Serum OPN Expression for Identification of Gastric Cancer and Atrophic Gastritis and Its Influencing Factors

Abstract

BackgroundMost studies have found that osteopontin (OPN) expression level is related to the poor prognosis of gastric cancer. However, few studies have examined the relationship between OPN expression and gastric precancerous diseases, and the potential role of OPN in the formation and development of GC. We investigated the relationships between serum OPN levels and the risks of gastric cancer (GC) and its precancerous disease, to explore the diagnostic efficacy of serum OPN level for GC and atrophic gastritis and its influencing factors.MethodsA total of 1,452 patients were enrolled, including 609 with mild superficial gastritis (SG), 594 with atrophic gastritis (AG) and 249 with GC. The levels of serum OPN and serum Helicobacter pylori IgG antibody were detected by enzyme-linked immunosorbent assay.ResultsSerum OPN levels increased from mild SG (1.99±1.91 ng/ml) to AG (2.37±2.27 ng/ml) to GC (5.94±4.52 ng/ml) (P≤0.002), along with increasing severity of gastric disease. OPN levels were significantly higher in patients with GC compared with the non-cancer population (2.17±2.10, P<0.0001). Serum OPN level was positively correlated with age and was higher in men than women, but was not correlated with H. pylori infection status. The area under the receiver operating characteristic curve was 0.805, the optimal cutoff was 2.56 ng/ml and the sensitivity and specificity were 74.3% and 71.8%, respectively, for the ability of serum OPN to discriminate GC.ConclusionsSerum OPN expression was closely related to the risks of GC and AG, and it might be a useful marker for the discrimination of GC. OPN level was positively correlated with age and male sex, but was not affected by H. pylori infection, and it was promoted by smoking and drinking, in patients with mild SG.

Three-dimensional combined biomarkers assay could improve diagnostic accuracy for gastric cancer

“…For the one-dimensional models, according to the results of our previous research, the cutoff values were set 7.0 for PGI/II 6 , 34.0 EIU for HpAb [positive, Biohit], and 2.6 ng/mL for OPN 16 , respectively. For the multi-dimensional models, the optimal cutoff points were calculated based on the highest specificity with sensitivity >0.7.…”

“…Over the past decade, compelling evidence from experimental and clinical studies suggested that OPN overexpression was associated with clinic-pathological features and prognosis of GC 29 – 31 . Our previous research, which focused on investigating the relationships between serum OPN levels and risks of GC together with its influencing factors, has found serum OPN expression was closely related to GC risks, suggested that it might be a useful marker for the discrimination of GC 16 . However, as OPN is widely expressed in a variety of tissues, including bone, cartilage, kidney, blood vessels, skin and other tissue 32 – 35 , it itself lacks organ specificity and can’t be positioned.…”

“…The latter includes those closely related to the occurrence and progression of gastrointestinal cancers (e.g., carcinoembryonic antigen, CA 19-9, CA 72-4, E-cadherin, Mg7 and osteopontin (OPN)) which may be indicative of the presence of malignancy, but not specific of the organ from which the malignancy arises 13 – 15 . In our previous study, we found serum OPN levels, a multifunctional glycophosphoprotein, increased gradually when the gastric mucosa progressed from superficial gastritis to atrophic gastritis, and to GC 16 .…”

“…Participants with a histopathological diagnose of mild superficial gastritis were recruited as controls (n = 729). Furthermore, considering the influence of age and gender on serum levels of PG and OPN 6 , 16 , a subset of 332 GC cases was selected 1:1 matched control on sex and age (within 5 years) for subsequent comparative analysis.…”

An Observational Study on Aberrant Methylation of Runx3 With the Prognosis in Chronic Atrophic Gastritis Patients

“… 1 There is no specific clinical manifestations among CAG patients; however, most patients have the clinical symptoms of pain, fullness, nausea, belching, constipation, and diarrhea in the epigastrium. 1 , 2 Recently, the incidence, prevalence, and mortality of CAG in China have been found to be growing because of the increasing infection rate of helicobacter pylori (Hp), aggravation of environmental pollution, and unhealthy lifestyles such as high-fat and high-salt diet, shortage of fruits, and vegetables intake. 3 Although the pathogenesis of CAG is still unclear, many research works have proved that long-term changes in gastric mucosa of CAG are one of the risk factors for gastric cancer (GC).…”