”

Section: Introductionmentioning

confidence: 97%

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Hypothalamus–pituitary–thyroid axis disruption in rats with breast cancer is related to an altered endogenous oxytocin/insulin-regulated aminopeptidase (IRAP) system

et al. 2011

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Associations of breast cancer with diseases of the thyroid have been repeatedly reported, but the mechanism underlying this association remains to be elucidated. It has been reported that oxytocin (OXT) attenuates the thyroid-stimulating hormone (TSH) release in response to thyrotrophin-releasing hormone (TRH) and decreased plasma levels of TSH as well as the thyroid hormones by an effect mediated by the central nervous system. Oxytocinase (IRAP) is the regulatory proteolytic enzyme reported to hydrolyze OXT. Changes in IRAP activity have been reported in both human breast cancer and N-methyl-nitrosourea (NMU)-induced rat mammary tumours. Here, we measure IRAP activity fluorometrically using cystyl-β-naphthylamide as the substrate, in the hypothalamus-pituitary-thyroid axis together with the circulating levels of OXT, and its relationship with circulating levels of TSH and free thyroxine (fT4), as markers of thyroid function in control rats and rats with breast cancer induced by NMU. We found decreased thyroid function in rats with breast cancer induced by NMU, supported by the existence of lower serum circulating levels of both TSH and fT4 than their corresponding controls. Concomitantly, we found a decrease of hypothalamic IRAP activity and an increase in circulating levels of OXT. We propose that breast cancer increases OXT pituitary release by decreasing its hypothalamic catabolism through IRAP activity, probably due to the alteration of the estrogenic endocrine status. Thus, high circulating levels of OXT decreased TSH release from the pituitary, and therefore, of thyroid hormones from the thyroid, supporting the association between breast cancer and thyroid function disruption.

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Serum oxytocinase activity is related to tumor growth parameters in N-methyl nitrosourea induced rat breast cancer

Cited by 12 publications

References 19 publications

Gene expression profiling of breast cancer in Lebanese women

Gene expression profiling of breast cancer in Lebanese women

Dietary Fat Including Olive Oil and Breast Cancer in the N-methyl Nitrosourea (NMU) Animal Model

Hypothalamus–pituitary–thyroid axis disruption in rats with breast cancer is related to an altered endogenous oxytocin/insulin-regulated aminopeptidase (IRAP) system

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