Serum neopterin, sialic acid and nitric oxide levels in dogs with malignant mammary tumours

Abstract

Mammary cancer is one of the leading causes of death in pet population. Early diagnosis and malignancy detection is important for prognosis. The levels of neopterin, sialic acid and nitric oxide in serum of dogs with malignant mammary tumours were evaluated to investigate the importance of these biochemical parameters for malign mammary tumour. Twelve healthy dogs and twenty dogs with malignant mammary tumours were used as research materials. Blood samples were collected from both groups for neopterin analysis by enzyme-linked immunosorbent assay, whereas nitric oxide and sialic acid were measured by modified nitrate reductase method and spectrophotometry, respectively. Tissue specimens were evaluated and defined as malignant tumours. Serum nitric oxide and sialic acid levels in dogs with mammary tumours were significantly higher than those in the healthy dogs. Serum neopterin levels were not found significantly different in dogs with mammary tumours compared to healthy dogs. Malignancy of canine mammary tumours are accompained by an elevation of nitric oxide and sialic acid levels.

Heat shock protein 70 and nitric oxide concentrations in non-tumorous and neoplastic canine mammary tissues: preliminary results – Short communication

“…Our findings are consistent with the results of the studies performed by Askar et al (2009) and Tascene et al (2011), who demonstrated a significantly higher level of NO in the serum of female dogs with malignant mammary gland tumours as compared to healthy dogs.…”

Serum neopterin levels in female dogs with malignant mammary tumours

“…According to the studies performed recently by Tascene et al . there were no significant differences between serum neopterin levels in female dogs with malignant mammary tumours and healthy animals . This study involved only 20 female dogs with malignant mammary tumours (classified according to Moulton (1990) histopathological classification of canine mammary tumours), and were mainly tubular malignant mixed tumours.…”

“…The data regarding neopterin concentration in female dogs with mammary tumours are scarce. According to the studies performed recently by Tascene et al . there were no significant differences between serum neopterin levels in female dogs with malignant mammary tumours and healthy animals .…”

“…Moreover, elevated levels of neopterin were demonstrated in cases of bacterial infections and babesiosi . As yet, the data on neopterin levels in dogs with neoplasms have been scarce …”

Urinary neopterin, a non-invasive marker of mammalian cellular immune activation, is highly stable under field conditions

“…Although some authors have suggested that urinary NEO is only produced in detectable forms and concentrations in humans and nonhuman primates 31 44 , a number of studies of stress and immune responses in other mammals have included measurements of serum NEO, suggesting the potential for much broader applicability (e.g. dogs 45 ; pigs 46 ; cattle, horses, lamas, dogs, cats and rats 47 ).…”