Serum miRNAs panel (miR-16-2*, miR-195, miR-2861, miR-497) as novel non-invasive biomarkers for detection of cervical cancer

Zhang, Yujuan; Zhang, Donghong; Wang, Fei; Xu, Danfei; Guo, Ye; Cui, Wei

doi:10.1038/srep17942

Cited by 69 publications

(69 citation statements)

References 41 publications

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“…For example, Zhang et al have reported miR-195 as a novel biomarker for detection of CC [5]. In line with these reports, our research displayed the same results based on bioinformatics analysis and in vitro experiments.…”

Section: Cellular Physiology and Biochemistry Cellular Physiology Andsupporting

confidence: 81%

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The Effects of miR-195-5p/MMP14 on Proliferation and Invasion of Cervical Carcinoma Cells Through TNF Signaling Pathway Based on Bioinformatics Analysis of Microarray Profiling

Ren

Zhang

et al. 2018

Cell Physiol Biochem

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Background/Aims: This study is aimed at identification of miR-195-5p/MMP14 expression in cervical cancer (CC) and their roles on cell proliferation and invasion profile of CC cells through TNF signaling pathway in CC. Methods: Microarray analysis, gene set enrichment analysis (GSEA) and DAVID were used to analyze differentially expressed miRNAs, mRNAs and signaling pathways. MiR-195-5p and MMP14 expression levels in CC cell were determined by qRT-PCR. Western blot was employed to measure MMP14 and TNF signaling pathway-relating protein level. Luciferase reporter system was used to confirm the targeting relationship between MMP14 and miR-195-5p. Cell proliferation and invasion was respectively deeded by CCK8, transwell. In vivo experiment was carried out to study the impact of MMP14 and miR-195-5p on CC development in mice. Results: The microarray analysis and the results of qRT-PCR determined that miR-195-5p was under-expressed and MMP14 was over-expressed in CC cells. GSEA and DAVID analysis showed that TNF signaling pathway was regulated by miR-195-5p/MMP14 and activated in cervical carcinoma cells. The miR-195-5p and MMP14 have a negative regulation relation. In vivo experiment found that down-regulated MMP14 and up-regulated miR-195-5p suppressed the tumor development. Conclusion: Our results suggest that MMP14 is a direct target of miR-195-5p, and down-regulated MMP14 and up-regulated miR-195-5p suppressed proliferation and invasion of CC cells by inhibiting TNF signaling pathway.

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Section: Cellular Physiology and Biochemistry Cellular Physiology Andsupporting

confidence: 81%

“…They are reported to regulate protein expressions by binding to specific genes and inhibiting mRNA or protein translation process [2,5]. Numerous researches have demonstrated that miRNA expression level is closely related to human cancer progression and metastasis.…”

Section: Introductionmentioning

confidence: 99%

The Effects of miR-195-5p/MMP14 on Proliferation and Invasion of Cervical Carcinoma Cells Through TNF Signaling Pathway Based on Bioinformatics Analysis of Microarray Profiling

Ren

Zhang

et al. 2018

Cell Physiol Biochem

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“…(2015) identified 66 circulating miRNAs that were upregulated, while 32 circulating miRNAs were downregulated in serum samples of ovarian cancer patients compared with serum samples from healthy controls. More specifically, miR‐497, miR‐16‐2*, miR‐195, and miR‐2861 were expressed at significantly higher levels in serum samples of ovarian cancer patients compared to cervical intraepithelial neoplasia patients and healthy control subjects (Zhang et al ., 2015). Xu et al .…”

Section: Circulating Mirnas As Biomarkersmentioning

confidence: 99%

Cell‐to‐cell communication: microRNAs as hormones

2017

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Mammalian cells can release different types of extracellular vesicles (EVs), including exosomes, microvesicles, and apoptotic bodies. Accumulating evidence suggests that EVs play a role in cell‐to‐cell communication within the tumor microenvironment. EVs’ components, such as proteins, noncoding RNAs [microRNAs (miRNAs), and long noncoding RNAs (lncRNAs)], messenger RNAs (mRNAs), DNA, and lipids, can mediate paracrine signaling in the tumor microenvironment. Recently, miRNAs encapsulated in secreted EVs have been identified in the extracellular space. Mature miRNAs that participate in intercellular communication are released from most cells, often within EVs, and disseminate through the extracellular fluid to reach remote target cells, including tumor cells, whose phenotypes they can influence by regulating mRNA and protein expression either as tumor suppressors or as oncogenes, depending on their targets. In this review, we discuss the roles of miRNAs in intercellular communication, the biological function of extracellular miRNAs, and their potential applications for diagnosis and therapeutics. We will give examples of miRNAs that behave as hormones.

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“…11 MiRNAs display several properties that make them potentially valuable biomarkers including stability in body fluids, resistance to endogenous RNase activity, prolonged life at room temperature, and resistance to multiple freeze-thaw cycles. 12 A growing body of evidence shows a different pattern of expression of circulating miRNAs between cancer patients and Identification of plasma microRNAs as new potential biomarkers with high diagnostic power in human cutaneous melanoma healthy donors 11,[13][14][15][16][17][18][19] ; however, only a limited number of studies have been focused on melanoma.…”

Section: Introductionmentioning

confidence: 99%

Identification of plasma microRNAs as new potential biomarkers with high diagnostic power in human cutaneous melanoma

et al. 2017

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Melanoma is a devastating disease with few therapeutic options in the advanced stage and with the urgent need of reliable biomarkers for early detection. In this context, circulating microRNAs are raising great interest as diagnostic biomarkers. We analyzed the expression profiles of 21 selected microRNAs in plasma samples from melanoma patients and healthy donors to identify potential diagnostic biomarkers. Data analysis was performed using global mean normalization and NormFinder algorithm. Linear regression followed by receiver operating characteristic analyses was carried out to evaluate whether selected plasma miRNAs were able to discriminate between cases and controls. We found five microRNAs that were differently expressed among cases and controls after Bonferroni correction for multiple testing. Specifically, miR-15b-5p, miR-149-3p, and miR-150-5p were up-regulated in plasma of melanoma patients compared with healthy controls, while miR-193a-3p and miR-524-5p were down-regulated. Receiver operating characteristic analyses of these selected microRNAs provided area under the receiver operating characteristic curve values ranging from 0.80 to 0.95. Diagnostic value of microRNAs is improved when considering the combination of miR-149-3p, miR-150-5p, and miR-193a-3p. The triple classifier had a high capacity to discriminate between melanoma patients and healthy controls, making it suitable to be used in early melanoma diagnosis.

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Serum miRNAs panel (miR-16-2*, miR-195, miR-2861, miR-497) as novel non-invasive biomarkers for detection of cervical cancer

Cited by 69 publications

References 41 publications

The Effects of miR-195-5p/MMP14 on Proliferation and Invasion of Cervical Carcinoma Cells Through TNF Signaling Pathway Based on Bioinformatics Analysis of Microarray Profiling

The Effects of miR-195-5p/MMP14 on Proliferation and Invasion of Cervical Carcinoma Cells Through TNF Signaling Pathway Based on Bioinformatics Analysis of Microarray Profiling

Cell‐to‐cell communication: microRNAs as hormones

Identification of plasma microRNAs as new potential biomarkers with high diagnostic power in human cutaneous melanoma

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